Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mvaB and SO1894 are separated by 1 nucleotides SO1894 and SO1895 overlap by 1 nucleotides SO1895 and pccB-1 are separated by 46 nucleotides
SO1893: mvaB - hydroxymethylglutaryl-CoA lyase (NCBI ptt file), at 1,989,479 to 1,990,426
mvaB
SO1894: SO1894 - acetyl-CoA carboxylase, biotin carboxylase, putative (NCBI ptt file), at 1,990,428 to 1,992,512
SO1894
SO1895: SO1895 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,992,512 to 1,993,378
SO1895
SO1896: pccB-1 - 3-methylcrotonyl CoA carboxylase, beta subunit (NCBI ptt file), at 1,993,425 to 1,995,032
pccB-1
Position (kb)
1990
1991
1992
1993 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1990.469 kb on + strand at 1990.553 kb on - strand at 1990.591 kb on + strand at 1990.591 kb on + strand at 1990.591 kb on + strand at 1990.599 kb on - strand at 1990.599 kb on - strand at 1990.599 kb on - strand at 1990.623 kb on + strand at 1990.631 kb on - strand at 1990.633 kb on + strand at 1990.700 kb on - strand, within SO1894 at 1990.726 kb on + strand, within SO1894 at 1990.726 kb on + strand, within SO1894 at 1990.734 kb on - strand, within SO1894 at 1990.817 kb on + strand, within SO1894 at 1990.959 kb on - strand, within SO1894 at 1990.964 kb on - strand, within SO1894 at 1990.994 kb on - strand, within SO1894 at 1991.175 kb on - strand, within SO1894 at 1991.262 kb on + strand, within SO1894 at 1991.386 kb on + strand, within SO1894 at 1991.474 kb on + strand, within SO1894 at 1991.482 kb on - strand, within SO1894 at 1991.592 kb on + strand, within SO1894 at 1991.611 kb on + strand, within SO1894 at 1991.789 kb on + strand, within SO1894 at 1992.007 kb on + strand, within SO1894 at 1992.007 kb on + strand, within SO1894 at 1992.015 kb on - strand, within SO1894 at 1992.194 kb on + strand, within SO1894 at 1992.234 kb on + strand, within SO1894 at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.409 kb on + strand at 1992.417 kb on - strand at 1992.417 kb on - strand at 1992.417 kb on - strand at 1992.427 kb on - strand at 1992.495 kb on + strand at 1992.584 kb on + strand at 1992.663 kb on + strand, within SO1895 at 1992.826 kb on + strand, within SO1895 at 1992.968 kb on + strand, within SO1895 at 1992.980 kb on + strand, within SO1895 at 1992.980 kb on + strand, within SO1895 at 1993.056 kb on + strand, within SO1895 at 1993.228 kb on + strand, within SO1895 at 1993.261 kb on + strand, within SO1895 at 1993.312 kb on + strand at 1993.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 1,990,469 + -2.3 1,990,553 - -3.6 1,990,591 + -1.6 1,990,591 + -1.5 1,990,591 + -2.3 1,990,599 - -4.2 1,990,599 - -4.3 1,990,599 - -1.8 1,990,623 + +0.0 1,990,631 - -2.4 1,990,633 + -3.3 1,990,700 - SO1894 0.13 -3.3 1,990,726 + SO1894 0.14 -2.8 1,990,726 + SO1894 0.14 -1.1 1,990,734 - SO1894 0.15 -0.3 1,990,817 + SO1894 0.19 -2.3 1,990,959 - SO1894 0.25 -1.7 1,990,964 - SO1894 0.26 -1.8 1,990,994 - SO1894 0.27 -1.7 1,991,175 - SO1894 0.36 -4.2 1,991,262 + SO1894 0.40 -1.0 1,991,386 + SO1894 0.46 -3.3 1,991,474 + SO1894 0.50 -2.8 1,991,482 - SO1894 0.51 -3.0 1,991,592 + SO1894 0.56 -2.5 1,991,611 + SO1894 0.57 -2.6 1,991,789 + SO1894 0.65 +0.1 1,992,007 + SO1894 0.76 -2.1 1,992,007 + SO1894 0.76 -1.7 1,992,015 - SO1894 0.76 -2.5 1,992,194 + SO1894 0.85 -2.8 1,992,234 + SO1894 0.87 -0.6 1,992,409 + -4.0 1,992,409 + -1.1 1,992,409 + -1.9 1,992,409 + -2.0 1,992,409 + -3.3 1,992,417 - -1.3 1,992,417 - -1.8 1,992,417 - -2.3 1,992,427 - -1.8 1,992,495 + -2.6 1,992,584 + -1.7 1,992,663 + SO1895 0.17 -0.3 1,992,826 + SO1895 0.36 -2.2 1,992,968 + SO1895 0.53 -2.3 1,992,980 + SO1895 0.54 -3.1 1,992,980 + SO1895 0.54 -2.4 1,993,056 + SO1895 0.63 -4.3 1,993,228 + SO1895 0.83 -1.7 1,993,261 + SO1895 0.86 -3.1 1,993,312 + -2.3 1,993,355 + -1.6
Or see this region's nucleotide sequence