Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0536 and Shew_0537 are separated by 287 nucleotides Shew_0537 and Shew_0538 are separated by 181 nucleotides Shew_0538 and Shew_0539 are separated by 18 nucleotides Shew_0539 and Shew_0540 are separated by 15 nucleotides
Shew_0536: Shew_0536 - tRNA-dihydrouridine synthase A (RefSeq), at 609,087 to 610,076
_0536
Shew_0537: Shew_0537 - hypothetical protein (RefSeq), at 610,364 to 610,642
_0537
Shew_0538: Shew_0538 - phage shock protein C, PspC (RefSeq), at 610,824 to 611,030
_0538
Shew_0539: Shew_0539 - hypothetical protein (RefSeq), at 611,049 to 611,408
_0539
Shew_0540: Shew_0540 - enoyl-CoA hydratase/isomerase (RefSeq), at 611,424 to 612,161
_0540
Position (kb) 610
611 Strain fitness (log2 ratio) -1
0
1
2 at 609.725 kb on + strand, within Shew_0536 at 609.743 kb on - strand, within Shew_0536 at 609.743 kb on - strand, within Shew_0536 at 609.770 kb on - strand, within Shew_0536 at 609.780 kb on - strand, within Shew_0536 at 610.425 kb on - strand, within Shew_0537 at 610.714 kb on - strand at 610.745 kb on + strand at 610.745 kb on + strand at 610.745 kb on + strand at 610.745 kb on + strand at 610.753 kb on - strand at 610.753 kb on - strand at 610.879 kb on + strand, within Shew_0538 at 611.385 kb on + strand at 611.385 kb on + strand at 611.385 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 609,725 + Shew_0536 0.64 +0.4 609,743 - Shew_0536 0.66 +1.7 609,743 - Shew_0536 0.66 +0.2 609,770 - Shew_0536 0.69 +0.2 609,780 - Shew_0536 0.70 +1.6 610,425 - Shew_0537 0.22 -1.0 610,714 - -0.1 610,745 + -0.5 610,745 + +0.4 610,745 + +0.2 610,745 + -0.5 610,753 - +2.2 610,753 - -0.4 610,879 + Shew_0538 0.27 -0.1 611,385 + +0.5 611,385 + -0.3 611,385 + +0.8
Or see this region's nucleotide sequence