Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infC and rpmI are separated by 85 nucleotides rpmI and rplT are separated by 16 nucleotides rplT and Shewana3_2047 are separated by 193 nucleotides Shewana3_2047 and Shewana3_2048 are separated by 250 nucleotides
Shewana3_2044: infC - translation initiation factor IF-3 (RefSeq), at 2,453,312 to 2,453,743
infC
Shewana3_2045: rpmI - 50S ribosomal protein L35 (RefSeq), at 2,453,829 to 2,454,023
rpmI
Shewana3_2046: rplT - 50S ribosomal protein L20 (RefSeq), at 2,454,040 to 2,454,396
rplT
Shewana3_2047: Shewana3_2047 - thioredoxin reductase (RefSeq), at 2,454,590 to 2,455,543
_2047
Shewana3_2048: Shewana3_2048 - L-alanine dehydrogenase (RefSeq), at 2,455,794 to 2,456,909
_2048
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2454.614 kb on - strand at 2455.093 kb on - strand, within Shewana3_2047 at 2455.175 kb on + strand at 2455.175 kb on + strand, within Shewana3_2047 at 2455.183 kb on - strand, within Shewana3_2047 at 2455.183 kb on - strand, within Shewana3_2047 at 2455.219 kb on + strand, within Shewana3_2047 at 2455.457 kb on - strand at 2455.492 kb on - strand at 2455.584 kb on + strand at 2455.673 kb on - strand at 2456.135 kb on - strand, within Shewana3_2048 at 2456.364 kb on + strand, within Shewana3_2048
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,454,614 - +0.8 2,455,093 - Shewana3_2047 0.53 -0.1 2,455,175 + -0.4 2,455,175 + Shewana3_2047 0.61 -1.3 2,455,183 - Shewana3_2047 0.62 +0.2 2,455,183 - Shewana3_2047 0.62 +0.5 2,455,219 + Shewana3_2047 0.66 -1.0 2,455,457 - -0.4 2,455,492 - +2.2 2,455,584 + +0.8 2,455,673 - +2.7 2,456,135 - Shewana3_2048 0.31 -3.9 2,456,364 + Shewana3_2048 0.51 -2.8
Or see this region's nucleotide sequence