Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0701 and SO0702 are separated by 3 nucleotides SO0702 and groES are separated by 227 nucleotides groES and groEL are separated by 58 nucleotides
SO0701: SO0701 - transcriptional regulator, LysR family (NCBI ptt file), at 718,091 to 719,080
SO0701
SO0702: SO0702 - MATE efflux family protein (NCBI ptt file), at 719,084 to 720,442
SO0702
SO0703: groES - chaperonin GroES (NCBI ptt file), at 720,670 to 720,960
groES
SO0704: groEL - chaperonin GroEL (NCBI ptt file), at 721,019 to 722,656
groEL
Position (kb)
719
720
721 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 718.216 kb on + strand, within SO0701 at 718.310 kb on - strand, within SO0701 at 718.357 kb on - strand, within SO0701 at 718.403 kb on - strand, within SO0701 at 718.403 kb on - strand, within SO0701 at 718.433 kb on + strand, within SO0701 at 718.441 kb on - strand, within SO0701 at 718.452 kb on + strand, within SO0701 at 718.470 kb on - strand, within SO0701 at 718.696 kb on + strand, within SO0701 at 718.823 kb on + strand, within SO0701 at 719.008 kb on - strand at 719.014 kb on + strand at 719.031 kb on + strand at 719.032 kb on + strand at 719.177 kb on - strand at 719.177 kb on - strand at 719.240 kb on + strand, within SO0702 at 719.260 kb on + strand, within SO0702 at 719.276 kb on + strand, within SO0702 at 719.386 kb on - strand, within SO0702 at 719.410 kb on - strand, within SO0702 at 719.853 kb on + strand, within SO0702 at 719.934 kb on + strand, within SO0702 at 720.000 kb on - strand, within SO0702 at 720.050 kb on - strand, within SO0702 at 720.136 kb on + strand, within SO0702 at 720.208 kb on + strand, within SO0702 at 720.216 kb on - strand, within SO0702 at 720.307 kb on - strand at 720.348 kb on - strand at 720.382 kb on + strand at 720.387 kb on + strand at 720.390 kb on - strand at 720.392 kb on + strand at 720.395 kb on - strand at 720.397 kb on + strand at 720.419 kb on + strand at 720.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 718,216 + SO0701 0.13 +0.6 718,310 - SO0701 0.22 -1.0 718,357 - SO0701 0.27 +0.9 718,403 - SO0701 0.32 -0.2 718,403 - SO0701 0.32 +0.6 718,433 + SO0701 0.35 -0.6 718,441 - SO0701 0.35 +0.4 718,452 + SO0701 0.36 +0.7 718,470 - SO0701 0.38 +0.8 718,696 + SO0701 0.61 +0.1 718,823 + SO0701 0.74 -0.2 719,008 - +1.0 719,014 + +0.4 719,031 + -1.1 719,032 + -0.3 719,177 - -3.5 719,177 - -4.1 719,240 + SO0702 0.11 -2.1 719,260 + SO0702 0.13 -4.0 719,276 + SO0702 0.14 -4.8 719,386 - SO0702 0.22 -5.4 719,410 - SO0702 0.24 -4.4 719,853 + SO0702 0.57 -0.9 719,934 + SO0702 0.63 -1.3 720,000 - SO0702 0.67 -3.6 720,050 - SO0702 0.71 -2.4 720,136 + SO0702 0.77 -3.9 720,208 + SO0702 0.83 -4.5 720,216 - SO0702 0.83 -4.4 720,307 - -4.1 720,348 - -4.9 720,382 + -2.8 720,387 + -4.0 720,390 - -4.5 720,392 + -3.0 720,395 - -5.0 720,397 + -3.5 720,419 + -3.9 720,432 - -5.9
Or see this region's nucleotide sequence