Experiment: D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_R0007 and Sama_R0008 are separated by 65 nucleotides Sama_R0008 and Sama_R0009 are separated by 66 nucleotides Sama_R0009 and murB are separated by 181 nucleotides
Sama_R0007: Sama_R0007 - 23S ribosomal RNA (RefSeq), at 226,509 to 229,422
_R0007
Sama_R0008: Sama_R0008 - tRNA-Val (RefSeq), at 229,488 to 229,563
_R0008
Sama_R0009: Sama_R0009 - 5S ribosomal RNA (RefSeq), at 229,630 to 229,745
_R0009
Sama_0196: murB - UDP-N-acetylenolpyruvoylglucosamine reductase (RefSeq), at 229,927 to 230,940
murB
Position (kb) 229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 229.619 kb on + strand at 229.730 kb on + strand, within Sama_R0009 at 229.730 kb on + strand, within Sama_R0009 at 229.749 kb on + strand at 229.749 kb on + strand at 229.749 kb on + strand at 229.749 kb on + strand at 229.749 kb on + strand at 229.749 kb on + strand at 229.749 kb on + strand at 229.752 kb on + strand at 229.752 kb on + strand at 229.818 kb on + strand at 229.818 kb on + strand at 229.821 kb on + strand at 229.821 kb on + strand at 229.822 kb on - strand at 229.894 kb on + strand at 229.894 kb on + strand at 229.894 kb on + strand at 229.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate remove 229,619 + +0.2 229,730 + Sama_R0009 0.86 -0.9 229,730 + Sama_R0009 0.86 -0.3 229,749 + +0.5 229,749 + -0.3 229,749 + -0.4 229,749 + +0.3 229,749 + -0.1 229,749 + -1.1 229,749 + +0.3 229,752 + -2.8 229,752 + -0.9 229,818 + +0.1 229,818 + -0.3 229,821 + -0.1 229,821 + +0.3 229,822 - +0.4 229,894 + -0.0 229,894 + -1.9 229,894 + +0.7 229,907 + +0.1
Or see this region's nucleotide sequence