Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0974 and Shew_0975 are separated by 10 nucleotides Shew_0975 and Shew_0976 overlap by 4 nucleotides Shew_0976 and Shew_0977 are separated by 83 nucleotides
Shew_0974: Shew_0974 - spermidine/putrescine ABC transporter ATPase subunit (RefSeq), at 1,118,906 to 1,120,042
_0974
Shew_0975: Shew_0975 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 1,120,053 to 1,120,958
_0975
Shew_0976: Shew_0976 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 1,120,955 to 1,121,782
_0976
Shew_0977: Shew_0977 - FAD dependent oxidoreductase (RefSeq), at 1,121,866 to 1,123,152
_0977
Position (kb)
1120
1121 Strain fitness (log2 ratio)
-1
0
1
2 at 1119.146 kb on + strand, within Shew_0974 at 1119.322 kb on + strand, within Shew_0974 at 1119.324 kb on - strand, within Shew_0974 at 1119.663 kb on + strand, within Shew_0974 at 1119.761 kb on + strand, within Shew_0974 at 1119.949 kb on + strand at 1120.091 kb on + strand at 1120.394 kb on + strand, within Shew_0975 at 1120.412 kb on - strand, within Shew_0975 at 1120.586 kb on - strand, within Shew_0975 at 1120.586 kb on - strand, within Shew_0975 at 1120.687 kb on - strand, within Shew_0975 at 1120.803 kb on - strand, within Shew_0975 at 1120.803 kb on - strand, within Shew_0975 at 1120.939 kb on - strand at 1121.154 kb on + strand, within Shew_0976 at 1121.162 kb on - strand, within Shew_0976 at 1121.494 kb on + strand, within Shew_0976 at 1121.790 kb on + strand at 1121.870 kb on + strand at 1121.876 kb on - strand at 1121.878 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 1,119,146 + Shew_0974 0.21 +0.4 1,119,322 + Shew_0974 0.37 +0.5 1,119,324 - Shew_0974 0.37 +0.3 1,119,663 + Shew_0974 0.67 +0.2 1,119,761 + Shew_0974 0.75 +2.5 1,119,949 + -0.5 1,120,091 + -0.8 1,120,394 + Shew_0975 0.38 +0.7 1,120,412 - Shew_0975 0.40 +1.3 1,120,586 - Shew_0975 0.59 +0.3 1,120,586 - Shew_0975 0.59 +0.5 1,120,687 - Shew_0975 0.70 +0.3 1,120,803 - Shew_0975 0.83 -0.9 1,120,803 - Shew_0975 0.83 +0.3 1,120,939 - -0.3 1,121,154 + Shew_0976 0.24 +0.5 1,121,162 - Shew_0976 0.25 +0.2 1,121,494 + Shew_0976 0.65 +0.9 1,121,790 + -0.3 1,121,870 + +0.1 1,121,876 - +0.6 1,121,878 - -0.6
Or see this region's nucleotide sequence