Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0403 and frdB overlap by 1 nucleotides frdB and Shewana3_0405 are separated by 63 nucleotides Shewana3_0405 and Shewana3_0406 overlap by 4 nucleotides
Shewana3_0403: Shewana3_0403 - fumarate reductase flavoprotein subunit (RefSeq), at 461,958 to 463,964
_0403
Shewana3_0404: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 463,964 to 464,695
frdB
Shewana3_0405: Shewana3_0405 - antibiotic biosynthesis monooxygenase (RefSeq), at 464,759 to 465,043
_0405
Shewana3_0406: Shewana3_0406 - alcohol dehydrogenase (RefSeq), at 465,040 to 466,062
_0406
Position (kb) 464
465
466 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 463.775 kb on + strand at 464.103 kb on - strand, within frdB at 464.375 kb on - strand, within frdB at 464.452 kb on - strand, within frdB at 464.479 kb on - strand, within frdB at 464.539 kb on + strand, within frdB at 464.573 kb on + strand, within frdB at 464.578 kb on + strand, within frdB at 464.773 kb on - strand at 464.792 kb on + strand, within Shewana3_0405 at 464.800 kb on - strand, within Shewana3_0405 at 464.810 kb on - strand, within Shewana3_0405 at 464.905 kb on + strand, within Shewana3_0405 at 464.913 kb on + strand, within Shewana3_0405 at 464.937 kb on - strand, within Shewana3_0405 at 464.958 kb on + strand, within Shewana3_0405 at 464.966 kb on - strand, within Shewana3_0405 at 464.971 kb on + strand, within Shewana3_0405 at 464.979 kb on - strand, within Shewana3_0405 at 464.979 kb on - strand, within Shewana3_0405 at 464.991 kb on + strand, within Shewana3_0405 at 465.046 kb on + strand at 465.131 kb on + strand at 465.131 kb on + strand at 465.141 kb on - strand at 465.289 kb on - strand, within Shewana3_0406 at 465.344 kb on + strand, within Shewana3_0406 at 465.395 kb on - strand, within Shewana3_0406 at 465.397 kb on + strand, within Shewana3_0406 at 465.573 kb on + strand, within Shewana3_0406 at 465.692 kb on + strand, within Shewana3_0406 at 465.708 kb on + strand, within Shewana3_0406 at 465.853 kb on - strand, within Shewana3_0406 at 465.905 kb on - strand, within Shewana3_0406 at 465.955 kb on - strand, within Shewana3_0406 at 465.974 kb on - strand at 465.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 463,775 + -0.1 464,103 - frdB Shewana3_0404 0.19 +2.1 464,375 - frdB Shewana3_0404 0.56 +0.5 464,452 - frdB Shewana3_0404 0.67 +2.1 464,479 - frdB Shewana3_0404 0.70 +0.3 464,539 + frdB Shewana3_0404 0.79 -0.2 464,573 + frdB Shewana3_0404 0.83 -1.2 464,578 + frdB Shewana3_0404 0.84 -1.4 464,773 - -1.7 464,792 + Shewana3_0405 0.12 -0.3 464,800 - Shewana3_0405 0.14 -0.7 464,810 - Shewana3_0405 0.18 +2.0 464,905 + Shewana3_0405 0.51 -1.2 464,913 + Shewana3_0405 0.54 -1.4 464,937 - Shewana3_0405 0.62 +0.3 464,958 + Shewana3_0405 0.70 +0.4 464,966 - Shewana3_0405 0.73 -1.0 464,971 + Shewana3_0405 0.74 -1.4 464,979 - Shewana3_0405 0.77 +0.1 464,979 - Shewana3_0405 0.77 +0.3 464,991 + Shewana3_0405 0.81 -0.8 465,046 + +0.7 465,131 + -0.8 465,131 + +2.0 465,141 - +1.0 465,289 - Shewana3_0406 0.24 -2.2 465,344 + Shewana3_0406 0.30 +0.4 465,395 - Shewana3_0406 0.35 -1.5 465,397 + Shewana3_0406 0.35 +3.6 465,573 + Shewana3_0406 0.52 +1.0 465,692 + Shewana3_0406 0.64 -1.5 465,708 + Shewana3_0406 0.65 +0.0 465,853 - Shewana3_0406 0.79 +0.5 465,905 - Shewana3_0406 0.85 -0.1 465,955 - Shewana3_0406 0.89 -1.5 465,974 - -0.0 465,974 - +2.0
Or see this region's nucleotide sequence