Experiment: Paraquat dichloride 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutL and miaA overlap by 8 nucleotides miaA and hfq are separated by 91 nucleotides hfq and hflX are separated by 23 nucleotides
SO0601: mutL - DNA mismatch repair protein MutL (NCBI ptt file), at 626,850 to 628,745
mutL
SO0602: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 628,738 to 629,664
miaA
SO0603: hfq - host factor-I protein (NCBI ptt file), at 629,756 to 630,028
hfq
SO0604: hflX - GTP-binding protein HflX (NCBI ptt file), at 630,052 to 631,359
hflX
Position (kb) 628
629
630 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 627.912 kb on - strand, within mutL at 627.972 kb on + strand, within mutL at 627.972 kb on + strand, within mutL at 627.975 kb on + strand, within mutL at 627.978 kb on - strand, within mutL at 628.050 kb on + strand, within mutL at 628.070 kb on - strand, within mutL at 628.126 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.212 kb on - strand, within mutL at 628.529 kb on + strand, within mutL at 628.537 kb on - strand, within mutL at 628.537 kb on - strand, within mutL at 628.695 kb on + strand at 628.891 kb on + strand, within miaA at 629.010 kb on + strand, within miaA at 629.302 kb on + strand, within miaA at 629.395 kb on + strand, within miaA at 629.471 kb on + strand, within miaA at 629.942 kb on + strand, within hfq at 629.999 kb on + strand, within hfq at 630.310 kb on + strand, within hflX at 630.354 kb on + strand, within hflX at 630.359 kb on + strand, within hflX at 630.402 kb on - strand, within hflX at 630.502 kb on + strand, within hflX
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.5 mg/ml remove 627,912 - mutL SO0601 0.56 -0.7 627,972 + mutL SO0601 0.59 -0.2 627,972 + mutL SO0601 0.59 +0.4 627,975 + mutL SO0601 0.59 +0.4 627,978 - mutL SO0601 0.59 -0.6 628,050 + mutL SO0601 0.63 -0.2 628,070 - mutL SO0601 0.64 -2.6 628,126 + mutL SO0601 0.67 -0.3 628,199 + mutL SO0601 0.71 +0.4 628,199 + mutL SO0601 0.71 -1.2 628,199 + mutL SO0601 0.71 +0.9 628,199 + mutL SO0601 0.71 -0.2 628,199 + mutL SO0601 0.71 +0.3 628,199 + mutL SO0601 0.71 -0.0 628,199 + mutL SO0601 0.71 -0.5 628,199 + mutL SO0601 0.71 -1.7 628,207 - mutL SO0601 0.72 -1.1 628,207 - mutL SO0601 0.72 -1.8 628,207 - mutL SO0601 0.72 -2.6 628,207 - mutL SO0601 0.72 -0.7 628,207 - mutL SO0601 0.72 -0.5 628,207 - mutL SO0601 0.72 -0.1 628,207 - mutL SO0601 0.72 -1.9 628,207 - mutL SO0601 0.72 -0.9 628,207 - mutL SO0601 0.72 -3.5 628,207 - mutL SO0601 0.72 -0.2 628,207 - mutL SO0601 0.72 -4.6 628,207 - mutL SO0601 0.72 -0.5 628,207 - mutL SO0601 0.72 -0.7 628,212 - mutL SO0601 0.72 -0.6 628,529 + mutL SO0601 0.89 -4.0 628,537 - mutL SO0601 0.89 -3.0 628,537 - mutL SO0601 0.89 -1.9 628,695 + -4.6 628,891 + miaA SO0602 0.17 -3.2 629,010 + miaA SO0602 0.29 -2.5 629,302 + miaA SO0602 0.61 -3.2 629,395 + miaA SO0602 0.71 -0.5 629,471 + miaA SO0602 0.79 -2.2 629,942 + hfq SO0603 0.68 -5.2 629,999 + hfq SO0603 0.89 -3.1 630,310 + hflX SO0604 0.20 -3.2 630,354 + hflX SO0604 0.23 -5.1 630,359 + hflX SO0604 0.23 -4.4 630,402 - hflX SO0604 0.27 -3.1 630,502 + hflX SO0604 0.34 -4.0
Or see this region's nucleotide sequence