Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcd and udk are separated by 29 nucleotides udk and SO2618 are separated by 11 nucleotides SO2618 and metG are separated by 160 nucleotides
SO2616: dcd - deoxycytidine triphosphate deaminase (NCBI ptt file), at 2,752,176 to 2,752,757
dcd
SO2617: udk - uridine kinase (NCBI ptt file), at 2,752,787 to 2,753,425
udk
SO2618: SO2618 - ATP-binding protein, Mrp/Nbp35 family (NCBI ptt file), at 2,753,437 to 2,754,552
SO2618
SO2619: metG - methionyl-tRNA synthetase (NCBI ptt file), at 2,754,713 to 2,756,743
metG
Position (kb)
2753
2754
2755 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2752.526 kb on - strand, within dcd at 2753.076 kb on - strand, within udk at 2753.143 kb on + strand, within udk at 2753.194 kb on - strand, within udk at 2753.221 kb on - strand, within udk at 2753.436 kb on - strand at 2753.623 kb on - strand, within SO2618 at 2753.623 kb on - strand, within SO2618 at 2753.744 kb on - strand, within SO2618 at 2753.907 kb on - strand, within SO2618 at 2753.984 kb on - strand, within SO2618 at 2754.065 kb on + strand, within SO2618 at 2754.157 kb on + strand, within SO2618 at 2754.448 kb on + strand at 2754.456 kb on - strand at 2754.456 kb on - strand at 2754.462 kb on - strand at 2754.501 kb on - strand at 2754.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 2,752,526 - dcd SO2616 0.60 -3.4 2,753,076 - udk SO2617 0.45 +0.5 2,753,143 + udk SO2617 0.56 -0.4 2,753,194 - udk SO2617 0.64 -0.1 2,753,221 - udk SO2617 0.68 +1.1 2,753,436 - +1.8 2,753,623 - SO2618 0.17 -2.8 2,753,623 - SO2618 0.17 -2.7 2,753,744 - SO2618 0.28 -3.3 2,753,907 - SO2618 0.42 -2.8 2,753,984 - SO2618 0.49 -3.4 2,754,065 + SO2618 0.56 -1.3 2,754,157 + SO2618 0.65 -2.7 2,754,448 + -1.2 2,754,456 - -3.9 2,754,456 - -4.0 2,754,462 - -4.5 2,754,501 - -3.2 2,754,501 - -2.7
Or see this region's nucleotide sequence