Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemC and hemD are separated by 4 nucleotides hemD and hemX are separated by 60 nucleotides hemX and SO4316 overlap by 4 nucleotides
SO4313: hemC - porphobilinogen deaminase (NCBI ptt file), at 4,490,624 to 4,491,556
hemC
SO4314: hemD - uroporphyrinogen-III synthase (NCBI ptt file), at 4,491,561 to 4,492,301
hemD
SO4315: hemX - hemX protein (NCBI ptt file), at 4,492,362 to 4,493,606
hemX
SO4316: SO4316 - hemY protein, putative (NCBI ptt file), at 4,493,603 to 4,494,769
SO4316
Position (kb)
4492
4493
4494 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4492.288 kb on + strand at 4492.529 kb on + strand, within hemX at 4492.598 kb on + strand, within hemX at 4492.629 kb on + strand, within hemX at 4492.630 kb on + strand, within hemX at 4492.630 kb on + strand, within hemX at 4492.630 kb on + strand, within hemX at 4492.638 kb on - strand, within hemX at 4492.638 kb on - strand, within hemX at 4492.648 kb on + strand, within hemX at 4492.842 kb on - strand, within hemX at 4492.864 kb on - strand, within hemX at 4492.880 kb on + strand, within hemX at 4492.885 kb on + strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.888 kb on - strand, within hemX at 4492.893 kb on - strand, within hemX at 4492.893 kb on - strand, within hemX at 4492.959 kb on + strand, within hemX at 4492.995 kb on + strand, within hemX at 4493.031 kb on + strand, within hemX at 4493.112 kb on - strand, within hemX at 4493.112 kb on - strand, within hemX at 4493.448 kb on - strand, within hemX at 4493.456 kb on - strand, within hemX at 4493.457 kb on + strand, within hemX at 4493.692 kb on - strand at 4493.764 kb on + strand, within SO4316 at 4493.807 kb on + strand, within SO4316 at 4493.839 kb on - strand, within SO4316 at 4493.865 kb on + strand, within SO4316 at 4493.940 kb on + strand, within SO4316 at 4494.107 kb on + strand, within SO4316 at 4494.109 kb on - strand, within SO4316 at 4494.112 kb on + strand, within SO4316 at 4494.164 kb on + strand, within SO4316 at 4494.177 kb on + strand, within SO4316 at 4494.185 kb on - strand, within SO4316 at 4494.185 kb on - strand, within SO4316 at 4494.190 kb on - strand, within SO4316 at 4494.210 kb on - strand, within SO4316 at 4494.292 kb on + strand, within SO4316 at 4494.292 kb on - strand, within SO4316 at 4494.336 kb on + strand, within SO4316 at 4494.344 kb on - strand, within SO4316 at 4494.374 kb on - strand, within SO4316 at 4494.410 kb on + strand, within SO4316 at 4494.418 kb on - strand, within SO4316
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 4,492,288 + -0.8 4,492,529 + hemX SO4315 0.13 -1.6 4,492,598 + hemX SO4315 0.19 -0.9 4,492,629 + hemX SO4315 0.21 -1.9 4,492,630 + hemX SO4315 0.22 -1.0 4,492,630 + hemX SO4315 0.22 +1.9 4,492,630 + hemX SO4315 0.22 -1.9 4,492,638 - hemX SO4315 0.22 -2.7 4,492,638 - hemX SO4315 0.22 -2.0 4,492,648 + hemX SO4315 0.23 -2.0 4,492,842 - hemX SO4315 0.39 -1.4 4,492,864 - hemX SO4315 0.40 -2.3 4,492,880 + hemX SO4315 0.42 -1.7 4,492,885 + hemX SO4315 0.42 -2.0 4,492,888 - hemX SO4315 0.42 -1.4 4,492,888 - hemX SO4315 0.42 -3.1 4,492,888 - hemX SO4315 0.42 +0.3 4,492,888 - hemX SO4315 0.42 -2.7 4,492,893 - hemX SO4315 0.43 -2.9 4,492,893 - hemX SO4315 0.43 -3.7 4,492,959 + hemX SO4315 0.48 -1.4 4,492,995 + hemX SO4315 0.51 -1.9 4,493,031 + hemX SO4315 0.54 -1.6 4,493,112 - hemX SO4315 0.60 -3.8 4,493,112 - hemX SO4315 0.60 -2.3 4,493,448 - hemX SO4315 0.87 -2.9 4,493,456 - hemX SO4315 0.88 -1.6 4,493,457 + hemX SO4315 0.88 -2.4 4,493,692 - +0.0 4,493,764 + SO4316 0.14 -1.1 4,493,807 + SO4316 0.17 -1.0 4,493,839 - SO4316 0.20 -1.1 4,493,865 + SO4316 0.22 -0.1 4,493,940 + SO4316 0.29 -2.5 4,494,107 + SO4316 0.43 -1.1 4,494,109 - SO4316 0.43 +0.2 4,494,112 + SO4316 0.44 -0.8 4,494,164 + SO4316 0.48 -0.6 4,494,177 + SO4316 0.49 -1.6 4,494,185 - SO4316 0.50 -1.4 4,494,185 - SO4316 0.50 +0.5 4,494,190 - SO4316 0.50 -2.9 4,494,210 - SO4316 0.52 -0.7 4,494,292 + SO4316 0.59 +0.3 4,494,292 - SO4316 0.59 -0.6 4,494,336 + SO4316 0.63 -0.1 4,494,344 - SO4316 0.63 -1.1 4,494,374 - SO4316 0.66 -0.4 4,494,410 + SO4316 0.69 -1.4 4,494,418 - SO4316 0.70 +0.0
Or see this region's nucleotide sequence