Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4393 and pspE-2 are separated by 132 nucleotides pspE-2 and SO4395 are separated by 39 nucleotides SO4395 and acpD are separated by 61 nucleotides acpD and SO4397 are separated by 134 nucleotides
SO4393: SO4393 - acetyltransferase, GNAT family (NCBI ptt file), at 4,589,077 to 4,589,532
SO4393
SO4394: pspE-2 - phage shock protein E (NCBI ptt file), at 4,589,665 to 4,589,991
pspE-2
SO4395: SO4395 - hypothetical protein (NCBI ptt file), at 4,590,031 to 4,590,195
SO4395
SO4396: acpD - acyl carrier protein phosphodiesterase (NCBI ptt file), at 4,590,257 to 4,590,853
acpD
SO4397: SO4397 - hypothetical acetyltransferase (NCBI ptt file), at 4,590,988 to 4,591,425
SO4397
Position (kb) 4590
4591 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4589.180 kb on + strand, within SO4393 at 4589.188 kb on - strand, within SO4393 at 4589.203 kb on - strand, within SO4393 at 4589.235 kb on - strand, within SO4393 at 4589.445 kb on - strand, within SO4393 at 4589.552 kb on - strand at 4589.839 kb on - strand, within pspE-2 at 4589.839 kb on - strand, within pspE-2 at 4589.861 kb on + strand, within pspE-2 at 4589.869 kb on - strand, within pspE-2 at 4589.869 kb on - strand, within pspE-2 at 4589.979 kb on + strand at 4589.979 kb on + strand at 4589.979 kb on + strand at 4589.987 kb on - strand at 4590.022 kb on - strand at 4590.078 kb on + strand, within SO4395 at 4590.078 kb on + strand, within SO4395 at 4590.078 kb on + strand, within SO4395 at 4590.086 kb on - strand, within SO4395 at 4590.086 kb on - strand, within SO4395 at 4590.086 kb on - strand, within SO4395 at 4590.124 kb on + strand, within SO4395 at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.318 kb on + strand, within acpD at 4590.334 kb on - strand, within acpD at 4590.339 kb on + strand, within acpD at 4590.339 kb on + strand, within acpD at 4590.339 kb on + strand, within acpD at 4590.404 kb on - strand, within acpD at 4590.508 kb on - strand, within acpD at 4590.511 kb on + strand, within acpD at 4590.514 kb on - strand, within acpD at 4590.589 kb on + strand, within acpD at 4590.592 kb on - strand, within acpD at 4590.597 kb on - strand, within acpD at 4590.624 kb on + strand, within acpD at 4590.664 kb on + strand, within acpD at 4590.672 kb on - strand, within acpD at 4590.677 kb on + strand, within acpD at 4590.742 kb on - strand, within acpD at 4590.912 kb on + strand at 4590.914 kb on - strand at 4591.016 kb on + strand at 4591.024 kb on - strand at 4591.036 kb on - strand, within SO4397 at 4591.059 kb on - strand, within SO4397 at 4591.115 kb on + strand, within SO4397 at 4591.185 kb on - strand, within SO4397
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 4,589,180 + SO4393 0.23 -0.2 4,589,188 - SO4393 0.24 +0.4 4,589,203 - SO4393 0.28 -1.2 4,589,235 - SO4393 0.35 -1.6 4,589,445 - SO4393 0.81 -0.5 4,589,552 - -0.5 4,589,839 - pspE-2 SO4394 0.53 -0.2 4,589,839 - pspE-2 SO4394 0.53 +1.0 4,589,861 + pspE-2 SO4394 0.60 +1.0 4,589,869 - pspE-2 SO4394 0.62 -0.0 4,589,869 - pspE-2 SO4394 0.62 +0.1 4,589,979 + +0.8 4,589,979 + -0.5 4,589,979 + +0.9 4,589,987 - +0.4 4,590,022 - -0.4 4,590,078 + SO4395 0.28 -0.2 4,590,078 + SO4395 0.28 -3.2 4,590,078 + SO4395 0.28 -1.7 4,590,086 - SO4395 0.33 -0.6 4,590,086 - SO4395 0.33 -1.7 4,590,086 - SO4395 0.33 -0.6 4,590,124 + SO4395 0.56 -0.0 4,590,289 - -0.3 4,590,289 - -0.1 4,590,289 - +1.3 4,590,289 - +0.3 4,590,289 - -1.8 4,590,289 - -0.4 4,590,318 + acpD SO4396 0.10 -0.0 4,590,334 - acpD SO4396 0.13 -0.4 4,590,339 + acpD SO4396 0.14 +0.1 4,590,339 + acpD SO4396 0.14 -1.4 4,590,339 + acpD SO4396 0.14 +1.5 4,590,404 - acpD SO4396 0.25 -0.9 4,590,508 - acpD SO4396 0.42 +0.1 4,590,511 + acpD SO4396 0.43 -0.4 4,590,514 - acpD SO4396 0.43 +0.3 4,590,589 + acpD SO4396 0.56 +0.1 4,590,592 - acpD SO4396 0.56 +0.4 4,590,597 - acpD SO4396 0.57 +0.7 4,590,624 + acpD SO4396 0.61 +0.4 4,590,664 + acpD SO4396 0.68 -0.2 4,590,672 - acpD SO4396 0.70 -0.2 4,590,677 + acpD SO4396 0.70 -0.6 4,590,742 - acpD SO4396 0.81 +0.1 4,590,912 + -0.1 4,590,914 - +0.4 4,591,016 + +1.2 4,591,024 - +0.4 4,591,036 - SO4397 0.11 -0.7 4,591,059 - SO4397 0.16 -0.8 4,591,115 + SO4397 0.29 -0.2 4,591,185 - SO4397 0.45 -1.5
Or see this region's nucleotide sequence