Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3518 and glnB-2 are separated by 73 nucleotides glnB-2 and SO3520 are separated by 245 nucleotides
SO3518: SO3518 - ISSod4, transposase (NCBI ptt file), at 3,672,859 to 3,674,061
SO3518
SO3519: glnB-2 - nitrogen regulatory protein P-II 1 (NCBI ptt file), at 3,674,135 to 3,674,473
glnB-2
SO3520: SO3520 - type IV pilus biogenesis protein, putative (NCBI ptt file), at 3,674,719 to 3,675,231
SO3520
Position (kb)
3674
3675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3674.224 kb on - strand, within glnB-2 at 3674.316 kb on + strand, within glnB-2 at 3674.341 kb on + strand, within glnB-2 at 3674.344 kb on - strand, within glnB-2 at 3674.363 kb on + strand, within glnB-2 at 3674.395 kb on - strand, within glnB-2 at 3674.509 kb on + strand at 3674.517 kb on + strand at 3674.517 kb on + strand at 3674.517 kb on + strand at 3674.525 kb on - strand at 3674.531 kb on - strand at 3674.533 kb on + strand at 3674.533 kb on + strand at 3674.541 kb on - strand at 3674.679 kb on - strand at 3674.679 kb on - strand at 3674.679 kb on - strand at 3674.690 kb on + strand at 3674.704 kb on + strand at 3674.707 kb on - strand at 3674.723 kb on + strand at 3674.731 kb on - strand at 3674.731 kb on - strand at 3674.733 kb on - strand at 3674.738 kb on + strand at 3674.738 kb on + strand at 3674.738 kb on + strand at 3674.744 kb on + strand at 3674.746 kb on - strand at 3674.746 kb on - strand at 3674.752 kb on - strand at 3674.752 kb on - strand at 3674.752 kb on - strand at 3674.757 kb on + strand at 3674.757 kb on + strand at 3674.759 kb on + strand at 3674.764 kb on + strand at 3674.765 kb on - strand at 3674.785 kb on - strand, within SO3520 at 3674.813 kb on - strand, within SO3520 at 3674.827 kb on - strand, within SO3520 at 3674.829 kb on + strand, within SO3520 at 3674.829 kb on + strand, within SO3520 at 3674.837 kb on - strand, within SO3520 at 3674.837 kb on - strand, within SO3520 at 3674.896 kb on - strand, within SO3520 at 3674.911 kb on + strand, within SO3520 at 3674.930 kb on - strand, within SO3520 at 3674.935 kb on + strand, within SO3520 at 3674.943 kb on - strand, within SO3520 at 3674.982 kb on + strand, within SO3520 at 3674.990 kb on + strand, within SO3520 at 3675.019 kb on + strand, within SO3520 at 3675.021 kb on + strand, within SO3520 at 3675.029 kb on + strand, within SO3520 at 3675.111 kb on - strand, within SO3520 at 3675.159 kb on + strand, within SO3520 at 3675.161 kb on - strand, within SO3520 at 3675.169 kb on + strand, within SO3520 at 3675.193 kb on + strand at 3675.206 kb on - strand at 3675.206 kb on - strand at 3675.206 kb on - strand at 3675.252 kb on - strand at 3675.263 kb on + strand at 3675.265 kb on + strand at 3675.265 kb on + strand at 3675.271 kb on - strand at 3675.273 kb on + strand at 3675.273 kb on + strand at 3675.273 kb on - strand at 3675.275 kb on + strand at 3675.275 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.283 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.290 kb on + strand at 3675.296 kb on - strand at 3675.298 kb on - strand at 3675.298 kb on - strand at 3675.298 kb on - strand at 3675.300 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.316 kb on + strand at 3675.316 kb on - strand at 3675.316 kb on - strand at 3675.316 kb on - strand at 3675.323 kb on - strand at 3675.330 kb on + strand at 3675.330 kb on + strand at 3675.330 kb on + strand at 3675.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 3,674,224 - glnB-2 SO3519 0.26 -0.1 3,674,316 + glnB-2 SO3519 0.53 +1.8 3,674,341 + glnB-2 SO3519 0.61 -0.1 3,674,344 - glnB-2 SO3519 0.62 +0.1 3,674,363 + glnB-2 SO3519 0.67 +1.0 3,674,395 - glnB-2 SO3519 0.77 -1.0 3,674,509 + +0.2 3,674,517 + +0.6 3,674,517 + +0.7 3,674,517 + +0.4 3,674,525 - -0.1 3,674,531 - +0.0 3,674,533 + +0.6 3,674,533 + +0.1 3,674,541 - -2.1 3,674,679 - -0.7 3,674,679 - -0.3 3,674,679 - +1.0 3,674,690 + -0.1 3,674,704 + +0.9 3,674,707 - -0.3 3,674,723 + +0.3 3,674,731 - +0.6 3,674,731 - -0.8 3,674,733 - -0.5 3,674,738 + -0.8 3,674,738 + +0.6 3,674,738 + +0.3 3,674,744 + +0.3 3,674,746 - -0.2 3,674,746 - -0.0 3,674,752 - -0.4 3,674,752 - +0.3 3,674,752 - +0.0 3,674,757 + -1.9 3,674,757 + +0.6 3,674,759 + +0.4 3,674,764 + -0.5 3,674,765 - -0.8 3,674,785 - SO3520 0.13 -0.3 3,674,813 - SO3520 0.18 -0.8 3,674,827 - SO3520 0.21 +0.1 3,674,829 + SO3520 0.21 -0.6 3,674,829 + SO3520 0.21 -0.2 3,674,837 - SO3520 0.23 -0.1 3,674,837 - SO3520 0.23 -0.0 3,674,896 - SO3520 0.35 -1.0 3,674,911 + SO3520 0.37 -1.1 3,674,930 - SO3520 0.41 +0.0 3,674,935 + SO3520 0.42 -1.2 3,674,943 - SO3520 0.44 -1.7 3,674,982 + SO3520 0.51 +0.3 3,674,990 + SO3520 0.53 +0.0 3,675,019 + SO3520 0.58 +0.5 3,675,021 + SO3520 0.59 -0.6 3,675,029 + SO3520 0.60 +0.4 3,675,111 - SO3520 0.76 +0.4 3,675,159 + SO3520 0.86 +0.6 3,675,161 - SO3520 0.86 -0.3 3,675,169 + SO3520 0.88 -0.3 3,675,193 + +0.2 3,675,206 - +0.4 3,675,206 - +0.1 3,675,206 - +0.0 3,675,252 - +0.2 3,675,263 + +0.1 3,675,265 + +0.0 3,675,265 + +1.0 3,675,271 - +0.0 3,675,273 + -0.0 3,675,273 + -0.8 3,675,273 - +0.4 3,675,275 + +0.8 3,675,275 + -0.8 3,675,280 + +0.0 3,675,280 + -0.2 3,675,280 + +0.1 3,675,280 + +0.6 3,675,280 + -0.0 3,675,283 - -1.3 3,675,288 - +0.3 3,675,288 - +0.0 3,675,288 - +1.0 3,675,288 - +0.1 3,675,288 - +0.4 3,675,288 - -0.6 3,675,290 + +0.4 3,675,296 - -0.2 3,675,298 - +0.8 3,675,298 - +0.5 3,675,298 - +0.7 3,675,300 + +0.1 3,675,308 + +0.2 3,675,308 + -2.3 3,675,308 + -0.7 3,675,308 + +0.3 3,675,308 + +0.3 3,675,308 + +0.5 3,675,308 + -2.9 3,675,308 + -0.6 3,675,308 + -0.4 3,675,316 + +0.7 3,675,316 - +0.2 3,675,316 - +0.2 3,675,316 - -1.5 3,675,323 - -0.5 3,675,330 + -0.1 3,675,330 + +0.1 3,675,330 + +0.6 3,675,420 - -1.0
Or see this region's nucleotide sequence