Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4149 and Shewana3_4150 are separated by 130 nucleotides Shewana3_4150 and Shewana3_4151 are separated by 375 nucleotides Shewana3_4151 and Shewana3_4152 are separated by 41 nucleotides Shewana3_4152 and Shewana3_4153 are separated by 4 nucleotides Shewana3_4153 and Shewana3_4154 overlap by 4 nucleotides
Shewana3_4149: Shewana3_4149 - IS605 family transposase OrfB (RefSeq), at 10,342 to 11,445
_4149
Shewana3_4150: Shewana3_4150 - hypothetical protein (RefSeq), at 11,576 to 11,842
_4150
Shewana3_4151: Shewana3_4151 - hypothetical protein (RefSeq), at 12,218 to 12,505
_4151
Shewana3_4152: Shewana3_4152 - lipoprotein signal peptidase-like protein (RefSeq), at 12,547 to 12,888
_4152
Shewana3_4153: Shewana3_4153 - nitrogen regulatory protein P-II (RefSeq), at 12,893 to 13,294
_4153
Shewana3_4154: Shewana3_4154 - CzcA family heavy metal efflux protein (RefSeq), at 13,291 to 16,431
_4154
Position (kb) 12
13 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 11.505 kb on + strand at 11.801 kb on + strand, within Shewana3_4150 at 11.806 kb on + strand, within Shewana3_4150 at 12.292 kb on - strand, within Shewana3_4151 at 12.542 kb on - strand at 12.666 kb on - strand, within Shewana3_4152 at 12.717 kb on - strand, within Shewana3_4152 at 12.941 kb on - strand, within Shewana3_4153 at 13.038 kb on - strand, within Shewana3_4153 at 13.066 kb on + strand, within Shewana3_4153 at 13.090 kb on + strand, within Shewana3_4153 at 13.356 kb on + strand at 13.392 kb on + strand at 13.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 11,505 + +3.8 11,801 + Shewana3_4150 0.84 -1.2 11,806 + Shewana3_4150 0.86 +0.2 12,292 - Shewana3_4151 0.26 +1.3 12,542 - +0.8 12,666 - Shewana3_4152 0.35 +0.8 12,717 - Shewana3_4152 0.50 +1.0 12,941 - Shewana3_4153 0.12 -0.2 13,038 - Shewana3_4153 0.36 +0.1 13,066 + Shewana3_4153 0.43 -1.0 13,090 + Shewana3_4153 0.49 -0.4 13,356 + +1.4 13,392 + +0.1 13,482 - -0.1
Or see this region's nucleotide sequence