Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2537 and Shew_2538 are separated by 216 nucleotides Shew_2538 and Shew_2539 overlap by 4 nucleotides Shew_2539 and Shew_2540 are separated by 12 nucleotides Shew_2540 and Shew_2541 are separated by 24 nucleotides
Shew_2537: Shew_2537 - AraC family transcriptional regulator (RefSeq), at 2,995,324 to 2,996,262
_2537
Shew_2538: Shew_2538 - AzlC family protein (RefSeq), at 2,996,479 to 2,997,216
_2538
Shew_2539: Shew_2539 - branched-chain amino acid transport (RefSeq), at 2,997,213 to 2,997,518
_2539
Shew_2540: Shew_2540 - NIPSNAP family protein (RefSeq), at 2,997,531 to 2,997,842
_2540
Shew_2541: Shew_2541 - helix-turn-helix, type 11 domain-containing protein (RefSeq), at 2,997,867 to 2,998,553
_2541
Position (kb)
2996
2997
2998 Strain fitness (log2 ratio)
-2
-1
0
1 at 2995.519 kb on - strand, within Shew_2537 at 2995.668 kb on - strand, within Shew_2537 at 2996.405 kb on - strand at 2996.748 kb on + strand, within Shew_2538 at 2996.804 kb on - strand, within Shew_2538 at 2996.960 kb on + strand, within Shew_2538 at 2997.043 kb on - strand, within Shew_2538 at 2997.086 kb on + strand, within Shew_2538 at 2997.148 kb on + strand at 2997.586 kb on - strand, within Shew_2540 at 2997.728 kb on - strand, within Shew_2540 at 2998.045 kb on - strand, within Shew_2541 at 2998.124 kb on + strand, within Shew_2541 at 2998.210 kb on + strand, within Shew_2541
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 2,995,519 - Shew_2537 0.21 +0.1 2,995,668 - Shew_2537 0.37 -2.6 2,996,405 - +0.1 2,996,748 + Shew_2538 0.36 +0.4 2,996,804 - Shew_2538 0.44 +0.0 2,996,960 + Shew_2538 0.65 -0.4 2,997,043 - Shew_2538 0.76 -0.3 2,997,086 + Shew_2538 0.82 -0.2 2,997,148 + +0.5 2,997,586 - Shew_2540 0.18 -0.8 2,997,728 - Shew_2540 0.63 -0.0 2,998,045 - Shew_2541 0.26 -0.2 2,998,124 + Shew_2541 0.37 +0.4 2,998,210 + Shew_2541 0.50 -0.3
Or see this region's nucleotide sequence