Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3771 and Shew_3772 are separated by 193 nucleotides Shew_3772 and Shew_3773 are separated by 17 nucleotides Shew_3773 and Shew_3774 are separated by 19 nucleotides Shew_3774 and Shew_3775 are separated by 5 nucleotides Shew_3775 and Shew_3776 are separated by 159 nucleotides
Shew_3771: Shew_3771 - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (RefSeq), at 4,491,388 to 4,492,065
_3771
Shew_3772: Shew_3772 - thioesterase superfamily protein (RefSeq), at 4,492,259 to 4,492,687
_3772
Shew_3773: Shew_3773 - hypothetical protein (RefSeq), at 4,492,705 to 4,493,745
_3773
Shew_3774: Shew_3774 - acetyltransferase-like (RefSeq), at 4,493,765 to 4,494,091
_3774
Shew_3775: Shew_3775 - thioesterase superfamily protein (RefSeq), at 4,494,097 to 4,494,501
_3775
Shew_3776: Shew_3776 - AMP-dependent synthetase and ligase (RefSeq), at 4,494,661 to 4,496,457
_3776
Position (kb)
4492
4493
4494 Strain fitness (log2 ratio)
-2
-1
0
1 at 4491.759 kb on - strand, within Shew_3771 at 4492.059 kb on - strand at 4492.155 kb on - strand at 4492.632 kb on + strand, within Shew_3772 at 4492.787 kb on - strand at 4492.818 kb on + strand, within Shew_3773 at 4492.840 kb on - strand, within Shew_3773 at 4492.957 kb on + strand, within Shew_3773 at 4493.092 kb on + strand, within Shew_3773 at 4493.223 kb on - strand, within Shew_3773 at 4493.408 kb on - strand, within Shew_3773 at 4493.442 kb on - strand, within Shew_3773 at 4493.590 kb on + strand, within Shew_3773 at 4493.649 kb on + strand at 4493.812 kb on - strand, within Shew_3774 at 4493.819 kb on - strand, within Shew_3774 at 4494.028 kb on - strand, within Shew_3774 at 4494.039 kb on - strand, within Shew_3774 at 4494.039 kb on - strand, within Shew_3774 at 4494.182 kb on + strand, within Shew_3775 at 4494.196 kb on - strand, within Shew_3775 at 4494.461 kb on + strand at 4494.506 kb on + strand at 4494.645 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 4,491,759 - Shew_3771 0.55 -1.6 4,492,059 - -1.2 4,492,155 - +1.6 4,492,632 + Shew_3772 0.87 +0.3 4,492,787 - -0.2 4,492,818 + Shew_3773 0.11 -0.3 4,492,840 - Shew_3773 0.13 -2.4 4,492,957 + Shew_3773 0.24 -0.3 4,493,092 + Shew_3773 0.37 +0.0 4,493,223 - Shew_3773 0.50 -0.7 4,493,408 - Shew_3773 0.68 +0.6 4,493,442 - Shew_3773 0.71 -0.8 4,493,590 + Shew_3773 0.85 -0.8 4,493,649 + +0.2 4,493,812 - Shew_3774 0.14 +0.0 4,493,819 - Shew_3774 0.17 +0.2 4,494,028 - Shew_3774 0.80 -0.5 4,494,039 - Shew_3774 0.84 +0.1 4,494,039 - Shew_3774 0.84 -0.3 4,494,182 + Shew_3775 0.21 +0.6 4,494,196 - Shew_3775 0.24 +1.1 4,494,461 + -0.4 4,494,506 + -0.0 4,494,645 + +0.7
Or see this region's nucleotide sequence