Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0441 and Shew_0442 are separated by 133 nucleotides Shew_0442 and Shew_0443 are separated by 173 nucleotides
Shew_0441: Shew_0441 - dehydratase (RefSeq), at 491,155 to 491,994
_0441
Shew_0442: Shew_0442 - aldo/keto reductase (RefSeq), at 492,128 to 493,018
_0442
Shew_0443: Shew_0443 - formate--tetrahydrofolate ligase (RefSeq), at 493,192 to 494,886
_0443
Position (kb)
492
493
494 Strain fitness (log2 ratio)
-2
-1
0
1 at 491.166 kb on + strand at 491.231 kb on + strand at 491.246 kb on + strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.254 kb on - strand, within Shew_0441 at 491.292 kb on + strand, within Shew_0441 at 491.300 kb on - strand, within Shew_0441 at 491.478 kb on - strand, within Shew_0441 at 491.559 kb on + strand, within Shew_0441 at 491.566 kb on - strand, within Shew_0441 at 491.621 kb on + strand, within Shew_0441 at 491.660 kb on + strand, within Shew_0441 at 491.793 kb on + strand, within Shew_0441 at 491.843 kb on - strand, within Shew_0441 at 491.843 kb on - strand, within Shew_0441 at 492.252 kb on - strand, within Shew_0442 at 492.401 kb on + strand, within Shew_0442 at 492.409 kb on - strand, within Shew_0442 at 492.460 kb on + strand, within Shew_0442 at 492.468 kb on - strand, within Shew_0442 at 492.655 kb on + strand, within Shew_0442 at 492.751 kb on + strand, within Shew_0442 at 492.870 kb on + strand, within Shew_0442 at 493.004 kb on + strand at 493.005 kb on - strand at 493.014 kb on + strand at 493.014 kb on + strand at 493.014 kb on + strand at 493.014 kb on + strand at 493.014 kb on + strand at 493.022 kb on - strand at 493.022 kb on - strand at 493.022 kb on - strand at 493.022 kb on - strand at 493.022 kb on - strand at 493.022 kb on - strand at 493.122 kb on - strand at 493.187 kb on + strand at 493.234 kb on - strand at 493.302 kb on + strand at 493.406 kb on - strand, within Shew_0443 at 493.413 kb on - strand, within Shew_0443 at 493.415 kb on + strand, within Shew_0443 at 493.415 kb on + strand, within Shew_0443 at 493.423 kb on - strand, within Shew_0443 at 493.590 kb on + strand, within Shew_0443 at 493.774 kb on - strand, within Shew_0443 at 493.975 kb on + strand, within Shew_0443
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 491,166 + -1.2 491,231 + -1.2 491,246 + Shew_0441 0.11 -1.1 491,254 - Shew_0441 0.12 -0.2 491,254 - Shew_0441 0.12 -1.5 491,254 - Shew_0441 0.12 -1.0 491,254 - Shew_0441 0.12 -1.3 491,254 - Shew_0441 0.12 -1.1 491,292 + Shew_0441 0.16 -1.2 491,300 - Shew_0441 0.17 -1.2 491,478 - Shew_0441 0.38 -1.7 491,559 + Shew_0441 0.48 -1.1 491,566 - Shew_0441 0.49 +0.1 491,621 + Shew_0441 0.55 -1.3 491,660 + Shew_0441 0.60 -0.7 491,793 + Shew_0441 0.76 -1.1 491,843 - Shew_0441 0.82 -0.8 491,843 - Shew_0441 0.82 -1.9 492,252 - Shew_0442 0.14 -0.3 492,401 + Shew_0442 0.31 -0.6 492,409 - Shew_0442 0.32 -0.1 492,460 + Shew_0442 0.37 +0.1 492,468 - Shew_0442 0.38 -0.7 492,655 + Shew_0442 0.59 -0.7 492,751 + Shew_0442 0.70 -0.1 492,870 + Shew_0442 0.83 -0.3 493,004 + +0.5 493,005 - +0.0 493,014 + +0.4 493,014 + -0.0 493,014 + +0.2 493,014 + +0.1 493,014 + -0.3 493,022 - +0.6 493,022 - +0.2 493,022 - -0.6 493,022 - +0.0 493,022 - +0.8 493,022 - +0.3 493,122 - -0.8 493,187 + -0.1 493,234 - -1.1 493,302 + +0.2 493,406 - Shew_0443 0.13 -0.1 493,413 - Shew_0443 0.13 +0.0 493,415 + Shew_0443 0.13 +0.8 493,415 + Shew_0443 0.13 +0.5 493,423 - Shew_0443 0.14 +0.7 493,590 + Shew_0443 0.23 +0.3 493,774 - Shew_0443 0.34 +0.2 493,975 + Shew_0443 0.46 +0.1
Or see this region's nucleotide sequence