Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2469 and SO2470 are separated by 295 nucleotides SO2470 and dapE overlap by 4 nucleotides dapE and SO2472 are separated by 5 nucleotides SO2472 and SO2473 are separated by 69 nucleotides
SO2469: SO2469 - hypothetical TonB-dependent receptor (NCBI ptt file), at 2,589,746 to 2,592,421
SO2469
SO2470: SO2470 - conserved hypothetical protein (NCBI ptt file), at 2,592,717 to 2,593,061
SO2470
SO2471: dapE - succinyl-diaminopimelate desuccinylase (NCBI ptt file), at 2,593,058 to 2,594,212
dapE
SO2472: SO2472 - D-alanyl-D-alanine carboxypeptidase-related protein (NCBI ptt file), at 2,594,218 to 2,594,976
SO2472
SO2473: SO2473 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,595,046 to 2,595,879
SO2473
Position (kb)
2593
2594
2595 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2592.061 kb on - strand, within SO2469 at 2592.068 kb on - strand, within SO2469 at 2592.078 kb on + strand, within SO2469 at 2592.157 kb on - strand at 2592.162 kb on - strand at 2592.173 kb on + strand at 2592.181 kb on - strand at 2592.217 kb on - strand at 2592.233 kb on + strand at 2592.241 kb on + strand at 2592.260 kb on + strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.268 kb on - strand at 2592.279 kb on + strand at 2592.318 kb on - strand at 2592.399 kb on - strand at 2592.551 kb on + strand at 2592.615 kb on + strand at 2592.825 kb on + strand, within SO2470 at 2592.938 kb on + strand, within SO2470 at 2594.244 kb on + strand at 2594.257 kb on + strand at 2594.257 kb on + strand at 2594.342 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.801 kb on + strand, within SO2472 at 2594.970 kb on + strand at 2595.096 kb on + strand at 2595.146 kb on + strand, within SO2473 at 2595.148 kb on + strand, within SO2473
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 2,592,061 - SO2469 0.87 +1.1 2,592,068 - SO2469 0.87 -0.9 2,592,078 + SO2469 0.87 +0.2 2,592,157 - -0.5 2,592,162 - -0.3 2,592,173 + -0.6 2,592,181 - +0.3 2,592,217 - +0.9 2,592,233 + +0.2 2,592,241 + -0.8 2,592,260 + -0.1 2,592,268 - +0.6 2,592,268 - -0.0 2,592,268 - +0.4 2,592,268 - +0.7 2,592,268 - -1.3 2,592,279 + +0.6 2,592,318 - -0.1 2,592,399 - +0.2 2,592,551 + +0.3 2,592,615 + +3.5 2,592,825 + SO2470 0.31 -1.0 2,592,938 + SO2470 0.64 -0.7 2,594,244 + +0.6 2,594,257 + -3.2 2,594,257 + -5.6 2,594,342 + SO2472 0.16 -3.4 2,594,801 + SO2472 0.77 -5.7 2,594,801 + SO2472 0.77 -3.9 2,594,970 + -0.9 2,595,096 + +0.2 2,595,146 + SO2473 0.12 -0.0 2,595,148 + SO2473 0.12 -0.4
Or see this region's nucleotide sequence