Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0401 and Shewana3_0402 are separated by 206 nucleotides Shewana3_0402 and Shewana3_0403 are separated by 61 nucleotides Shewana3_0403 and frdB overlap by 1 nucleotides frdB and Shewana3_0405 are separated by 63 nucleotides
Shewana3_0401: Shewana3_0401 - fumarate reductase cytochrome b-556 subunit (RefSeq), at 460,293 to 461,018
_0401
Shewana3_0402: Shewana3_0402 - fumarate reductase respiratory complex, transmembrane subunit (RefSeq), at 461,225 to 461,896
_0402
Shewana3_0403: Shewana3_0403 - fumarate reductase flavoprotein subunit (RefSeq), at 461,958 to 463,964
_0403
Shewana3_0404: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 463,964 to 464,695
frdB
Shewana3_0405: Shewana3_0405 - antibiotic biosynthesis monooxygenase (RefSeq), at 464,759 to 465,043
_0405
Position (kb) 461
462
463
464 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 460.960 kb on + strand at 460.983 kb on - strand at 460.995 kb on + strand at 461.037 kb on + strand at 461.200 kb on - strand at 461.200 kb on - strand at 461.200 kb on - strand at 461.365 kb on - strand, within Shewana3_0402 at 461.370 kb on - strand, within Shewana3_0402 at 461.387 kb on - strand, within Shewana3_0402 at 461.418 kb on + strand, within Shewana3_0402 at 461.470 kb on - strand, within Shewana3_0402 at 461.535 kb on + strand, within Shewana3_0402 at 461.566 kb on + strand, within Shewana3_0402 at 461.571 kb on + strand, within Shewana3_0402 at 461.670 kb on + strand, within Shewana3_0402 at 461.765 kb on + strand, within Shewana3_0402 at 461.765 kb on + strand, within Shewana3_0402 at 461.942 kb on + strand at 462.015 kb on - strand at 462.025 kb on + strand at 462.103 kb on + strand at 462.237 kb on + strand, within Shewana3_0403 at 462.505 kb on + strand, within Shewana3_0403 at 462.550 kb on + strand, within Shewana3_0403 at 462.558 kb on - strand, within Shewana3_0403 at 462.630 kb on + strand, within Shewana3_0403 at 462.657 kb on + strand, within Shewana3_0403 at 462.657 kb on + strand, within Shewana3_0403 at 462.663 kb on + strand, within Shewana3_0403 at 462.664 kb on - strand, within Shewana3_0403 at 462.699 kb on - strand, within Shewana3_0403 at 462.707 kb on + strand, within Shewana3_0403 at 462.797 kb on + strand, within Shewana3_0403 at 462.953 kb on + strand, within Shewana3_0403 at 463.026 kb on - strand, within Shewana3_0403 at 463.112 kb on + strand, within Shewana3_0403 at 463.127 kb on - strand, within Shewana3_0403 at 463.147 kb on + strand, within Shewana3_0403 at 463.348 kb on + strand, within Shewana3_0403 at 463.411 kb on + strand, within Shewana3_0403 at 463.435 kb on - strand, within Shewana3_0403 at 463.518 kb on + strand, within Shewana3_0403 at 463.538 kb on + strand, within Shewana3_0403 at 463.596 kb on + strand, within Shewana3_0403 at 463.775 kb on + strand at 464.103 kb on - strand, within frdB at 464.375 kb on - strand, within frdB at 464.452 kb on - strand, within frdB at 464.479 kb on - strand, within frdB at 464.539 kb on + strand, within frdB at 464.573 kb on + strand, within frdB at 464.578 kb on + strand, within frdB at 464.773 kb on - strand at 464.792 kb on + strand, within Shewana3_0405 at 464.800 kb on - strand, within Shewana3_0405 at 464.810 kb on - strand, within Shewana3_0405 at 464.905 kb on + strand, within Shewana3_0405 at 464.913 kb on + strand, within Shewana3_0405 at 464.937 kb on - strand, within Shewana3_0405 at 464.958 kb on + strand, within Shewana3_0405
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 460,960 + +0.6 460,983 - +2.3 460,995 + -1.5 461,037 + +0.1 461,200 - +0.3 461,200 - +1.2 461,200 - -1.0 461,365 - Shewana3_0402 0.21 -2.0 461,370 - Shewana3_0402 0.22 -1.4 461,387 - Shewana3_0402 0.24 +0.3 461,418 + Shewana3_0402 0.29 +0.6 461,470 - Shewana3_0402 0.36 +0.8 461,535 + Shewana3_0402 0.46 -2.9 461,566 + Shewana3_0402 0.51 -1.4 461,571 + Shewana3_0402 0.51 -0.6 461,670 + Shewana3_0402 0.66 +0.4 461,765 + Shewana3_0402 0.80 -0.2 461,765 + Shewana3_0402 0.80 -1.1 461,942 + +0.7 462,015 - +0.6 462,025 + -1.0 462,103 + +0.2 462,237 + Shewana3_0403 0.14 -1.6 462,505 + Shewana3_0403 0.27 -1.0 462,550 + Shewana3_0403 0.29 -0.9 462,558 - Shewana3_0403 0.30 -0.9 462,630 + Shewana3_0403 0.33 -0.2 462,657 + Shewana3_0403 0.35 +0.2 462,657 + Shewana3_0403 0.35 +1.6 462,663 + Shewana3_0403 0.35 +0.4 462,664 - Shewana3_0403 0.35 -1.2 462,699 - Shewana3_0403 0.37 +1.6 462,707 + Shewana3_0403 0.37 -1.0 462,797 + Shewana3_0403 0.42 +0.1 462,953 + Shewana3_0403 0.50 -1.0 463,026 - Shewana3_0403 0.53 -0.3 463,112 + Shewana3_0403 0.57 +0.3 463,127 - Shewana3_0403 0.58 -1.2 463,147 + Shewana3_0403 0.59 +0.3 463,348 + Shewana3_0403 0.69 -2.4 463,411 + Shewana3_0403 0.72 -1.7 463,435 - Shewana3_0403 0.74 -1.6 463,518 + Shewana3_0403 0.78 +0.3 463,538 + Shewana3_0403 0.79 +0.6 463,596 + Shewana3_0403 0.82 +0.2 463,775 + -0.1 464,103 - frdB Shewana3_0404 0.19 +2.1 464,375 - frdB Shewana3_0404 0.56 +0.5 464,452 - frdB Shewana3_0404 0.67 +2.1 464,479 - frdB Shewana3_0404 0.70 +0.3 464,539 + frdB Shewana3_0404 0.79 -0.2 464,573 + frdB Shewana3_0404 0.83 -1.2 464,578 + frdB Shewana3_0404 0.84 -1.4 464,773 - -1.7 464,792 + Shewana3_0405 0.12 -0.3 464,800 - Shewana3_0405 0.14 -0.7 464,810 - Shewana3_0405 0.18 +2.0 464,905 + Shewana3_0405 0.51 -1.2 464,913 + Shewana3_0405 0.54 -1.4 464,937 - Shewana3_0405 0.62 +0.3 464,958 + Shewana3_0405 0.70 +0.4
Or see this region's nucleotide sequence