Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4142 and Shewana3_4143 are separated by 83 nucleotides Shewana3_4143 and Shewana3_4144 are separated by 43 nucleotides Shewana3_4144 and Shewana3_4145 are separated by 36 nucleotides
Shewana3_4142: Shewana3_4142 - IS4 family transposase (RefSeq), at 1,491 to 2,471
_4142
Shewana3_4143: Shewana3_4143 - hypothetical protein (RefSeq), at 2,555 to 2,836
_4143
Shewana3_4144: Shewana3_4144 - plastocyanin domain-containing protein (RefSeq), at 2,880 to 3,239
_4144
Shewana3_4145: Shewana3_4145 - copper-translocating P-type ATPase (RefSeq), at 3,276 to 5,612
_4145
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1.980 kb on - strand, within Shewana3_4142 at 2.130 kb on - strand, within Shewana3_4142 at 2.130 kb on - strand, within Shewana3_4142 at 2.181 kb on - strand, within Shewana3_4142 at 2.252 kb on + strand, within Shewana3_4142 at 2.276 kb on - strand, within Shewana3_4142 at 2.337 kb on - strand, within Shewana3_4142 at 2.352 kb on - strand, within Shewana3_4142 at 2.545 kb on - strand at 2.898 kb on + strand at 2.903 kb on + strand at 2.906 kb on - strand at 3.106 kb on + strand, within Shewana3_4144 at 3.209 kb on + strand at 3.231 kb on - strand at 3.321 kb on - strand at 3.486 kb on - strand at 3.501 kb on + strand at 3.828 kb on - strand, within Shewana3_4145 at 4.013 kb on + strand, within Shewana3_4145
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 1,980 - Shewana3_4142 0.50 -0.9 2,130 - Shewana3_4142 0.65 +1.3 2,130 - Shewana3_4142 0.65 +2.9 2,181 - Shewana3_4142 0.70 -1.8 2,252 + Shewana3_4142 0.78 +0.2 2,276 - Shewana3_4142 0.80 +0.7 2,337 - Shewana3_4142 0.86 -2.9 2,352 - Shewana3_4142 0.88 +2.4 2,545 - +0.2 2,898 + +1.3 2,903 + -2.1 2,906 - -1.7 3,106 + Shewana3_4144 0.63 -1.4 3,209 + +0.9 3,231 - -0.4 3,321 - -1.4 3,486 - +2.7 3,501 + -1.4 3,828 - Shewana3_4145 0.24 -1.5 4,013 + Shewana3_4145 0.32 -1.0
Or see this region's nucleotide sequence