Experiment: Tween 20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_2292 and Sama_2293 are separated by 116 nucleotides Sama_2293 and fliI are separated by 3 nucleotides fliI and fliH overlap by 32 nucleotides fliH and fliG overlap by 1 nucleotides
Sama_2292: Sama_2292 - flagellar hook-length control protein FliK (RefSeq), at 2,756,429 to 2,758,210
_2292
Sama_2293: Sama_2293 - flagellar protein FliJ (RefSeq), at 2,758,327 to 2,758,773
_2293
Sama_2294: fliI - flagellum-specific ATP synthase (RefSeq), at 2,758,777 to 2,760,117
fliI
Sama_2295: fliH - flagellar assembly protein H (RefSeq), at 2,760,086 to 2,760,988
fliH
Sama_2296: fliG - flagellar motor switch protein G (RefSeq), at 2,760,988 to 2,762,025
fliG
Position (kb)
2758
2759
2760
2761 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2757.917 kb on + strand, within Sama_2292 at 2758.066 kb on + strand at 2758.233 kb on + strand at 2758.233 kb on + strand at 2758.233 kb on + strand at 2758.233 kb on + strand at 2758.233 kb on + strand at 2758.234 kb on - strand at 2758.234 kb on - strand at 2758.234 kb on - strand at 2758.240 kb on - strand at 2758.286 kb on + strand at 2758.319 kb on + strand at 2758.328 kb on + strand at 2758.328 kb on + strand at 2758.329 kb on - strand at 2758.329 kb on - strand at 2758.429 kb on - strand, within Sama_2293 at 2758.534 kb on + strand, within Sama_2293 at 2758.534 kb on + strand, within Sama_2293 at 2758.534 kb on + strand, within Sama_2293 at 2758.557 kb on + strand, within Sama_2293 at 2758.557 kb on + strand, within Sama_2293 at 2758.577 kb on + strand, within Sama_2293 at 2758.578 kb on - strand, within Sama_2293 at 2758.578 kb on - strand, within Sama_2293 at 2758.580 kb on + strand, within Sama_2293 at 2758.581 kb on - strand, within Sama_2293 at 2758.626 kb on + strand, within Sama_2293 at 2758.626 kb on + strand, within Sama_2293 at 2758.626 kb on + strand, within Sama_2293 at 2758.626 kb on + strand, within Sama_2293 at 2758.627 kb on - strand, within Sama_2293 at 2758.627 kb on - strand, within Sama_2293 at 2758.627 kb on - strand, within Sama_2293 at 2758.627 kb on - strand, within Sama_2293 at 2758.627 kb on - strand, within Sama_2293 at 2758.627 kb on - strand, within Sama_2293 at 2758.628 kb on + strand, within Sama_2293 at 2758.628 kb on + strand, within Sama_2293 at 2758.628 kb on + strand, within Sama_2293 at 2758.629 kb on - strand, within Sama_2293 at 2758.779 kb on - strand at 2758.785 kb on + strand at 2758.786 kb on - strand at 2758.786 kb on - strand at 2758.786 kb on - strand at 2758.902 kb on + strand at 2758.902 kb on + strand at 2758.926 kb on - strand, within fliI at 2758.961 kb on + strand, within fliI at 2758.970 kb on + strand, within fliI at 2758.970 kb on + strand, within fliI at 2758.971 kb on - strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.101 kb on + strand, within fliI at 2759.102 kb on - strand, within fliI at 2759.102 kb on - strand, within fliI at 2759.102 kb on - strand, within fliI at 2759.170 kb on + strand, within fliI at 2759.171 kb on - strand, within fliI at 2759.171 kb on - strand, within fliI at 2759.171 kb on - strand, within fliI at 2759.264 kb on + strand, within fliI at 2759.338 kb on - strand, within fliI at 2759.338 kb on - strand, within fliI at 2759.343 kb on + strand, within fliI at 2759.344 kb on - strand, within fliI at 2759.344 kb on - strand, within fliI at 2759.358 kb on + strand, within fliI at 2759.358 kb on + strand, within fliI at 2759.358 kb on + strand, within fliI at 2759.358 kb on + strand, within fliI at 2759.359 kb on - strand, within fliI at 2759.359 kb on - strand, within fliI at 2759.359 kb on - strand, within fliI at 2759.359 kb on - strand, within fliI at 2759.378 kb on + strand, within fliI at 2759.395 kb on - strand, within fliI at 2759.497 kb on - strand, within fliI at 2759.592 kb on + strand, within fliI at 2759.753 kb on - strand, within fliI at 2759.803 kb on + strand, within fliI at 2759.804 kb on - strand, within fliI at 2759.804 kb on - strand, within fliI at 2759.878 kb on + strand, within fliI at 2759.879 kb on - strand, within fliI at 2759.879 kb on - strand, within fliI at 2759.879 kb on - strand, within fliI at 2759.879 kb on - strand, within fliI at 2759.879 kb on - strand, within fliI at 2759.900 kb on + strand, within fliI at 2759.901 kb on - strand, within fliI at 2759.901 kb on - strand, within fliI at 2759.901 kb on - strand, within fliI at 2759.937 kb on + strand, within fliI at 2760.088 kb on - strand at 2760.112 kb on + strand at 2760.113 kb on - strand at 2760.113 kb on - strand at 2760.113 kb on - strand at 2760.197 kb on + strand, within fliH at 2760.197 kb on + strand, within fliH at 2760.197 kb on + strand, within fliH at 2760.198 kb on - strand, within fliH at 2760.249 kb on - strand, within fliH at 2760.249 kb on - strand, within fliH at 2760.278 kb on - strand, within fliH at 2760.356 kb on - strand, within fliH at 2760.378 kb on + strand, within fliH at 2760.378 kb on + strand, within fliH at 2760.379 kb on - strand, within fliH at 2760.379 kb on - strand, within fliH at 2760.480 kb on - strand, within fliH at 2760.480 kb on - strand, within fliH at 2760.713 kb on + strand, within fliH at 2760.714 kb on - strand, within fliH at 2760.801 kb on - strand, within fliH at 2760.801 kb on - strand, within fliH at 2760.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 remove 2,757,917 + Sama_2292 0.84 -1.2 2,758,066 + -1.9 2,758,233 + -1.8 2,758,233 + -1.4 2,758,233 + -0.9 2,758,233 + +0.3 2,758,233 + -1.3 2,758,234 - +0.4 2,758,234 - -0.1 2,758,234 - +0.1 2,758,240 - +0.4 2,758,286 + +0.8 2,758,319 + +1.6 2,758,328 + +0.6 2,758,328 + -0.0 2,758,329 - -0.5 2,758,329 - +1.6 2,758,429 - Sama_2293 0.23 +0.5 2,758,534 + Sama_2293 0.46 -3.4 2,758,534 + Sama_2293 0.46 +0.1 2,758,534 + Sama_2293 0.46 -1.7 2,758,557 + Sama_2293 0.51 -0.1 2,758,557 + Sama_2293 0.51 -3.7 2,758,577 + Sama_2293 0.56 -2.7 2,758,578 - Sama_2293 0.56 -2.3 2,758,578 - Sama_2293 0.56 -1.8 2,758,580 + Sama_2293 0.57 -1.9 2,758,581 - Sama_2293 0.57 -1.1 2,758,626 + Sama_2293 0.67 -0.9 2,758,626 + Sama_2293 0.67 -2.1 2,758,626 + Sama_2293 0.67 +0.0 2,758,626 + Sama_2293 0.67 -1.9 2,758,627 - Sama_2293 0.67 -2.4 2,758,627 - Sama_2293 0.67 -0.8 2,758,627 - Sama_2293 0.67 -2.1 2,758,627 - Sama_2293 0.67 -1.4 2,758,627 - Sama_2293 0.67 -1.6 2,758,627 - Sama_2293 0.67 -1.1 2,758,628 + Sama_2293 0.67 -3.1 2,758,628 + Sama_2293 0.67 -3.4 2,758,628 + Sama_2293 0.67 -2.3 2,758,629 - Sama_2293 0.68 -0.1 2,758,779 - -1.0 2,758,785 + -1.4 2,758,786 - +0.3 2,758,786 - +1.4 2,758,786 - -2.8 2,758,902 + -0.4 2,758,902 + -1.1 2,758,926 - fliI Sama_2294 0.11 -3.9 2,758,961 + fliI Sama_2294 0.14 -2.2 2,758,970 + fliI Sama_2294 0.14 -1.6 2,758,970 + fliI Sama_2294 0.14 -1.1 2,758,971 - fliI Sama_2294 0.14 -1.1 2,759,101 + fliI Sama_2294 0.24 -2.4 2,759,101 + fliI Sama_2294 0.24 -2.1 2,759,101 + fliI Sama_2294 0.24 -1.4 2,759,101 + fliI Sama_2294 0.24 -3.9 2,759,101 + fliI Sama_2294 0.24 -1.8 2,759,101 + fliI Sama_2294 0.24 -1.4 2,759,101 + fliI Sama_2294 0.24 -2.1 2,759,101 + fliI Sama_2294 0.24 -1.1 2,759,102 - fliI Sama_2294 0.24 -0.8 2,759,102 - fliI Sama_2294 0.24 -0.5 2,759,102 - fliI Sama_2294 0.24 -0.7 2,759,170 + fliI Sama_2294 0.29 -1.7 2,759,171 - fliI Sama_2294 0.29 -1.1 2,759,171 - fliI Sama_2294 0.29 -3.7 2,759,171 - fliI Sama_2294 0.29 -0.7 2,759,264 + fliI Sama_2294 0.36 -1.6 2,759,338 - fliI Sama_2294 0.42 -2.6 2,759,338 - fliI Sama_2294 0.42 -0.7 2,759,343 + fliI Sama_2294 0.42 -1.7 2,759,344 - fliI Sama_2294 0.42 -1.8 2,759,344 - fliI Sama_2294 0.42 -2.7 2,759,358 + fliI Sama_2294 0.43 -0.1 2,759,358 + fliI Sama_2294 0.43 -2.3 2,759,358 + fliI Sama_2294 0.43 -2.7 2,759,358 + fliI Sama_2294 0.43 -3.0 2,759,359 - fliI Sama_2294 0.43 -3.7 2,759,359 - fliI Sama_2294 0.43 -2.1 2,759,359 - fliI Sama_2294 0.43 -2.8 2,759,359 - fliI Sama_2294 0.43 -2.8 2,759,378 + fliI Sama_2294 0.45 +0.1 2,759,395 - fliI Sama_2294 0.46 -0.1 2,759,497 - fliI Sama_2294 0.54 -1.9 2,759,592 + fliI Sama_2294 0.61 -2.2 2,759,753 - fliI Sama_2294 0.73 -1.9 2,759,803 + fliI Sama_2294 0.77 -2.1 2,759,804 - fliI Sama_2294 0.77 -2.1 2,759,804 - fliI Sama_2294 0.77 -1.4 2,759,878 + fliI Sama_2294 0.82 -2.9 2,759,879 - fliI Sama_2294 0.82 +0.7 2,759,879 - fliI Sama_2294 0.82 +0.9 2,759,879 - fliI Sama_2294 0.82 -2.1 2,759,879 - fliI Sama_2294 0.82 -1.1 2,759,879 - fliI Sama_2294 0.82 -2.4 2,759,900 + fliI Sama_2294 0.84 -1.9 2,759,901 - fliI Sama_2294 0.84 -0.7 2,759,901 - fliI Sama_2294 0.84 -1.9 2,759,901 - fliI Sama_2294 0.84 -0.1 2,759,937 + fliI Sama_2294 0.87 -2.3 2,760,088 - -2.1 2,760,112 + -0.7 2,760,113 - -2.1 2,760,113 - -3.2 2,760,113 - -1.4 2,760,197 + fliH Sama_2295 0.12 -1.7 2,760,197 + fliH Sama_2295 0.12 -0.7 2,760,197 + fliH Sama_2295 0.12 -1.1 2,760,198 - fliH Sama_2295 0.12 -1.4 2,760,249 - fliH Sama_2295 0.18 -0.8 2,760,249 - fliH Sama_2295 0.18 -0.8 2,760,278 - fliH Sama_2295 0.21 -3.1 2,760,356 - fliH Sama_2295 0.30 -1.7 2,760,378 + fliH Sama_2295 0.32 -1.1 2,760,378 + fliH Sama_2295 0.32 -1.9 2,760,379 - fliH Sama_2295 0.32 -2.7 2,760,379 - fliH Sama_2295 0.32 -2.1 2,760,480 - fliH Sama_2295 0.44 -2.6 2,760,480 - fliH Sama_2295 0.44 -1.1 2,760,713 + fliH Sama_2295 0.69 -3.2 2,760,714 - fliH Sama_2295 0.70 -2.6 2,760,801 - fliH Sama_2295 0.79 -1.9 2,760,801 - fliH Sama_2295 0.79 -2.1 2,760,959 + -0.3
Or see this region's nucleotide sequence