Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0544 and Shewana3_0545 are separated by 166 nucleotides Shewana3_0545 and Shewana3_0546 are separated by 62 nucleotides
Shewana3_0544: Shewana3_0544 - response regulator receiver modulated diguanylate cyclase/phosphodiesterase (RefSeq), at 638,965 to 640,683
_0544
Shewana3_0545: Shewana3_0545 - alpha-L-glutamate ligase (RefSeq), at 640,850 to 641,755
_0545
Shewana3_0546: Shewana3_0546 - hypothetical protein (RefSeq), at 641,818 to 642,510
_0546
Position (kb) 640
641
642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 640.114 kb on + strand, within Shewana3_0544 at 640.158 kb on - strand, within Shewana3_0544 at 640.160 kb on + strand, within Shewana3_0544 at 640.583 kb on - strand at 640.603 kb on + strand at 640.628 kb on + strand at 640.636 kb on - strand at 640.636 kb on - strand at 640.656 kb on + strand at 640.688 kb on + strand at 640.721 kb on + strand at 640.890 kb on - strand at 641.188 kb on - strand, within Shewana3_0545 at 641.188 kb on - strand, within Shewana3_0545 at 641.236 kb on + strand, within Shewana3_0545 at 641.486 kb on + strand, within Shewana3_0545 at 641.526 kb on + strand, within Shewana3_0545 at 641.571 kb on + strand, within Shewana3_0545 at 641.579 kb on - strand, within Shewana3_0545 at 641.655 kb on + strand, within Shewana3_0545 at 642.001 kb on + strand, within Shewana3_0546 at 642.192 kb on + strand, within Shewana3_0546 at 642.216 kb on + strand, within Shewana3_0546 at 642.258 kb on - strand, within Shewana3_0546 at 642.260 kb on + strand, within Shewana3_0546 at 642.260 kb on + strand, within Shewana3_0546 at 642.266 kb on + strand, within Shewana3_0546 at 642.358 kb on + strand, within Shewana3_0546 at 642.522 kb on + strand at 642.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 640,114 + Shewana3_0544 0.67 +0.4 640,158 - Shewana3_0544 0.69 +0.2 640,160 + Shewana3_0544 0.70 +0.2 640,583 - -1.3 640,603 + -1.5 640,628 + -1.8 640,636 - -1.3 640,636 - -1.0 640,656 + +0.4 640,688 + +0.8 640,721 + -3.1 640,890 - +1.5 641,188 - Shewana3_0545 0.37 -1.2 641,188 - Shewana3_0545 0.37 +4.0 641,236 + Shewana3_0545 0.43 -0.3 641,486 + Shewana3_0545 0.70 +2.0 641,526 + Shewana3_0545 0.75 +4.4 641,571 + Shewana3_0545 0.80 +1.6 641,579 - Shewana3_0545 0.80 -1.6 641,655 + Shewana3_0545 0.89 -1.1 642,001 + Shewana3_0546 0.26 -0.4 642,192 + Shewana3_0546 0.54 +0.6 642,216 + Shewana3_0546 0.57 -1.5 642,258 - Shewana3_0546 0.63 +2.0 642,260 + Shewana3_0546 0.64 +0.5 642,260 + Shewana3_0546 0.64 -0.6 642,266 + Shewana3_0546 0.65 +0.5 642,358 + Shewana3_0546 0.78 -1.0 642,522 + +0.8 642,564 + +0.2
Or see this region's nucleotide sequence