Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoL and nuoK overlap by 4 nucleotides nuoK and nuoJ overlap by 4 nucleotides nuoJ and nuoI are separated by 10 nucleotides nuoI and nuoH are separated by 8 nucleotides
SO1011: nuoL - NADH dehydrogenase I, L subunit (NCBI ptt file), at 1,048,384 to 1,050,231
nuoL
SO1012: nuoK - NADH dehydrogenase I, K subunit (NCBI ptt file), at 1,050,228 to 1,050,536
nuoK
SO1013: nuoJ - NADH dehydrogenase I, J subunit (NCBI ptt file), at 1,050,533 to 1,051,084
nuoJ
SO1014: nuoI - NADH dehydrogenase I, I subunit (NCBI ptt file), at 1,051,095 to 1,051,637
nuoI
SO1015: nuoH - NADH dehydrogenase I, H subunit (NCBI ptt file), at 1,051,646 to 1,052,614
nuoH
Position (kb)
1050
1051
1052 Strain fitness (log2 ratio)
-2
-1
0
1 at 1049.581 kb on - strand, within nuoL at 1049.581 kb on - strand, within nuoL at 1049.583 kb on + strand, within nuoL at 1049.583 kb on + strand, within nuoL at 1049.583 kb on + strand, within nuoL at 1049.591 kb on - strand, within nuoL at 1049.637 kb on + strand, within nuoL at 1049.652 kb on + strand, within nuoL at 1049.697 kb on - strand at 1049.725 kb on - strand, within nuoL at 1049.725 kb on - strand, within nuoL at 1049.748 kb on + strand, within nuoL at 1049.774 kb on + strand, within nuoL at 1049.792 kb on - strand, within nuoL at 1050.114 kb on + strand at 1050.114 kb on + strand at 1050.122 kb on - strand at 1050.133 kb on + strand at 1050.178 kb on - strand at 1050.183 kb on - strand at 1050.261 kb on - strand, within nuoK at 1050.285 kb on - strand, within nuoK at 1050.315 kb on - strand, within nuoK at 1050.371 kb on - strand, within nuoK at 1050.459 kb on + strand, within nuoK at 1050.467 kb on - strand, within nuoK at 1050.481 kb on + strand, within nuoK at 1050.481 kb on + strand, within nuoK at 1050.503 kb on + strand, within nuoK at 1050.503 kb on + strand, within nuoK at 1050.511 kb on - strand at 1050.543 kb on + strand at 1050.587 kb on - strand at 1050.589 kb on + strand, within nuoJ at 1050.605 kb on - strand, within nuoJ at 1050.696 kb on + strand, within nuoJ at 1050.706 kb on + strand, within nuoJ at 1050.706 kb on + strand, within nuoJ at 1050.714 kb on - strand, within nuoJ at 1051.006 kb on + strand, within nuoJ at 1051.155 kb on - strand, within nuoI at 1051.318 kb on + strand, within nuoI at 1051.446 kb on - strand, within nuoI at 1051.454 kb on - strand, within nuoI at 1051.485 kb on + strand, within nuoI at 1051.485 kb on + strand, within nuoI at 1051.493 kb on - strand, within nuoI at 1051.611 kb on + strand at 1051.677 kb on + strand at 1051.735 kb on - strand at 1051.746 kb on - strand, within nuoH at 1051.786 kb on + strand, within nuoH at 1051.786 kb on + strand, within nuoH at 1051.815 kb on - strand, within nuoH at 1051.869 kb on - strand, within nuoH at 1051.974 kb on + strand, within nuoH at 1051.996 kb on + strand, within nuoH at 1052.063 kb on + strand, within nuoH at 1052.063 kb on + strand, within nuoH at 1052.073 kb on + strand, within nuoH
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,049,581 - nuoL SO1011 0.65 -0.3 1,049,581 - nuoL SO1011 0.65 -0.4 1,049,583 + nuoL SO1011 0.65 -0.9 1,049,583 + nuoL SO1011 0.65 +0.7 1,049,583 + nuoL SO1011 0.65 -0.1 1,049,591 - nuoL SO1011 0.65 +0.1 1,049,637 + nuoL SO1011 0.68 -0.4 1,049,652 + nuoL SO1011 0.69 -1.8 1,049,697 - +1.2 1,049,725 - nuoL SO1011 0.73 -0.0 1,049,725 - nuoL SO1011 0.73 -0.1 1,049,748 + nuoL SO1011 0.74 -0.4 1,049,774 + nuoL SO1011 0.75 -0.2 1,049,792 - nuoL SO1011 0.76 -1.4 1,050,114 + -0.9 1,050,114 + +0.1 1,050,122 - -0.7 1,050,133 + -0.0 1,050,178 - -1.1 1,050,183 - -0.5 1,050,261 - nuoK SO1012 0.11 -1.6 1,050,285 - nuoK SO1012 0.18 -0.2 1,050,315 - nuoK SO1012 0.28 -0.3 1,050,371 - nuoK SO1012 0.46 -0.2 1,050,459 + nuoK SO1012 0.75 -1.1 1,050,467 - nuoK SO1012 0.77 +0.1 1,050,481 + nuoK SO1012 0.82 -0.3 1,050,481 + nuoK SO1012 0.82 -0.4 1,050,503 + nuoK SO1012 0.89 -0.2 1,050,503 + nuoK SO1012 0.89 -0.9 1,050,511 - -0.8 1,050,543 + -0.5 1,050,587 - +0.3 1,050,589 + nuoJ SO1013 0.10 -1.7 1,050,605 - nuoJ SO1013 0.13 -0.5 1,050,696 + nuoJ SO1013 0.30 -0.9 1,050,706 + nuoJ SO1013 0.31 -0.4 1,050,706 + nuoJ SO1013 0.31 +0.1 1,050,714 - nuoJ SO1013 0.33 -0.8 1,051,006 + nuoJ SO1013 0.86 -0.1 1,051,155 - nuoI SO1014 0.11 -0.2 1,051,318 + nuoI SO1014 0.41 -0.5 1,051,446 - nuoI SO1014 0.65 +0.3 1,051,454 - nuoI SO1014 0.66 -0.2 1,051,485 + nuoI SO1014 0.72 -1.5 1,051,485 + nuoI SO1014 0.72 -0.5 1,051,493 - nuoI SO1014 0.73 +0.2 1,051,611 + -0.5 1,051,677 + -1.5 1,051,735 - -0.8 1,051,746 - nuoH SO1015 0.10 -0.1 1,051,786 + nuoH SO1015 0.14 +1.1 1,051,786 + nuoH SO1015 0.14 +0.2 1,051,815 - nuoH SO1015 0.17 -0.2 1,051,869 - nuoH SO1015 0.23 -0.2 1,051,974 + nuoH SO1015 0.34 +0.3 1,051,996 + nuoH SO1015 0.36 -0.8 1,052,063 + nuoH SO1015 0.43 -0.2 1,052,063 + nuoH SO1015 0.43 -1.1 1,052,073 + nuoH SO1015 0.44 -0.3
Or see this region's nucleotide sequence