Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2112 and Shewana3_2113 are separated by 19 nucleotides Shewana3_2113 and Shewana3_2114 are separated by 8 nucleotides Shewana3_2114 and Shewana3_2115 are separated by 55 nucleotides Shewana3_2115 and Shewana3_2116 are separated by 142 nucleotides
Shewana3_2112: Shewana3_2112 - heavy metal translocating P-type ATPase (RefSeq), at 2,535,414 to 2,537,825
_2112
Shewana3_2113: Shewana3_2113 - cytochrome oxidase maturation protein, cbb3-type (RefSeq), at 2,537,845 to 2,538,045
_2113
Shewana3_2114: Shewana3_2114 - hypothetical protein (RefSeq), at 2,538,054 to 2,538,746
_2114
Shewana3_2115: Shewana3_2115 - fumarate/nitrate reduction transcriptional regulator (RefSeq), at 2,538,802 to 2,539,554
_2115
Shewana3_2116: Shewana3_2116 - universal stress protein UspE (RefSeq), at 2,539,697 to 2,540,629
_2116
Position (kb)
2538
2539
2540 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2538.031 kb on + strand at 2538.163 kb on + strand, within Shewana3_2114 at 2538.387 kb on + strand, within Shewana3_2114 at 2538.516 kb on - strand, within Shewana3_2114 at 2538.536 kb on - strand, within Shewana3_2114 at 2538.748 kb on - strand at 2539.154 kb on + strand, within Shewana3_2115 at 2539.554 kb on + strand at 2539.666 kb on + strand at 2540.386 kb on - strand, within Shewana3_2116
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,538,031 + -1.8 2,538,163 + Shewana3_2114 0.16 -2.3 2,538,387 + Shewana3_2114 0.48 -2.8 2,538,516 - Shewana3_2114 0.67 -4.6 2,538,536 - Shewana3_2114 0.70 -1.0 2,538,748 - -0.3 2,539,154 + Shewana3_2115 0.47 +0.1 2,539,554 + +0.1 2,539,666 + +0.2 2,540,386 - Shewana3_2116 0.74 -1.4
Or see this region's nucleotide sequence