Experiment: rich media with sodium chlorite 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3190 and Dsui_3191 are separated by 169 nucleotides Dsui_3191 and Dsui_3192 are separated by 11 nucleotides Dsui_3192 and Dsui_3193 are separated by 143 nucleotides Dsui_3193 and Dsui_3194 overlap by 4 nucleotides
Dsui_3190: Dsui_3190 - acetyl-CoA carboxylase, carboxyl transferase, beta subunit, at 3,424,390 to 3,425,256
_3190
Dsui_3191: Dsui_3191 - tryptophan synthase, alpha subunit, at 3,425,426 to 3,426,229
_3191
Dsui_3192: Dsui_3192 - tryptophan synthase, beta subunit, at 3,426,241 to 3,427,437
_3192
Dsui_3193: Dsui_3193 - phosphoribosylanthranilate isomerase, at 3,427,581 to 3,428,228
_3193
Dsui_3194: Dsui_3194 - pseudouridylate synthase I, at 3,428,225 to 3,429,052
_3194
Position (kb) 3426
3427
3428 Strain fitness (log2 ratio) -1
0
1 at 3425.352 kb on - strand at 3425.392 kb on - strand at 3425.392 kb on - strand at 3428.262 kb on - strand at 3428.313 kb on - strand, within Dsui_3194 at 3428.394 kb on + strand, within Dsui_3194 at 3428.402 kb on - strand, within Dsui_3194 at 3428.427 kb on - strand, within Dsui_3194
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with sodium chlorite 0.16 mM remove 3,425,352 - -0.5 3,425,392 - +0.2 3,425,392 - +0.1 3,428,262 - +0.9 3,428,313 - Dsui_3194 0.11 -0.6 3,428,394 + Dsui_3194 0.20 -0.4 3,428,402 - Dsui_3194 0.21 +0.2 3,428,427 - Dsui_3194 0.24 -1.2
Or see this region's nucleotide sequence