Experiment: rich media with sodium chlorite 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0121 and Dsui_0122 are separated by 100 nucleotides Dsui_0122 and Dsui_0123 are separated by 3 nucleotides Dsui_0123 and Dsui_0124 are separated by 9 nucleotides Dsui_0124 and Dsui_0125 are separated by 4 nucleotides
Dsui_0121: Dsui_0121 - glutamyl-tRNA reductase, at 119,122 to 120,393
_0121
Dsui_0122: Dsui_0122 - D-tyrosyl-tRNA(Tyr) deacylase, at 120,494 to 120,964
_0122
Dsui_0123: Dsui_0123 - hypothetical protein, at 120,968 to 121,624
_0123
Dsui_0124: Dsui_0124 - lactate dehydrogenase-like oxidoreductase, at 121,634 to 122,602
_0124
Dsui_0125: Dsui_0125 - NAD-dependent aldehyde dehydrogenase, at 122,607 to 123,344
_0125
Position (kb) 120
121
122 Strain fitness (log2 ratio) -1
0
1
2 at 120.429 kb on - strand at 120.447 kb on - strand at 120.517 kb on + strand at 120.714 kb on - strand, within Dsui_0122 at 120.745 kb on + strand at 120.753 kb on - strand, within Dsui_0122 at 120.889 kb on - strand, within Dsui_0122 at 120.907 kb on + strand at 120.974 kb on - strand at 120.974 kb on - strand at 121.029 kb on - strand at 121.036 kb on - strand, within Dsui_0123 at 121.052 kb on + strand, within Dsui_0123 at 121.138 kb on - strand, within Dsui_0123 at 121.149 kb on - strand, within Dsui_0123 at 121.219 kb on + strand, within Dsui_0123 at 121.219 kb on + strand at 121.219 kb on + strand at 121.250 kb on + strand, within Dsui_0123 at 121.265 kb on + strand at 121.399 kb on - strand, within Dsui_0123 at 121.445 kb on - strand, within Dsui_0123 at 121.518 kb on - strand, within Dsui_0123 at 121.683 kb on + strand at 121.683 kb on + strand at 121.691 kb on - strand at 121.710 kb on + strand at 121.736 kb on + strand at 121.737 kb on + strand at 121.783 kb on + strand, within Dsui_0124 at 121.783 kb on + strand at 121.791 kb on - strand, within Dsui_0124 at 121.842 kb on - strand, within Dsui_0124 at 121.935 kb on - strand, within Dsui_0124 at 121.935 kb on - strand, within Dsui_0124 at 121.997 kb on + strand, within Dsui_0124 at 122.005 kb on - strand, within Dsui_0124 at 122.043 kb on + strand at 122.043 kb on - strand, within Dsui_0124 at 122.065 kb on - strand, within Dsui_0124 at 122.105 kb on + strand, within Dsui_0124 at 122.126 kb on + strand at 122.139 kb on - strand, within Dsui_0124 at 122.155 kb on + strand, within Dsui_0124 at 122.155 kb on + strand at 122.156 kb on + strand at 122.217 kb on - strand, within Dsui_0124 at 122.252 kb on + strand, within Dsui_0124 at 122.281 kb on - strand, within Dsui_0124 at 122.314 kb on - strand, within Dsui_0124 at 122.315 kb on - strand, within Dsui_0124 at 122.341 kb on - strand, within Dsui_0124 at 122.359 kb on - strand, within Dsui_0124 at 122.361 kb on + strand, within Dsui_0124 at 122.449 kb on - strand, within Dsui_0124 at 122.519 kb on - strand at 122.526 kb on + strand at 122.534 kb on - strand at 122.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with sodium chlorite 0.16 mM remove 120,429 - -1.6 120,447 - -0.3 120,517 + +0.2 120,714 - Dsui_0122 0.47 -0.9 120,745 + +1.3 120,753 - Dsui_0122 0.55 -1.0 120,889 - Dsui_0122 0.84 -0.5 120,907 + +1.7 120,974 - -0.2 120,974 - -0.4 121,029 - -1.5 121,036 - Dsui_0123 0.10 -0.8 121,052 + Dsui_0123 0.13 -1.0 121,138 - Dsui_0123 0.26 -0.6 121,149 - Dsui_0123 0.28 -0.9 121,219 + Dsui_0123 0.38 +1.0 121,219 + +2.0 121,219 + +2.0 121,250 + Dsui_0123 0.43 +1.7 121,265 + +2.0 121,399 - Dsui_0123 0.66 -1.2 121,445 - Dsui_0123 0.73 +2.0 121,518 - Dsui_0123 0.84 -0.6 121,683 + -0.7 121,683 + +0.4 121,691 - -1.2 121,710 + +0.0 121,736 + +0.4 121,737 + +2.0 121,783 + Dsui_0124 0.15 -0.4 121,783 + +1.0 121,791 - Dsui_0124 0.16 -0.3 121,842 - Dsui_0124 0.21 +0.2 121,935 - Dsui_0124 0.31 +0.4 121,935 - Dsui_0124 0.31 -0.3 121,997 + Dsui_0124 0.37 +0.8 122,005 - Dsui_0124 0.38 +0.8 122,043 + +1.0 122,043 - Dsui_0124 0.42 +0.2 122,065 - Dsui_0124 0.44 -0.2 122,105 + Dsui_0124 0.49 +1.5 122,126 + +0.4 122,139 - Dsui_0124 0.52 +0.4 122,155 + Dsui_0124 0.54 -0.2 122,155 + +2.6 122,156 + +2.0 122,217 - Dsui_0124 0.60 -1.0 122,252 + Dsui_0124 0.64 +0.6 122,281 - Dsui_0124 0.67 -0.1 122,314 - Dsui_0124 0.70 -0.4 122,315 - Dsui_0124 0.70 +0.5 122,341 - Dsui_0124 0.73 -0.5 122,359 - Dsui_0124 0.75 +1.0 122,361 + Dsui_0124 0.75 +1.0 122,449 - Dsui_0124 0.84 +0.0 122,519 - +0.1 122,526 + -0.3 122,534 - +1.0 122,605 + +0.4
Or see this region's nucleotide sequence