Experiment: L-Glutamic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_217 and HP15_218 are separated by 122 nucleotides HP15_218 and HP15_219 are separated by 20 nucleotides HP15_219 and HP15_220 are separated by 150 nucleotides
HP15_217: HP15_217 - glutamate--cysteine ligase, at 205,710 to 207,263
_217
HP15_218: HP15_218 - conserved hypothetical protein, at 207,386 to 207,601
_218
HP15_219: HP15_219 - flagellar basal body-associated protein FliL, at 207,622 to 208,071
_219
HP15_220: HP15_220 - disulfide bond formation protein B, at 208,222 to 208,629
_220
Position (kb) 207
208 Strain fitness (log2 ratio) -2
-1
0
1
2 at 207.277 kb on - strand at 207.427 kb on + strand, within HP15_218 at 207.455 kb on - strand, within HP15_218 at 207.560 kb on - strand, within HP15_218 at 208.110 kb on + strand at 208.282 kb on - strand, within HP15_220 at 208.308 kb on + strand, within HP15_220 at 208.444 kb on - strand, within HP15_220 at 208.486 kb on - strand, within HP15_220 at 208.505 kb on - strand, within HP15_220 at 208.564 kb on + strand, within HP15_220 at 208.572 kb on - strand, within HP15_220
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic remove 207,277 - +2.0 207,427 + HP15_218 0.19 +0.7 207,455 - HP15_218 0.32 +2.0 207,560 - HP15_218 0.81 +2.3 208,110 + +0.8 208,282 - HP15_220 0.15 -0.4 208,308 + HP15_220 0.21 +0.3 208,444 - HP15_220 0.54 -1.5 208,486 - HP15_220 0.65 -0.5 208,505 - HP15_220 0.69 +2.6 208,564 + HP15_220 0.84 -2.4 208,572 - HP15_220 0.86 -1.1
Or see this region's nucleotide sequence