Experiment: rich media with sodium chlorite 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1312 and Dsui_1313 overlap by 4 nucleotides Dsui_1313 and Dsui_1314 are separated by 3 nucleotides Dsui_1314 and Dsui_1315 are separated by 15 nucleotides Dsui_1315 and Dsui_1316 are separated by 30 nucleotides Dsui_1316 and Dsui_1317 overlap by 22 nucleotides
Dsui_1312: Dsui_1312 - NTP pyrophosphohydrolase, at 1,372,252 to 1,372,719
_1312
Dsui_1313: Dsui_1313 - Barstar, RNAse (barnase) inhibitor, at 1,372,716 to 1,373,162
_1313
Dsui_1314: Dsui_1314 - guanyl-specific ribonuclease Sa, at 1,373,166 to 1,373,558
_1314
Dsui_1315: Dsui_1315 - aspartyl-tRNA synthetase, at 1,373,574 to 1,375,373
_1315
Dsui_1316: Dsui_1316 - hypothetical protein, at 1,375,404 to 1,376,045
_1316
Dsui_1317: Dsui_1317 - putative regulatory protein, FmdB family, at 1,376,024 to 1,376,296
_1317
Position (kb)
1373
1374
1375
1376 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1372.647 kb on + strand, within Dsui_1312 at 1372.649 kb on + strand, within Dsui_1312 at 1372.649 kb on - strand, within Dsui_1312 at 1372.789 kb on - strand, within Dsui_1313 at 1372.824 kb on + strand at 1372.837 kb on - strand, within Dsui_1313 at 1372.837 kb on - strand, within Dsui_1313 at 1372.856 kb on - strand, within Dsui_1313 at 1372.879 kb on + strand at 1372.896 kb on + strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.904 kb on - strand, within Dsui_1313 at 1372.909 kb on - strand, within Dsui_1313 at 1373.110 kb on + strand, within Dsui_1313 at 1373.110 kb on + strand at 1373.118 kb on - strand at 1373.298 kb on + strand at 1373.305 kb on + strand, within Dsui_1314 at 1373.320 kb on + strand at 1373.375 kb on - strand, within Dsui_1314 at 1373.393 kb on + strand, within Dsui_1314 at 1373.394 kb on + strand at 1373.571 kb on + strand at 1373.579 kb on - strand at 1375.453 kb on - strand at 1375.480 kb on - strand, within Dsui_1316 at 1375.481 kb on - strand, within Dsui_1316 at 1375.678 kb on - strand, within Dsui_1316 at 1375.720 kb on - strand, within Dsui_1316 at 1375.956 kb on - strand, within Dsui_1316 at 1376.156 kb on - strand, within Dsui_1317 at 1376.339 kb on + strand at 1376.339 kb on + strand at 1376.339 kb on + strand at 1376.339 kb on + strand at 1376.339 kb on + strand at 1376.339 kb on + strand at 1376.347 kb on - strand at 1376.347 kb on - strand at 1376.347 kb on - strand at 1376.347 kb on - strand at 1376.347 kb on - strand at 1376.349 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with sodium chlorite 0.16 mM remove 1,372,647 + Dsui_1312 0.84 -0.8 1,372,649 + Dsui_1312 0.85 -0.9 1,372,649 - Dsui_1312 0.85 +0.3 1,372,789 - Dsui_1313 0.16 +1.3 1,372,824 + +1.0 1,372,837 - Dsui_1313 0.27 -0.3 1,372,837 - Dsui_1313 0.27 -0.5 1,372,856 - Dsui_1313 0.31 +1.0 1,372,879 + +2.0 1,372,896 + Dsui_1313 0.40 -0.6 1,372,904 - Dsui_1313 0.42 -1.1 1,372,904 - Dsui_1313 0.42 +0.4 1,372,904 - Dsui_1313 0.42 +0.5 1,372,904 - Dsui_1313 0.42 +0.4 1,372,909 - Dsui_1313 0.43 +1.0 1,373,110 + Dsui_1313 0.88 +0.7 1,373,110 + +0.4 1,373,118 - +0.4 1,373,298 + +3.6 1,373,305 + Dsui_1314 0.35 +0.3 1,373,320 + +1.4 1,373,375 - Dsui_1314 0.53 +0.9 1,373,393 + Dsui_1314 0.58 +0.7 1,373,394 + +1.3 1,373,571 + -0.2 1,373,579 - -0.1 1,375,453 - +1.6 1,375,480 - Dsui_1316 0.12 -1.5 1,375,481 - Dsui_1316 0.12 +0.1 1,375,678 - Dsui_1316 0.43 -0.7 1,375,720 - Dsui_1316 0.49 -1.6 1,375,956 - Dsui_1316 0.86 -1.2 1,376,156 - Dsui_1317 0.48 -0.7 1,376,339 + -1.5 1,376,339 + -0.3 1,376,339 + -0.3 1,376,339 + -0.0 1,376,339 + -1.5 1,376,339 + +0.1 1,376,347 - +0.0 1,376,347 - -0.0 1,376,347 - -0.1 1,376,347 - -0.1 1,376,347 - +0.3 1,376,349 + -0.0
Or see this region's nucleotide sequence