Experiment: rich media with sodium chlorite 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0044 and Dsui_0045 are separated by 6 nucleotides Dsui_0045 and Dsui_0046 are separated by 34 nucleotides Dsui_0046 and Dsui_0047 are separated by 111 nucleotides
Dsui_0044: Dsui_0044 - exodeoxyribonuclease III, at 40,231 to 41,004
_0044
Dsui_0045: Dsui_0045 - glutaredoxin-like protein, at 41,011 to 41,520
_0045
Dsui_0046: Dsui_0046 - Zn-dependent oligopeptidase, at 41,555 to 43,636
_0046
Dsui_0047: Dsui_0047 - putative integral membrane protein, at 43,748 to 44,518
_0047
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -1
0
1
2
3 at 40.674 kb on + strand, within Dsui_0044 at 40.783 kb on + strand, within Dsui_0044 at 40.873 kb on - strand, within Dsui_0044 at 41.000 kb on - strand at 41.027 kb on + strand at 41.094 kb on - strand, within Dsui_0045 at 41.215 kb on + strand, within Dsui_0045 at 41.274 kb on + strand at 41.279 kb on + strand at 41.324 kb on + strand, within Dsui_0045 at 41.332 kb on - strand, within Dsui_0045 at 41.403 kb on - strand, within Dsui_0045 at 41.459 kb on + strand, within Dsui_0045 at 41.467 kb on - strand, within Dsui_0045 at 41.485 kb on - strand at 41.506 kb on - strand at 41.658 kb on + strand at 41.793 kb on + strand, within Dsui_0046 at 41.825 kb on + strand, within Dsui_0046 at 41.899 kb on - strand, within Dsui_0046 at 41.947 kb on - strand, within Dsui_0046 at 42.048 kb on + strand at 42.095 kb on - strand, within Dsui_0046 at 42.159 kb on + strand, within Dsui_0046 at 42.173 kb on - strand, within Dsui_0046 at 42.245 kb on - strand, within Dsui_0046 at 42.246 kb on - strand, within Dsui_0046 at 42.383 kb on - strand, within Dsui_0046 at 42.506 kb on + strand, within Dsui_0046 at 42.513 kb on + strand, within Dsui_0046 at 42.646 kb on - strand, within Dsui_0046 at 42.679 kb on - strand, within Dsui_0046 at 42.750 kb on + strand at 42.750 kb on + strand, within Dsui_0046 at 42.760 kb on + strand at 42.813 kb on - strand, within Dsui_0046 at 42.821 kb on + strand, within Dsui_0046 at 42.834 kb on - strand, within Dsui_0046 at 42.836 kb on + strand, within Dsui_0046 at 42.896 kb on + strand at 42.981 kb on + strand, within Dsui_0046 at 43.166 kb on - strand, within Dsui_0046 at 43.272 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.311 kb on - strand, within Dsui_0046 at 43.422 kb on + strand at 43.572 kb on - strand at 43.596 kb on - strand at 43.601 kb on - strand at 43.642 kb on + strand at 43.642 kb on + strand at 43.655 kb on - strand at 43.694 kb on + strand at 43.815 kb on - strand at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.874 kb on - strand, within Dsui_0047 at 44.022 kb on - strand, within Dsui_0047 at 44.184 kb on - strand, within Dsui_0047 at 44.535 kb on + strand at 44.547 kb on + strand at 44.563 kb on + strand at 44.595 kb on + strand at 44.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with sodium chlorite 0.16 mM remove 40,674 + Dsui_0044 0.57 +1.0 40,783 + Dsui_0044 0.71 -0.0 40,873 - Dsui_0044 0.83 -0.9 41,000 - -1.2 41,027 + +0.2 41,094 - Dsui_0045 0.16 -0.4 41,215 + Dsui_0045 0.40 +0.3 41,274 + +1.0 41,279 + +2.0 41,324 + Dsui_0045 0.61 +0.6 41,332 - Dsui_0045 0.63 +0.3 41,403 - Dsui_0045 0.77 +0.8 41,459 + Dsui_0045 0.88 -0.2 41,467 - Dsui_0045 0.89 +0.4 41,485 - -0.0 41,506 - +0.4 41,658 + -0.4 41,793 + Dsui_0046 0.11 -0.9 41,825 + Dsui_0046 0.13 -0.3 41,899 - Dsui_0046 0.17 +0.5 41,947 - Dsui_0046 0.19 +0.1 42,048 + +1.6 42,095 - Dsui_0046 0.26 -0.1 42,159 + Dsui_0046 0.29 +0.4 42,173 - Dsui_0046 0.30 +1.7 42,245 - Dsui_0046 0.33 +0.3 42,246 - Dsui_0046 0.33 +0.1 42,383 - Dsui_0046 0.40 -0.0 42,506 + Dsui_0046 0.46 +0.4 42,513 + Dsui_0046 0.46 -0.1 42,646 - Dsui_0046 0.52 -0.4 42,679 - Dsui_0046 0.54 +0.0 42,750 + +3.6 42,750 + Dsui_0046 0.57 +1.5 42,760 + +0.4 42,813 - Dsui_0046 0.60 -0.4 42,821 + Dsui_0046 0.61 +0.7 42,834 - Dsui_0046 0.61 +0.3 42,836 + Dsui_0046 0.62 -1.2 42,896 + +2.0 42,981 + Dsui_0046 0.68 +0.4 43,166 - Dsui_0046 0.77 -1.2 43,272 - Dsui_0046 0.82 -0.5 43,292 - Dsui_0046 0.83 -0.2 43,292 - Dsui_0046 0.83 +0.2 43,292 - Dsui_0046 0.83 +0.5 43,311 - Dsui_0046 0.84 +0.1 43,422 + +1.0 43,572 - -0.4 43,596 - -1.6 43,601 - +0.1 43,642 + +0.2 43,642 + +2.0 43,655 - -0.1 43,694 + -1.7 43,815 - +0.4 43,851 - Dsui_0047 0.13 -0.5 43,851 - Dsui_0047 0.13 +0.7 43,851 - Dsui_0047 0.13 +0.0 43,874 - Dsui_0047 0.16 +0.5 44,022 - Dsui_0047 0.36 +0.7 44,184 - Dsui_0047 0.57 +0.9 44,535 + +1.0 44,547 + +0.7 44,563 + -0.3 44,595 + -1.2 44,632 - +0.1
Or see this region's nucleotide sequence