Experiment: Paraquat dichloride 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SOA0083 and SO_A0180 are separated by 500 nucleotides SO_A0180 and SOA0085 are separated by 336 nucleotides SOA0085 and SOA0086 are separated by 83 nucleotides
SO_A0084: SO_A0084 - no description, at 62,973 to 64,960
_A0084
SOA0083: SOA0083 - ISSod4, transposase (NCBI ptt file), at 63,339 to 64,541
SOA0083
SO_A0180: SO_A0180 - hypothetical protein (NCBI), at 65,042 to 65,569
_A0180
SOA0085: SOA0085 - ISSod8, transposase (NCBI ptt file), at 65,906 to 66,451
SOA0085
SOA0086: SOA0086 - site-specific recombinase, resolvase family (NCBI ptt file), at 66,535 to 67,125
SOA0086
Position (kb)
65
66 Strain fitness (log2 ratio)
-1
0
1 at 64.560 kb on + strand at 64.599 kb on - strand at 64.600 kb on + strand at 64.613 kb on - strand at 64.619 kb on + strand at 64.619 kb on + strand at 64.619 kb on + strand at 64.627 kb on - strand at 64.627 kb on - strand at 64.684 kb on - strand at 64.730 kb on + strand at 64.730 kb on + strand at 64.738 kb on - strand at 64.738 kb on - strand at 64.743 kb on + strand at 64.751 kb on - strand at 64.780 kb on + strand at 64.787 kb on - strand at 64.787 kb on - strand at 64.787 kb on - strand at 64.812 kb on + strand at 64.855 kb on + strand at 64.863 kb on - strand at 64.865 kb on - strand at 64.865 kb on - strand at 64.882 kb on + strand at 64.900 kb on + strand at 64.912 kb on - strand at 64.939 kb on + strand at 64.955 kb on - strand at 64.963 kb on - strand at 65.011 kb on + strand at 65.011 kb on + strand at 65.019 kb on - strand at 65.019 kb on - strand at 65.064 kb on + strand at 65.065 kb on - strand at 65.065 kb on - strand at 65.096 kb on - strand, within SO_A0180 at 65.125 kb on + strand, within SO_A0180 at 65.125 kb on + strand, within SO_A0180 at 65.125 kb on + strand, within SO_A0180 at 65.132 kb on - strand, within SO_A0180 at 65.133 kb on - strand, within SO_A0180 at 65.133 kb on - strand, within SO_A0180 at 65.133 kb on - strand, within SO_A0180 at 65.138 kb on - strand, within SO_A0180 at 65.178 kb on + strand, within SO_A0180 at 65.179 kb on + strand, within SO_A0180 at 65.186 kb on - strand, within SO_A0180 at 65.190 kb on + strand, within SO_A0180 at 65.204 kb on - strand, within SO_A0180 at 65.209 kb on - strand, within SO_A0180 at 65.209 kb on - strand, within SO_A0180 at 65.221 kb on + strand, within SO_A0180 at 65.229 kb on - strand, within SO_A0180 at 65.231 kb on + strand, within SO_A0180 at 65.257 kb on + strand, within SO_A0180 at 65.264 kb on + strand, within SO_A0180 at 65.294 kb on + strand, within SO_A0180 at 65.294 kb on + strand, within SO_A0180 at 65.302 kb on - strand, within SO_A0180 at 65.302 kb on - strand, within SO_A0180 at 65.309 kb on + strand, within SO_A0180 at 65.357 kb on + strand, within SO_A0180 at 65.367 kb on + strand, within SO_A0180 at 65.375 kb on + strand, within SO_A0180 at 65.407 kb on - strand, within SO_A0180 at 65.421 kb on + strand, within SO_A0180 at 65.421 kb on + strand, within SO_A0180 at 65.421 kb on + strand, within SO_A0180 at 65.429 kb on - strand, within SO_A0180 at 65.451 kb on - strand, within SO_A0180 at 65.451 kb on - strand, within SO_A0180 at 65.461 kb on - strand, within SO_A0180 at 65.461 kb on - strand, within SO_A0180 at 65.521 kb on + strand at 65.521 kb on + strand at 65.524 kb on - strand at 65.526 kb on + strand at 65.526 kb on - strand at 65.529 kb on - strand at 65.529 kb on - strand at 65.531 kb on - strand at 65.532 kb on - strand at 65.537 kb on - strand at 65.537 kb on - strand at 65.595 kb on + strand at 65.608 kb on - strand at 65.614 kb on + strand at 65.614 kb on + strand at 65.622 kb on + strand at 65.622 kb on - strand at 65.622 kb on - strand at 65.622 kb on - strand at 65.624 kb on + strand at 65.624 kb on + strand at 65.631 kb on - strand at 65.632 kb on - strand at 65.632 kb on - strand at 65.676 kb on + strand at 65.676 kb on + strand at 65.685 kb on + strand at 65.690 kb on + strand at 66.278 kb on - strand, within SOA0085 at 66.511 kb on + strand at 66.542 kb on + strand at 66.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.25 mg/ml remove 64,560 + +0.6 64,599 - +0.3 64,600 + +0.5 64,613 - +0.3 64,619 + +0.4 64,619 + -0.8 64,619 + -0.4 64,627 - +0.3 64,627 - +0.4 64,684 - +0.3 64,730 + +0.2 64,730 + +0.5 64,738 - +0.2 64,738 - -0.3 64,743 + -0.0 64,751 - -0.4 64,780 + -0.3 64,787 - +0.2 64,787 - -0.3 64,787 - -0.9 64,812 + -1.1 64,855 + +0.2 64,863 - +0.1 64,865 - +0.2 64,865 - -0.5 64,882 + -0.5 64,900 + -0.4 64,912 - -0.3 64,939 + +0.6 64,955 - +0.5 64,963 - -0.4 65,011 + +0.0 65,011 + +0.0 65,019 - -0.8 65,019 - +1.4 65,064 + +0.2 65,065 - +0.5 65,065 - +0.4 65,096 - SO_A0180 0.10 +0.9 65,125 + SO_A0180 0.16 +0.1 65,125 + SO_A0180 0.16 +0.3 65,125 + SO_A0180 0.16 -0.1 65,132 - SO_A0180 0.17 +0.3 65,133 - SO_A0180 0.17 +0.2 65,133 - SO_A0180 0.17 +0.6 65,133 - SO_A0180 0.17 +0.4 65,138 - SO_A0180 0.18 -0.2 65,178 + SO_A0180 0.26 +0.7 65,179 + SO_A0180 0.26 +0.8 65,186 - SO_A0180 0.27 +0.8 65,190 + SO_A0180 0.28 +0.2 65,204 - SO_A0180 0.31 +0.2 65,209 - SO_A0180 0.32 +0.4 65,209 - SO_A0180 0.32 +0.7 65,221 + SO_A0180 0.34 +0.2 65,229 - SO_A0180 0.35 +0.3 65,231 + SO_A0180 0.36 +1.0 65,257 + SO_A0180 0.41 -0.4 65,264 + SO_A0180 0.42 +0.2 65,294 + SO_A0180 0.48 -0.1 65,294 + SO_A0180 0.48 +1.4 65,302 - SO_A0180 0.49 +0.3 65,302 - SO_A0180 0.49 +0.9 65,309 + SO_A0180 0.51 +0.7 65,357 + SO_A0180 0.60 +0.9 65,367 + SO_A0180 0.62 +0.6 65,375 + SO_A0180 0.63 -0.5 65,407 - SO_A0180 0.69 -0.2 65,421 + SO_A0180 0.72 -0.1 65,421 + SO_A0180 0.72 -0.2 65,421 + SO_A0180 0.72 +0.0 65,429 - SO_A0180 0.73 -0.7 65,451 - SO_A0180 0.77 -0.5 65,451 - SO_A0180 0.77 -0.6 65,461 - SO_A0180 0.79 +0.4 65,461 - SO_A0180 0.79 +1.6 65,521 + +0.2 65,521 + +1.0 65,524 - -0.2 65,526 + +0.0 65,526 - +0.1 65,529 - -0.1 65,529 - -0.4 65,531 - +0.4 65,532 - -0.0 65,537 - -0.4 65,537 - +0.1 65,595 + +0.3 65,608 - +0.2 65,614 + -1.1 65,614 + +1.2 65,622 + -0.3 65,622 - +1.0 65,622 - +1.0 65,622 - -0.5 65,624 + -0.8 65,624 + +0.2 65,631 - -0.3 65,632 - +0.7 65,632 - +0.2 65,676 + +0.2 65,676 + -1.0 65,685 + -0.8 65,690 + -0.2 66,278 - SOA0085 0.68 +0.3 66,511 + +1.4 66,542 + +0.0 66,564 + -0.0
Or see this region's nucleotide sequence