Experiment: Paraquat dichloride 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ccmE and ccmD overlap by 4 nucleotides ccmD and ccmC overlap by 1 nucleotides ccmC and ccmB are separated by 1 nucleotides ccmB and ccmA are separated by 11 nucleotides
SO0259: ccmE - cytochrome c biogenesis protein CcmE (NCBI ptt file), at 260,541 to 261,026
ccmE
SO0260: ccmD - heme exporter protein CcmD (NCBI ptt file), at 261,023 to 261,223
ccmD
SO0261: ccmC - heme exporter protein CcmC (NCBI ptt file), at 261,223 to 261,969
ccmC
SO0262: ccmB - heme exporter protein CcmB (NCBI ptt file), at 261,971 to 262,657
ccmB
SO0263: ccmA - heme exporter protein CcmA (NCBI ptt file), at 262,669 to 263,319
ccmA
Position (kb) 261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.430 kb on + strand at 260.471 kb on - strand at 260.646 kb on + strand, within ccmE at 260.821 kb on - strand, within ccmE at 260.858 kb on - strand, within ccmE at 261.000 kb on - strand at 261.581 kb on - strand, within ccmC at 261.600 kb on + strand, within ccmC at 261.638 kb on + strand, within ccmC at 261.801 kb on - strand, within ccmC at 261.952 kb on - strand at 262.012 kb on + strand at 262.226 kb on + strand, within ccmB at 262.372 kb on + strand, within ccmB at 262.377 kb on + strand, within ccmB at 262.401 kb on - strand, within ccmB at 262.641 kb on - strand at 262.666 kb on + strand at 262.668 kb on - strand at 262.876 kb on - strand, within ccmA at 262.899 kb on - strand, within ccmA
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.25 mg/ml remove 260,430 + +0.4 260,471 - +0.5 260,646 + ccmE SO0259 0.22 -0.9 260,821 - ccmE SO0259 0.58 -1.2 260,858 - ccmE SO0259 0.65 -0.3 261,000 - -1.3 261,581 - ccmC SO0261 0.48 -1.7 261,600 + ccmC SO0261 0.50 -1.9 261,638 + ccmC SO0261 0.56 -3.1 261,801 - ccmC SO0261 0.77 -2.3 261,952 - -0.9 262,012 + -1.2 262,226 + ccmB SO0262 0.37 -1.7 262,372 + ccmB SO0262 0.58 -2.9 262,377 + ccmB SO0262 0.59 -1.3 262,401 - ccmB SO0262 0.63 -2.4 262,641 - -0.5 262,666 + -1.7 262,668 - +0.9 262,876 - ccmA SO0263 0.32 -1.0 262,899 - ccmA SO0263 0.35 -1.3
Or see this region's nucleotide sequence