Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0620 and Shewana3_0621 are separated by 205 nucleotides Shewana3_0621 and Shewana3_0622 are separated by 171 nucleotides Shewana3_0622 and Shewana3_0623 are separated by 77 nucleotides
Shewana3_0620: Shewana3_0620 - diguanylate cyclase/phosphodiesterase (RefSeq), at 724,932 to 727,118
_0620
Shewana3_0621: Shewana3_0621 - hypothetical protein (RefSeq), at 727,324 to 727,881
_0621
Shewana3_0622: Shewana3_0622 - ATP-dependent helicase HrpB (RefSeq), at 728,053 to 730,560
_0622
Shewana3_0623: Shewana3_0623 - penicillin-binding protein 1B (RefSeq), at 730,638 to 732,932
_0623
Position (kb) 728
729
730
731 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 727.071 kb on - strand at 727.087 kb on - strand at 727.088 kb on - strand at 727.088 kb on - strand at 727.095 kb on - strand at 727.410 kb on - strand, within Shewana3_0621 at 727.504 kb on - strand, within Shewana3_0621 at 727.627 kb on + strand, within Shewana3_0621 at 727.637 kb on - strand, within Shewana3_0621 at 727.659 kb on - strand, within Shewana3_0621 at 727.814 kb on - strand, within Shewana3_0621 at 727.864 kb on - strand at 727.999 kb on + strand at 727.999 kb on + strand at 728.007 kb on - strand at 728.166 kb on - strand at 728.171 kb on - strand at 728.300 kb on - strand at 728.327 kb on - strand, within Shewana3_0622 at 728.434 kb on - strand, within Shewana3_0622 at 728.437 kb on + strand, within Shewana3_0622 at 728.476 kb on + strand, within Shewana3_0622 at 728.577 kb on + strand, within Shewana3_0622 at 728.579 kb on - strand, within Shewana3_0622 at 728.634 kb on + strand, within Shewana3_0622 at 728.754 kb on + strand, within Shewana3_0622 at 728.756 kb on + strand, within Shewana3_0622 at 728.756 kb on + strand, within Shewana3_0622 at 728.824 kb on + strand, within Shewana3_0622 at 728.824 kb on + strand, within Shewana3_0622 at 728.829 kb on + strand, within Shewana3_0622 at 728.837 kb on - strand, within Shewana3_0622 at 728.837 kb on - strand, within Shewana3_0622 at 728.980 kb on + strand, within Shewana3_0622 at 728.981 kb on + strand, within Shewana3_0622 at 728.987 kb on + strand, within Shewana3_0622 at 729.145 kb on + strand, within Shewana3_0622 at 729.145 kb on + strand, within Shewana3_0622 at 729.153 kb on - strand, within Shewana3_0622 at 729.153 kb on - strand, within Shewana3_0622 at 729.192 kb on + strand, within Shewana3_0622 at 729.265 kb on + strand, within Shewana3_0622 at 729.376 kb on + strand, within Shewana3_0622 at 729.376 kb on + strand, within Shewana3_0622 at 729.409 kb on - strand, within Shewana3_0622 at 729.463 kb on + strand, within Shewana3_0622 at 729.481 kb on - strand, within Shewana3_0622 at 729.481 kb on - strand, within Shewana3_0622 at 729.631 kb on + strand, within Shewana3_0622 at 729.679 kb on + strand, within Shewana3_0622 at 730.016 kb on - strand, within Shewana3_0622 at 730.234 kb on - strand, within Shewana3_0622 at 730.447 kb on - strand at 730.561 kb on + strand at 730.686 kb on - strand at 730.786 kb on + strand at 730.865 kb on - strand at 730.874 kb on + strand, within Shewana3_0623 at 730.901 kb on - strand, within Shewana3_0623 at 731.037 kb on + strand, within Shewana3_0623 at 731.231 kb on - strand, within Shewana3_0623 at 731.292 kb on - strand, within Shewana3_0623 at 731.299 kb on + strand, within Shewana3_0623 at 731.376 kb on + strand, within Shewana3_0623 at 731.384 kb on - strand, within Shewana3_0623 at 731.455 kb on - strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.509 kb on - strand, within Shewana3_0623 at 731.526 kb on + strand, within Shewana3_0623
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 727,071 - +0.5 727,087 - -1.1 727,088 - +1.3 727,088 - -1.6 727,095 - +0.5 727,410 - Shewana3_0621 0.15 +0.1 727,504 - Shewana3_0621 0.32 -1.6 727,627 + Shewana3_0621 0.54 +0.3 727,637 - Shewana3_0621 0.56 -2.0 727,659 - Shewana3_0621 0.60 -1.3 727,814 - Shewana3_0621 0.88 +1.0 727,864 - -1.4 727,999 + -1.5 727,999 + +2.9 728,007 - -2.5 728,166 - -0.1 728,171 - +0.1 728,300 - +0.3 728,327 - Shewana3_0622 0.11 +0.7 728,434 - Shewana3_0622 0.15 +0.6 728,437 + Shewana3_0622 0.15 -1.5 728,476 + Shewana3_0622 0.17 +0.3 728,577 + Shewana3_0622 0.21 +0.2 728,579 - Shewana3_0622 0.21 +0.8 728,634 + Shewana3_0622 0.23 +0.5 728,754 + Shewana3_0622 0.28 +0.4 728,756 + Shewana3_0622 0.28 +0.8 728,756 + Shewana3_0622 0.28 -0.7 728,824 + Shewana3_0622 0.31 +0.6 728,824 + Shewana3_0622 0.31 -1.3 728,829 + Shewana3_0622 0.31 -1.1 728,837 - Shewana3_0622 0.31 +0.4 728,837 - Shewana3_0622 0.31 +0.3 728,980 + Shewana3_0622 0.37 -1.3 728,981 + Shewana3_0622 0.37 -1.3 728,987 + Shewana3_0622 0.37 -1.2 729,145 + Shewana3_0622 0.44 +0.2 729,145 + Shewana3_0622 0.44 -1.5 729,153 - Shewana3_0622 0.44 -1.1 729,153 - Shewana3_0622 0.44 +0.1 729,192 + Shewana3_0622 0.45 +1.3 729,265 + Shewana3_0622 0.48 -0.9 729,376 + Shewana3_0622 0.53 +0.3 729,376 + Shewana3_0622 0.53 +0.2 729,409 - Shewana3_0622 0.54 +0.6 729,463 + Shewana3_0622 0.56 -0.9 729,481 - Shewana3_0622 0.57 -0.8 729,481 - Shewana3_0622 0.57 -0.1 729,631 + Shewana3_0622 0.63 -1.2 729,679 + Shewana3_0622 0.65 -0.2 730,016 - Shewana3_0622 0.78 +0.5 730,234 - Shewana3_0622 0.87 +0.1 730,447 - -1.5 730,561 + +0.2 730,686 - -2.4 730,786 + -1.9 730,865 - -3.3 730,874 + Shewana3_0623 0.10 +3.2 730,901 - Shewana3_0623 0.11 -1.8 731,037 + Shewana3_0623 0.17 -2.4 731,231 - Shewana3_0623 0.26 -1.5 731,292 - Shewana3_0623 0.28 -1.3 731,299 + Shewana3_0623 0.29 -1.6 731,376 + Shewana3_0623 0.32 -1.3 731,384 - Shewana3_0623 0.33 -1.3 731,455 - Shewana3_0623 0.36 -0.1 731,482 + Shewana3_0623 0.37 -0.6 731,482 + Shewana3_0623 0.37 -0.4 731,482 + Shewana3_0623 0.37 -1.9 731,509 - Shewana3_0623 0.38 -1.7 731,526 + Shewana3_0623 0.39 -1.6
Or see this region's nucleotide sequence