Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0166 and Shewana3_0167 are separated by 234 nucleotides Shewana3_0167 and Shewana3_0168 are separated by 119 nucleotides Shewana3_0168 and nudE are separated by 237 nucleotides nudE and Shewana3_0170 are separated by 70 nucleotides
Shewana3_0166: Shewana3_0166 - GCN5-related N-acetyltransferase (RefSeq), at 190,152 to 190,640
_0166
Shewana3_0167: Shewana3_0167 - phage tail collar domain-containing protein (RefSeq), at 190,875 to 191,510
_0167
Shewana3_0168: Shewana3_0168 - Ig family protein (RefSeq), at 191,630 to 202,264
_0168
Shewana3_0169: nudE - ADP-ribose diphosphatase NudE (RefSeq), at 202,502 to 203,053
nudE
Shewana3_0170: Shewana3_0170 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 203,124 to 203,936
_0170
Position (kb) 191
192
193
194
195
196
197
198
199
200
201
202
203 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 190.836 kb on + strand at 190.970 kb on + strand, within Shewana3_0167 at 191.034 kb on + strand, within Shewana3_0167 at 191.053 kb on + strand, within Shewana3_0167 at 191.244 kb on - strand, within Shewana3_0167 at 191.287 kb on - strand, within Shewana3_0167 at 191.365 kb on - strand, within Shewana3_0167 at 191.373 kb on + strand, within Shewana3_0167 at 191.373 kb on + strand, within Shewana3_0167 at 191.378 kb on + strand, within Shewana3_0167 at 191.381 kb on - strand, within Shewana3_0167 at 191.391 kb on - strand, within Shewana3_0167 at 191.407 kb on + strand, within Shewana3_0167 at 191.416 kb on - strand, within Shewana3_0167 at 191.422 kb on + strand, within Shewana3_0167 at 191.613 kb on + strand at 191.613 kb on + strand at 191.613 kb on + strand at 191.613 kb on + strand at 191.621 kb on - strand at 191.621 kb on - strand at 191.621 kb on - strand at 191.634 kb on - strand at 191.649 kb on - strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.762 kb on - strand at 191.892 kb on + strand at 191.899 kb on + strand at 191.942 kb on + strand at 191.979 kb on + strand at 192.047 kb on + strand at 192.093 kb on + strand at 192.117 kb on - strand at 192.242 kb on + strand at 192.268 kb on + strand at 192.304 kb on + strand at 192.463 kb on - strand at 192.622 kb on - strand at 192.660 kb on + strand at 192.747 kb on - strand, within Shewana3_0168 at 192.747 kb on - strand, within Shewana3_0168 at 192.806 kb on - strand, within Shewana3_0168 at 192.806 kb on - strand, within Shewana3_0168 at 192.910 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.013 kb on - strand, within Shewana3_0168 at 193.028 kb on + strand, within Shewana3_0168 at 193.044 kb on + strand, within Shewana3_0168 at 193.052 kb on - strand, within Shewana3_0168 at 193.052 kb on - strand, within Shewana3_0168 at 193.271 kb on + strand, within Shewana3_0168 at 193.327 kb on + strand, within Shewana3_0168 at 193.426 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.508 kb on + strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.516 kb on - strand, within Shewana3_0168 at 193.622 kb on + strand, within Shewana3_0168 at 193.630 kb on - strand, within Shewana3_0168 at 193.786 kb on - strand, within Shewana3_0168 at 193.838 kb on - strand, within Shewana3_0168 at 193.965 kb on - strand, within Shewana3_0168 at 194.115 kb on + strand, within Shewana3_0168 at 194.123 kb on - strand, within Shewana3_0168 at 194.161 kb on + strand, within Shewana3_0168 at 194.274 kb on + strand, within Shewana3_0168 at 194.462 kb on - strand, within Shewana3_0168 at 194.468 kb on - strand, within Shewana3_0168 at 194.783 kb on + strand, within Shewana3_0168 at 194.797 kb on + strand, within Shewana3_0168 at 195.063 kb on - strand, within Shewana3_0168 at 195.098 kb on + strand, within Shewana3_0168 at 195.106 kb on - strand, within Shewana3_0168 at 195.106 kb on - strand, within Shewana3_0168 at 195.228 kb on - strand, within Shewana3_0168 at 195.248 kb on - strand, within Shewana3_0168 at 195.271 kb on - strand, within Shewana3_0168 at 198.747 kb on + strand, within Shewana3_0168 at 198.787 kb on + strand, within Shewana3_0168 at 198.789 kb on + strand, within Shewana3_0168 at 198.811 kb on - strand, within Shewana3_0168 at 198.828 kb on + strand, within Shewana3_0168 at 198.836 kb on - strand, within Shewana3_0168 at 198.894 kb on + strand, within Shewana3_0168 at 199.097 kb on + strand, within Shewana3_0168 at 199.097 kb on + strand, within Shewana3_0168 at 199.105 kb on - strand, within Shewana3_0168 at 199.112 kb on - strand, within Shewana3_0168 at 199.137 kb on + strand, within Shewana3_0168 at 199.173 kb on - strand, within Shewana3_0168 at 199.300 kb on + strand, within Shewana3_0168 at 199.302 kb on + strand, within Shewana3_0168 at 199.307 kb on + strand, within Shewana3_0168 at 199.332 kb on + strand, within Shewana3_0168 at 199.332 kb on + strand, within Shewana3_0168 at 199.338 kb on - strand, within Shewana3_0168 at 199.338 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.340 kb on - strand, within Shewana3_0168 at 199.370 kb on + strand, within Shewana3_0168 at 199.441 kb on + strand, within Shewana3_0168 at 199.451 kb on - strand, within Shewana3_0168 at 199.451 kb on - strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.483 kb on + strand, within Shewana3_0168 at 199.491 kb on - strand, within Shewana3_0168 at 199.540 kb on + strand, within Shewana3_0168 at 199.548 kb on - strand, within Shewana3_0168 at 199.725 kb on - strand, within Shewana3_0168 at 199.838 kb on - strand, within Shewana3_0168 at 199.953 kb on + strand, within Shewana3_0168 at 199.959 kb on + strand, within Shewana3_0168 at 199.998 kb on + strand, within Shewana3_0168 at 200.035 kb on - strand, within Shewana3_0168 at 200.133 kb on + strand, within Shewana3_0168 at 200.172 kb on + strand, within Shewana3_0168 at 200.177 kb on + strand, within Shewana3_0168 at 200.210 kb on + strand, within Shewana3_0168 at 200.380 kb on - strand, within Shewana3_0168 at 200.597 kb on + strand, within Shewana3_0168 at 200.604 kb on + strand, within Shewana3_0168 at 200.714 kb on + strand, within Shewana3_0168 at 200.721 kb on + strand, within Shewana3_0168 at 200.867 kb on + strand, within Shewana3_0168 at 200.994 kb on + strand, within Shewana3_0168 at 201.015 kb on - strand, within Shewana3_0168 at 201.153 kb on - strand, within Shewana3_0168 at 201.155 kb on + strand, within Shewana3_0168 at 201.194 kb on + strand, within Shewana3_0168 at 201.269 kb on - strand at 201.605 kb on + strand at 201.628 kb on - strand at 201.641 kb on + strand at 201.704 kb on - strand at 201.813 kb on - strand at 201.831 kb on - strand at 201.844 kb on + strand at 201.999 kb on + strand at 202.027 kb on + strand at 202.118 kb on + strand at 202.187 kb on + strand at 202.470 kb on - strand at 202.602 kb on - strand, within nudE at 202.803 kb on + strand, within nudE at 202.818 kb on + strand, within nudE at 202.818 kb on + strand, within nudE at 202.887 kb on + strand, within nudE at 202.978 kb on - strand, within nudE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 190,836 + -1.1 190,970 + Shewana3_0167 0.15 +0.4 191,034 + Shewana3_0167 0.25 -4.4 191,053 + Shewana3_0167 0.28 +1.3 191,244 - Shewana3_0167 0.58 -0.8 191,287 - Shewana3_0167 0.65 +0.4 191,365 - Shewana3_0167 0.77 +0.4 191,373 + Shewana3_0167 0.78 +0.4 191,373 + Shewana3_0167 0.78 +0.0 191,378 + Shewana3_0167 0.79 +0.0 191,381 - Shewana3_0167 0.80 -0.6 191,391 - Shewana3_0167 0.81 +0.7 191,407 + Shewana3_0167 0.84 -0.0 191,416 - Shewana3_0167 0.85 +2.0 191,422 + Shewana3_0167 0.86 +0.6 191,613 + +0.4 191,613 + -1.7 191,613 + -0.7 191,613 + +0.6 191,621 - +0.4 191,621 - +1.4 191,621 - -0.1 191,634 - +0.4 191,649 - +1.7 191,754 + -0.9 191,754 + +0.4 191,762 - +0.1 191,892 + -1.8 191,899 + -1.6 191,942 + +0.5 191,979 + -1.6 192,047 + -1.3 192,093 + -0.4 192,117 - +0.4 192,242 + +0.7 192,268 + -2.2 192,304 + +0.2 192,463 - +0.2 192,622 - +0.5 192,660 + +0.5 192,747 - Shewana3_0168 0.11 +2.0 192,747 - Shewana3_0168 0.11 -1.1 192,806 - Shewana3_0168 0.11 -1.0 192,806 - Shewana3_0168 0.11 +0.7 192,910 - Shewana3_0168 0.12 -0.5 193,013 - Shewana3_0168 0.13 -1.5 193,013 - Shewana3_0168 0.13 +0.7 193,013 - Shewana3_0168 0.13 +0.1 193,013 - Shewana3_0168 0.13 +0.4 193,028 + Shewana3_0168 0.13 +2.3 193,044 + Shewana3_0168 0.13 -1.0 193,052 - Shewana3_0168 0.13 +0.1 193,052 - Shewana3_0168 0.13 -0.8 193,271 + Shewana3_0168 0.15 -0.3 193,327 + Shewana3_0168 0.16 +0.2 193,426 + Shewana3_0168 0.17 +0.6 193,508 + Shewana3_0168 0.18 -1.2 193,508 + Shewana3_0168 0.18 -0.3 193,508 + Shewana3_0168 0.18 -1.6 193,508 + Shewana3_0168 0.18 -1.5 193,508 + Shewana3_0168 0.18 +0.5 193,516 - Shewana3_0168 0.18 -1.6 193,516 - Shewana3_0168 0.18 +0.9 193,516 - Shewana3_0168 0.18 +0.2 193,516 - Shewana3_0168 0.18 +0.1 193,516 - Shewana3_0168 0.18 -0.8 193,622 + Shewana3_0168 0.19 +0.0 193,630 - Shewana3_0168 0.19 -1.1 193,786 - Shewana3_0168 0.20 -0.1 193,838 - Shewana3_0168 0.21 +2.4 193,965 - Shewana3_0168 0.22 +0.4 194,115 + Shewana3_0168 0.23 -1.0 194,123 - Shewana3_0168 0.23 -1.6 194,161 + Shewana3_0168 0.24 +0.5 194,274 + Shewana3_0168 0.25 -1.4 194,462 - Shewana3_0168 0.27 +0.2 194,468 - Shewana3_0168 0.27 -1.1 194,783 + Shewana3_0168 0.30 +0.3 194,797 + Shewana3_0168 0.30 -0.8 195,063 - Shewana3_0168 0.32 +0.5 195,098 + Shewana3_0168 0.33 +2.2 195,106 - Shewana3_0168 0.33 +0.2 195,106 - Shewana3_0168 0.33 -1.2 195,228 - Shewana3_0168 0.34 -0.9 195,248 - Shewana3_0168 0.34 +1.0 195,271 - Shewana3_0168 0.34 +0.4 198,747 + Shewana3_0168 0.67 -0.4 198,787 + Shewana3_0168 0.67 -1.4 198,789 + Shewana3_0168 0.67 -0.6 198,811 - Shewana3_0168 0.68 +0.4 198,828 + Shewana3_0168 0.68 +0.2 198,836 - Shewana3_0168 0.68 -1.2 198,894 + Shewana3_0168 0.68 +0.1 199,097 + Shewana3_0168 0.70 -1.4 199,097 + Shewana3_0168 0.70 +0.8 199,105 - Shewana3_0168 0.70 -1.1 199,112 - Shewana3_0168 0.70 -1.3 199,137 + Shewana3_0168 0.71 -1.5 199,173 - Shewana3_0168 0.71 +1.1 199,300 + Shewana3_0168 0.72 +1.1 199,302 + Shewana3_0168 0.72 +0.7 199,307 + Shewana3_0168 0.72 -0.9 199,332 + Shewana3_0168 0.72 +0.2 199,332 + Shewana3_0168 0.72 +0.6 199,338 - Shewana3_0168 0.72 -1.7 199,338 - Shewana3_0168 0.72 +0.7 199,340 - Shewana3_0168 0.72 +1.7 199,340 - Shewana3_0168 0.72 -0.6 199,340 - Shewana3_0168 0.72 +0.4 199,340 - Shewana3_0168 0.72 +0.9 199,370 + Shewana3_0168 0.73 +2.9 199,441 + Shewana3_0168 0.73 +0.7 199,451 - Shewana3_0168 0.74 +1.1 199,451 - Shewana3_0168 0.74 -0.9 199,483 + Shewana3_0168 0.74 -1.3 199,483 + Shewana3_0168 0.74 -0.7 199,483 + Shewana3_0168 0.74 +1.6 199,483 + Shewana3_0168 0.74 +0.1 199,491 - Shewana3_0168 0.74 -1.0 199,540 + Shewana3_0168 0.74 -1.3 199,548 - Shewana3_0168 0.74 +0.7 199,725 - Shewana3_0168 0.76 -1.5 199,838 - Shewana3_0168 0.77 +2.7 199,953 + Shewana3_0168 0.78 +0.1 199,959 + Shewana3_0168 0.78 +0.7 199,998 + Shewana3_0168 0.79 +0.7 200,035 - Shewana3_0168 0.79 +0.4 200,133 + Shewana3_0168 0.80 +0.3 200,172 + Shewana3_0168 0.80 +0.5 200,177 + Shewana3_0168 0.80 -1.8 200,210 + Shewana3_0168 0.81 -1.1 200,380 - Shewana3_0168 0.82 -1.3 200,597 + Shewana3_0168 0.84 +0.1 200,604 + Shewana3_0168 0.84 +0.5 200,714 + Shewana3_0168 0.85 -1.3 200,721 + Shewana3_0168 0.85 -0.5 200,867 + Shewana3_0168 0.87 -1.3 200,994 + Shewana3_0168 0.88 -1.3 201,015 - Shewana3_0168 0.88 -1.3 201,153 - Shewana3_0168 0.90 -0.7 201,155 + Shewana3_0168 0.90 -1.2 201,194 + Shewana3_0168 0.90 -1.1 201,269 - +2.1 201,605 + -0.9 201,628 - +0.6 201,641 + +0.7 201,704 - +0.7 201,813 - -0.0 201,831 - -1.9 201,844 + -0.1 201,999 + +2.3 202,027 + -0.8 202,118 + +0.2 202,187 + +0.9 202,470 - +0.2 202,602 - nudE Shewana3_0169 0.18 -0.1 202,803 + nudE Shewana3_0169 0.55 -1.9 202,818 + nudE Shewana3_0169 0.57 -1.1 202,818 + nudE Shewana3_0169 0.57 -0.7 202,887 + nudE Shewana3_0169 0.70 -0.4 202,978 - nudE Shewana3_0169 0.86 -2.2
Or see this region's nucleotide sequence