Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliA and flhG overlap by 8 nucleotides flhG and flhF are separated by 34 nucleotides flhF and flhA are separated by 11 nucleotides
SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868
fliA
SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712
flhG
SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123
flhF
SO3213: flhA - flagellar biosynthesis protein FlhA (NCBI ptt file), at 3,353,135 to 3,355,234
flhA
Position (kb)
3351
3352
3353
3354 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3350.845 kb on + strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3351.311 kb on - strand, within flhG at 3351.457 kb on - strand, within flhG at 3351.511 kb on - strand, within flhG at 3351.571 kb on - strand, within flhG at 3351.591 kb on + strand, within flhG at 3351.638 kb on + strand at 3351.646 kb on - strand at 3351.770 kb on - strand at 3351.770 kb on - strand at 3351.982 kb on - strand, within flhF at 3352.025 kb on - strand, within flhF at 3352.032 kb on + strand, within flhF at 3352.045 kb on - strand, within flhF at 3352.109 kb on + strand, within flhF at 3352.260 kb on + strand, within flhF at 3352.310 kb on - strand, within flhF at 3352.383 kb on + strand, within flhF at 3352.621 kb on - strand, within flhF at 3352.690 kb on + strand, within flhF at 3352.743 kb on - strand, within flhF at 3352.896 kb on + strand, within flhF at 3353.048 kb on + strand at 3353.056 kb on - strand at 3353.143 kb on - strand at 3353.234 kb on + strand at 3353.262 kb on - strand at 3353.506 kb on + strand, within flhA at 3353.689 kb on - strand, within flhA at 3353.788 kb on + strand, within flhA at 3353.795 kb on + strand, within flhA at 3353.906 kb on - strand, within flhA at 3353.986 kb on + strand, within flhA at 3354.057 kb on + strand, within flhA at 3354.057 kb on + strand, within flhA at 3354.065 kb on - strand, within flhA
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 3,350,845 + +1.6 3,350,853 - +1.4 3,350,853 - +1.1 3,350,853 - +0.3 3,351,311 - flhG SO3211 0.53 -4.5 3,351,457 - flhG SO3211 0.70 -4.0 3,351,511 - flhG SO3211 0.76 -3.2 3,351,571 - flhG SO3211 0.83 -5.2 3,351,591 + flhG SO3211 0.86 -0.2 3,351,638 + -2.0 3,351,646 - -5.2 3,351,770 - -0.3 3,351,770 - -1.7 3,351,982 - flhF SO3212 0.17 -3.1 3,352,025 - flhF SO3212 0.20 -0.2 3,352,032 + flhF SO3212 0.21 -3.5 3,352,045 - flhF SO3212 0.22 -2.4 3,352,109 + flhF SO3212 0.26 -3.3 3,352,260 + flhF SO3212 0.37 -1.2 3,352,310 - flhF SO3212 0.41 -0.5 3,352,383 + flhF SO3212 0.46 -3.3 3,352,621 - flhF SO3212 0.63 -2.2 3,352,690 + flhF SO3212 0.68 -2.8 3,352,743 - flhF SO3212 0.72 -1.4 3,352,896 + flhF SO3212 0.83 -3.0 3,353,048 + -1.8 3,353,056 - -2.5 3,353,143 - -3.3 3,353,234 + -5.0 3,353,262 - -0.3 3,353,506 + flhA SO3213 0.18 +1.6 3,353,689 - flhA SO3213 0.26 +0.3 3,353,788 + flhA SO3213 0.31 +1.1 3,353,795 + flhA SO3213 0.31 +1.3 3,353,906 - flhA SO3213 0.37 +1.2 3,353,986 + flhA SO3213 0.41 +1.8 3,354,057 + flhA SO3213 0.44 +1.6 3,354,057 + flhA SO3213 0.44 +0.9 3,354,065 - flhA SO3213 0.44 +0.7
Or see this region's nucleotide sequence