Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4303 and SO4304 are separated by 5 nucleotides SO4304 and SO4305 are separated by 125 nucleotides SO4305 and xerC overlap by 1 nucleotides xerC and SO4307 are separated by 41 nucleotides
SO4303: SO4303 - hypothetical protein (NCBI ptt file), at 4,481,686 to 4,482,189
SO4303
SO4304: SO4304 - conserved hypothetical protein (NCBI ptt file), at 4,482,195 to 4,482,770
SO4304
SO4305: SO4305 - HAD-superfamily hydrolase, subfamily IA, variant 1 family protein (NCBI ptt file), at 4,482,896 to 4,483,618
SO4305
SO4306: xerC - integrase/recombinase XerC (NCBI ptt file), at 4,483,618 to 4,484,517
xerC
SO4307: SO4307 - conserved hypothetical protein (NCBI ptt file), at 4,484,559 to 4,485,179
SO4307
Position (kb)
4482
4483
4484 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4481.937 kb on - strand, within SO4303 at 4482.035 kb on + strand, within SO4303 at 4482.038 kb on - strand, within SO4303 at 4482.145 kb on - strand at 4482.164 kb on + strand at 4482.172 kb on - strand at 4482.256 kb on + strand, within SO4304 at 4482.259 kb on - strand, within SO4304 at 4482.304 kb on - strand, within SO4304 at 4482.304 kb on - strand, within SO4304 at 4482.389 kb on + strand, within SO4304 at 4482.389 kb on + strand, within SO4304 at 4482.544 kb on - strand, within SO4304 at 4482.623 kb on + strand, within SO4304 at 4482.671 kb on + strand, within SO4304 at 4482.672 kb on + strand, within SO4304 at 4482.679 kb on - strand, within SO4304 at 4482.723 kb on - strand at 4482.838 kb on + strand at 4482.846 kb on - strand at 4482.861 kb on + strand at 4482.947 kb on + strand at 4482.947 kb on + strand at 4482.955 kb on + strand at 4482.955 kb on - strand at 4482.972 kb on + strand, within SO4305 at 4483.044 kb on + strand, within SO4305 at 4483.075 kb on + strand, within SO4305 at 4483.083 kb on - strand, within SO4305 at 4483.136 kb on - strand, within SO4305 at 4483.261 kb on - strand, within SO4305 at 4483.347 kb on - strand, within SO4305 at 4483.350 kb on + strand, within SO4305 at 4483.363 kb on - strand, within SO4305 at 4483.568 kb on - strand at 4483.600 kb on + strand at 4483.657 kb on + strand at 4483.666 kb on - strand at 4483.825 kb on + strand, within xerC at 4483.882 kb on - strand, within xerC at 4483.931 kb on - strand, within xerC at 4483.997 kb on + strand, within xerC at 4484.005 kb on - strand, within xerC at 4484.070 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.195 kb on + strand, within xerC at 4484.319 kb on + strand, within xerC at 4484.469 kb on + strand at 4484.500 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.521 kb on - strand at 4484.547 kb on + strand at 4484.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 4,481,937 - SO4303 0.50 -3.6 4,482,035 + SO4303 0.69 -0.4 4,482,038 - SO4303 0.70 -1.3 4,482,145 - -0.1 4,482,164 + +0.7 4,482,172 - +0.6 4,482,256 + SO4304 0.11 +0.4 4,482,259 - SO4304 0.11 +0.1 4,482,304 - SO4304 0.19 -1.2 4,482,304 - SO4304 0.19 -0.1 4,482,389 + SO4304 0.34 +0.6 4,482,389 + SO4304 0.34 +0.7 4,482,544 - SO4304 0.61 +0.0 4,482,623 + SO4304 0.74 +0.0 4,482,671 + SO4304 0.83 +0.3 4,482,672 + SO4304 0.83 +0.1 4,482,679 - SO4304 0.84 +0.0 4,482,723 - +0.9 4,482,838 + +0.5 4,482,846 - +0.1 4,482,861 + +0.6 4,482,947 + -3.0 4,482,947 + -1.9 4,482,955 + -3.4 4,482,955 - -2.2 4,482,972 + SO4305 0.11 -4.0 4,483,044 + SO4305 0.20 -1.6 4,483,075 + SO4305 0.25 -3.0 4,483,083 - SO4305 0.26 -2.4 4,483,136 - SO4305 0.33 -1.0 4,483,261 - SO4305 0.50 -3.5 4,483,347 - SO4305 0.62 -3.2 4,483,350 + SO4305 0.63 -1.6 4,483,363 - SO4305 0.65 -2.4 4,483,568 - -1.3 4,483,600 + -2.9 4,483,657 + -0.8 4,483,666 - -0.2 4,483,825 + xerC SO4306 0.23 -0.8 4,483,882 - xerC SO4306 0.29 -1.2 4,483,931 - xerC SO4306 0.35 -1.0 4,483,997 + xerC SO4306 0.42 -0.3 4,484,005 - xerC SO4306 0.43 -1.5 4,484,070 + xerC SO4306 0.50 -1.0 4,484,195 + xerC SO4306 0.64 +0.0 4,484,195 + xerC SO4306 0.64 -3.1 4,484,195 + xerC SO4306 0.64 -0.8 4,484,319 + xerC SO4306 0.78 -0.2 4,484,469 + -2.7 4,484,500 - -0.4 4,484,521 - +0.8 4,484,521 - -1.5 4,484,521 - +0.1 4,484,521 - +0.1 4,484,547 + -1.8 4,484,581 - -0.7
Or see this region's nucleotide sequence