Experiment: L-Glutamic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_287 and HP15_288 are separated by 61 nucleotides HP15_288 and HP15_289 are separated by 224 nucleotides HP15_289 and HP15_290 are separated by 42 nucleotides
HP15_287: HP15_287 - pyrroline-5-carboxylate reductase, at 278,227 to 279,060
_287
HP15_288: HP15_288 - alanine racemase domain protein, at 279,122 to 279,742
_288
HP15_289: HP15_289 - twitching motility protein PilT, at 279,967 to 281,001
_289
HP15_290: HP15_290 - twitching motility protein PilU PilT, at 281,044 to 282,165
_290
Position (kb)
279
280
281
282 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 279.061 kb on + strand at 279.067 kb on + strand at 279.094 kb on + strand at 279.097 kb on - strand at 279.102 kb on - strand at 279.151 kb on + strand at 279.151 kb on + strand at 279.159 kb on - strand at 279.159 kb on - strand at 279.164 kb on - strand at 279.228 kb on + strand, within HP15_288 at 279.231 kb on - strand, within HP15_288 at 279.261 kb on - strand, within HP15_288 at 279.312 kb on + strand, within HP15_288 at 279.411 kb on - strand, within HP15_288 at 279.510 kb on + strand, within HP15_288 at 279.510 kb on + strand, within HP15_288 at 279.623 kb on + strand, within HP15_288 at 279.654 kb on - strand, within HP15_288 at 279.739 kb on + strand at 279.739 kb on + strand at 279.811 kb on + strand at 279.824 kb on - strand at 280.133 kb on + strand, within HP15_289 at 280.251 kb on + strand, within HP15_289 at 280.273 kb on + strand, within HP15_289 at 280.330 kb on - strand, within HP15_289 at 280.337 kb on + strand, within HP15_289 at 280.345 kb on - strand, within HP15_289 at 280.364 kb on + strand, within HP15_289 at 280.536 kb on - strand, within HP15_289 at 280.576 kb on - strand, within HP15_289 at 280.578 kb on + strand, within HP15_289 at 280.640 kb on - strand, within HP15_289 at 280.694 kb on - strand, within HP15_289 at 280.732 kb on - strand, within HP15_289 at 280.866 kb on + strand, within HP15_289 at 280.930 kb on - strand at 280.971 kb on + strand at 280.971 kb on + strand at 280.995 kb on + strand at 281.131 kb on - strand at 281.181 kb on + strand, within HP15_290 at 281.326 kb on + strand, within HP15_290 at 281.397 kb on + strand, within HP15_290 at 281.405 kb on - strand, within HP15_290 at 281.426 kb on - strand, within HP15_290 at 281.451 kb on + strand, within HP15_290 at 281.550 kb on + strand, within HP15_290 at 281.558 kb on - strand, within HP15_290 at 281.560 kb on - strand, within HP15_290 at 281.580 kb on + strand, within HP15_290 at 281.610 kb on + strand, within HP15_290 at 281.618 kb on - strand, within HP15_290 at 281.625 kb on - strand, within HP15_290 at 281.630 kb on + strand, within HP15_290 at 281.641 kb on + strand, within HP15_290 at 281.754 kb on + strand, within HP15_290 at 281.801 kb on + strand, within HP15_290 at 281.834 kb on + strand, within HP15_290 at 281.837 kb on - strand, within HP15_290 at 281.898 kb on - strand, within HP15_290 at 281.903 kb on - strand, within HP15_290 at 281.903 kb on - strand, within HP15_290 at 281.903 kb on - strand, within HP15_290 at 281.945 kb on + strand, within HP15_290 at 281.990 kb on + strand, within HP15_290
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic remove 279,061 + -1.3 279,067 + -1.3 279,094 + -1.0 279,097 - +1.4 279,102 - -0.6 279,151 + +1.1 279,151 + +1.4 279,159 - +0.2 279,159 - +0.3 279,164 - -0.9 279,228 + HP15_288 0.17 +0.3 279,231 - HP15_288 0.18 +1.9 279,261 - HP15_288 0.22 -1.6 279,312 + HP15_288 0.31 -1.4 279,411 - HP15_288 0.47 -0.6 279,510 + HP15_288 0.62 -0.4 279,510 + HP15_288 0.62 +1.0 279,623 + HP15_288 0.81 +1.2 279,654 - HP15_288 0.86 +1.5 279,739 + +1.7 279,739 + +0.9 279,811 + -0.8 279,824 - -1.3 280,133 + HP15_289 0.16 -1.7 280,251 + HP15_289 0.27 +1.1 280,273 + HP15_289 0.30 -1.9 280,330 - HP15_289 0.35 -0.7 280,337 + HP15_289 0.36 +1.0 280,345 - HP15_289 0.37 +1.3 280,364 + HP15_289 0.38 -3.2 280,536 - HP15_289 0.55 -2.0 280,576 - HP15_289 0.59 -2.1 280,578 + HP15_289 0.59 -3.1 280,640 - HP15_289 0.65 -2.5 280,694 - HP15_289 0.70 -1.5 280,732 - HP15_289 0.74 -0.8 280,866 + HP15_289 0.87 +1.2 280,930 - -1.3 280,971 + +0.1 280,971 + +0.7 280,995 + +1.6 281,131 - -0.6 281,181 + HP15_290 0.12 +1.2 281,326 + HP15_290 0.25 -0.1 281,397 + HP15_290 0.31 -0.4 281,405 - HP15_290 0.32 -0.5 281,426 - HP15_290 0.34 +1.9 281,451 + HP15_290 0.36 -0.4 281,550 + HP15_290 0.45 +1.4 281,558 - HP15_290 0.46 -0.1 281,560 - HP15_290 0.46 +2.0 281,580 + HP15_290 0.48 +0.0 281,610 + HP15_290 0.50 +1.9 281,618 - HP15_290 0.51 -1.1 281,625 - HP15_290 0.52 -0.5 281,630 + HP15_290 0.52 -1.0 281,641 + HP15_290 0.53 -0.9 281,754 + HP15_290 0.63 +1.3 281,801 + HP15_290 0.67 -0.6 281,834 + HP15_290 0.70 -0.4 281,837 - HP15_290 0.71 -0.5 281,898 - HP15_290 0.76 -0.9 281,903 - HP15_290 0.77 -0.2 281,903 - HP15_290 0.77 -1.1 281,903 - HP15_290 0.77 -0.3 281,945 + HP15_290 0.80 -0.1 281,990 + HP15_290 0.84 -0.2
Or see this region's nucleotide sequence