Experiment: Paraquat dichloride 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutY and SO3369 are separated by 9 nucleotides SO3369 and SO3370 are separated by 80 nucleotides SO3370 and SO3371 are separated by 4 nucleotides
SO3368: mutY - A/G-specific adenine glycosylase (NCBI ptt file), at 3,510,656 to 3,511,753
mutY
SO3369: SO3369 - conserved hypothetical protein (NCBI ptt file), at 3,511,763 to 3,512,041
SO3369
SO3370: SO3370 - conserved hypothetical protein (NCBI ptt file), at 3,512,122 to 3,512,697
SO3370
SO3371: SO3371 - hypothetical Cytochrome B561 (NCBI ptt file), at 3,512,702 to 3,513,253
SO3371
Position (kb)
3511
3512
3513 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3510.790 kb on + strand, within mutY at 3510.901 kb on - strand, within mutY at 3510.925 kb on + strand, within mutY at 3510.978 kb on + strand, within mutY at 3511.004 kb on + strand, within mutY at 3511.004 kb on + strand, within mutY at 3511.004 kb on + strand, within mutY at 3511.004 kb on + strand, within mutY at 3511.012 kb on - strand, within mutY at 3511.012 kb on - strand, within mutY at 3511.012 kb on - strand, within mutY at 3511.012 kb on - strand, within mutY at 3511.031 kb on + strand, within mutY at 3511.109 kb on + strand, within mutY at 3511.122 kb on - strand, within mutY at 3511.297 kb on - strand, within mutY at 3511.372 kb on + strand, within mutY at 3511.470 kb on + strand, within mutY at 3511.596 kb on + strand, within mutY at 3511.882 kb on - strand, within SO3369 at 3511.904 kb on + strand, within SO3369 at 3511.992 kb on + strand, within SO3369 at 3512.048 kb on + strand at 3512.056 kb on - strand at 3512.056 kb on - strand at 3512.157 kb on - strand at 3512.163 kb on + strand at 3512.163 kb on + strand at 3512.170 kb on + strand at 3512.249 kb on + strand, within SO3370 at 3512.257 kb on - strand, within SO3370 at 3512.263 kb on + strand, within SO3370 at 3512.402 kb on + strand, within SO3370 at 3512.428 kb on - strand, within SO3370 at 3512.495 kb on + strand, within SO3370 at 3512.495 kb on + strand, within SO3370 at 3512.509 kb on - strand, within SO3370 at 3512.509 kb on - strand, within SO3370 at 3512.560 kb on + strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.568 kb on - strand, within SO3370 at 3512.587 kb on + strand, within SO3370 at 3512.671 kb on - strand at 3512.732 kb on - strand at 3512.734 kb on + strand at 3512.734 kb on + strand at 3512.742 kb on - strand at 3512.786 kb on - strand, within SO3371 at 3512.804 kb on + strand, within SO3371 at 3512.804 kb on + strand, within SO3371 at 3512.825 kb on + strand, within SO3371 at 3512.871 kb on - strand, within SO3371 at 3512.904 kb on - strand, within SO3371 at 3512.986 kb on - strand, within SO3371 at 3513.038 kb on - strand, within SO3371
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.25 mg/ml remove 3,510,790 + mutY SO3368 0.12 -0.9 3,510,901 - mutY SO3368 0.22 -0.3 3,510,925 + mutY SO3368 0.24 -0.8 3,510,978 + mutY SO3368 0.29 -0.8 3,511,004 + mutY SO3368 0.32 -0.9 3,511,004 + mutY SO3368 0.32 +0.0 3,511,004 + mutY SO3368 0.32 +0.8 3,511,004 + mutY SO3368 0.32 -0.1 3,511,012 - mutY SO3368 0.32 -0.2 3,511,012 - mutY SO3368 0.32 +0.4 3,511,012 - mutY SO3368 0.32 +0.3 3,511,012 - mutY SO3368 0.32 -0.9 3,511,031 + mutY SO3368 0.34 +0.0 3,511,109 + mutY SO3368 0.41 -0.4 3,511,122 - mutY SO3368 0.42 -0.1 3,511,297 - mutY SO3368 0.58 -0.4 3,511,372 + mutY SO3368 0.65 -0.2 3,511,470 + mutY SO3368 0.74 -0.7 3,511,596 + mutY SO3368 0.86 -0.5 3,511,882 - SO3369 0.43 -2.7 3,511,904 + SO3369 0.51 -2.6 3,511,992 + SO3369 0.82 -3.5 3,512,048 + -0.9 3,512,056 - +1.0 3,512,056 - -1.9 3,512,157 - +0.3 3,512,163 + +1.3 3,512,163 + -0.6 3,512,170 + +0.3 3,512,249 + SO3370 0.22 +1.0 3,512,257 - SO3370 0.23 -0.5 3,512,263 + SO3370 0.24 +0.3 3,512,402 + SO3370 0.49 +1.4 3,512,428 - SO3370 0.53 -0.8 3,512,495 + SO3370 0.65 +0.1 3,512,495 + SO3370 0.65 +0.2 3,512,509 - SO3370 0.67 -0.5 3,512,509 - SO3370 0.67 +1.2 3,512,560 + SO3370 0.76 -1.5 3,512,568 - SO3370 0.77 -0.2 3,512,568 - SO3370 0.77 -0.8 3,512,568 - SO3370 0.77 -0.9 3,512,568 - SO3370 0.77 -0.6 3,512,568 - SO3370 0.77 -0.5 3,512,587 + SO3370 0.81 -1.1 3,512,671 - +0.1 3,512,732 - -0.1 3,512,734 + -1.0 3,512,734 + +0.5 3,512,742 - +0.1 3,512,786 - SO3371 0.15 -0.5 3,512,804 + SO3371 0.18 +1.1 3,512,804 + SO3371 0.18 +0.4 3,512,825 + SO3371 0.22 +0.4 3,512,871 - SO3371 0.31 +0.6 3,512,904 - SO3371 0.37 +0.1 3,512,986 - SO3371 0.51 -1.1 3,513,038 - SO3371 0.61 -0.4
Or see this region's nucleotide sequence