Experiment: L-Arginine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3020 and Shewana3_3021 are separated by 65 nucleotides Shewana3_3021 and acpS are separated by 170 nucleotides acpS and Shewana3_3023 overlap by 1 nucleotides Shewana3_3023 and Shewana3_3024 are separated by 89 nucleotides
Shewana3_3020: Shewana3_3020 - hypothetical protein (RefSeq), at 3,626,407 to 3,627,678
_3020
Shewana3_3021: Shewana3_3021 - hypothetical protein (RefSeq), at 3,627,744 to 3,628,280
_3021
Shewana3_3022: acpS - 4'-phosphopantetheinyl transferase (RefSeq), at 3,628,451 to 3,628,834
acpS
Shewana3_3023: Shewana3_3023 - pyridoxine 5'-phosphate synthase (RefSeq), at 3,628,834 to 3,629,571
_3023
Shewana3_3024: Shewana3_3024 - DNA repair protein RecO (RefSeq), at 3,629,661 to 3,630,362
_3024
Position (kb)
3628
3629 Strain fitness (log2 ratio)
-1
0
1
2 at 3627.540 kb on - strand, within Shewana3_3020 at 3627.813 kb on - strand, within Shewana3_3021 at 3628.073 kb on - strand, within Shewana3_3021 at 3628.544 kb on - strand, within acpS at 3628.589 kb on - strand, within acpS at 3628.621 kb on - strand, within acpS at 3628.646 kb on + strand, within acpS at 3628.778 kb on - strand, within acpS at 3629.629 kb on - strand at 3629.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arginine (N) remove 3,627,540 - Shewana3_3020 0.89 -1.2 3,627,813 - Shewana3_3021 0.13 +0.0 3,628,073 - Shewana3_3021 0.61 -0.7 3,628,544 - acpS Shewana3_3022 0.24 +2.1 3,628,589 - acpS Shewana3_3022 0.36 +1.4 3,628,621 - acpS Shewana3_3022 0.44 +0.5 3,628,646 + acpS Shewana3_3022 0.51 +2.4 3,628,778 - acpS Shewana3_3022 0.85 +1.6 3,629,629 - -1.7 3,629,629 - -0.5
Or see this region's nucleotide sequence