Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2187 and SO_2188 are separated by 39 nucleotides SO_2188 and SO2189 are separated by 319 nucleotides SO2189 and creA are separated by 17 nucleotides creA and metC are separated by 107 nucleotides
SO2187: SO2187 - ISSod11, transposase (NCBI ptt file), at 2,298,229 to 2,299,185
SO2187
SO_2188: SO_2188 - no description, at 2,299,225 to 2,299,716
_2188
SO2189: SO2189 - hypothetical heat shock protein (NCBI ptt file), at 2,300,036 to 2,301,490
SO2189
SO2190: creA - creA protein (NCBI ptt file), at 2,301,508 to 2,302,005
creA
SO2191: metC - cystathionine beta-lyase (NCBI ptt file), at 2,302,113 to 2,303,312
metC
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2299.716 kb on + strand at 2299.726 kb on + strand at 2299.758 kb on - strand at 2299.855 kb on + strand at 2299.984 kb on + strand at 2299.984 kb on + strand at 2299.992 kb on - strand at 2300.012 kb on + strand at 2300.082 kb on - strand at 2300.145 kb on - strand at 2300.159 kb on - strand at 2300.339 kb on - strand, within SO2189 at 2300.393 kb on + strand, within SO2189 at 2300.401 kb on - strand, within SO2189 at 2300.419 kb on + strand, within SO2189 at 2300.459 kb on - strand, within SO2189 at 2300.548 kb on - strand, within SO2189 at 2300.594 kb on + strand, within SO2189 at 2300.653 kb on + strand, within SO2189 at 2300.796 kb on + strand, within SO2189 at 2300.796 kb on + strand, within SO2189 at 2300.804 kb on - strand, within SO2189 at 2300.804 kb on - strand, within SO2189 at 2300.804 kb on - strand, within SO2189 at 2300.831 kb on + strand, within SO2189 at 2300.909 kb on + strand, within SO2189 at 2301.064 kb on + strand, within SO2189 at 2301.224 kb on + strand, within SO2189 at 2301.592 kb on + strand, within creA at 2302.069 kb on + strand at 2302.081 kb on - strand at 2302.103 kb on + strand at 2302.229 kb on + strand at 2302.386 kb on - strand, within metC at 2302.463 kb on + strand, within metC
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 2,299,716 + -1.6 2,299,726 + -0.2 2,299,758 - -1.0 2,299,855 + -0.9 2,299,984 + +0.2 2,299,984 + +2.2 2,299,992 - -1.5 2,300,012 + -0.4 2,300,082 - +0.2 2,300,145 - -0.0 2,300,159 - -0.3 2,300,339 - SO2189 0.21 -0.5 2,300,393 + SO2189 0.25 -0.8 2,300,401 - SO2189 0.25 -0.4 2,300,419 + SO2189 0.26 -0.3 2,300,459 - SO2189 0.29 -0.1 2,300,548 - SO2189 0.35 -2.7 2,300,594 + SO2189 0.38 +0.8 2,300,653 + SO2189 0.42 -2.1 2,300,796 + SO2189 0.52 -3.0 2,300,796 + SO2189 0.52 +0.6 2,300,804 - SO2189 0.53 -0.8 2,300,804 - SO2189 0.53 -0.8 2,300,804 - SO2189 0.53 -1.0 2,300,831 + SO2189 0.55 +0.5 2,300,909 + SO2189 0.60 -0.7 2,301,064 + SO2189 0.71 -0.5 2,301,224 + SO2189 0.82 +0.1 2,301,592 + creA SO2190 0.17 +0.1 2,302,069 + -0.3 2,302,081 - -2.5 2,302,103 + +1.0 2,302,229 + -0.6 2,302,386 - metC SO2191 0.23 +1.7 2,302,463 + metC SO2191 0.29 +0.4
Or see this region's nucleotide sequence