Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaR and Shewana3_3037 are separated by 95 nucleotides Shewana3_3037 and Shewana3_3038 are separated by 2 nucleotides Shewana3_3038 and thyA are separated by 118 nucleotides thyA and Shewana3_3040 overlap by 1 nucleotides
Shewana3_3036: nhaR - transcriptional activator NhaR (RefSeq), at 3,640,469 to 3,641,377
nhaR
Shewana3_3037: Shewana3_3037 - hypothetical protein (RefSeq), at 3,641,473 to 3,641,859
_3037
Shewana3_3038: Shewana3_3038 - Na+/H+ antiporter NhaA (RefSeq), at 3,641,862 to 3,643,031
_3038
Shewana3_3039: thyA - thymidylate synthase (RefSeq), at 3,643,150 to 3,643,944
thyA
Shewana3_3040: Shewana3_3040 - prolipoprotein diacylglyceryl transferase (RefSeq), at 3,643,944 to 3,644,750
_3040
Position (kb)
3641
3642
3643
3644 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3640.865 kb on + strand, within nhaR at 3641.137 kb on + strand, within nhaR at 3641.276 kb on + strand, within nhaR at 3641.290 kb on + strand at 3641.298 kb on - strand at 3641.305 kb on - strand at 3641.411 kb on + strand at 3641.421 kb on + strand at 3641.428 kb on + strand at 3641.647 kb on + strand, within Shewana3_3037 at 3641.665 kb on - strand, within Shewana3_3037 at 3641.665 kb on - strand, within Shewana3_3037 at 3641.929 kb on - strand at 3641.961 kb on + strand at 3642.180 kb on - strand, within Shewana3_3038 at 3642.264 kb on + strand, within Shewana3_3038 at 3642.369 kb on + strand, within Shewana3_3038 at 3642.375 kb on - strand, within Shewana3_3038 at 3642.438 kb on + strand, within Shewana3_3038 at 3642.446 kb on - strand, within Shewana3_3038 at 3642.446 kb on - strand, within Shewana3_3038 at 3642.775 kb on + strand, within Shewana3_3038 at 3642.836 kb on - strand, within Shewana3_3038 at 3642.853 kb on + strand, within Shewana3_3038
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 3,640,865 + nhaR Shewana3_3036 0.44 +0.8 3,641,137 + nhaR Shewana3_3036 0.73 -1.6 3,641,276 + nhaR Shewana3_3036 0.89 +0.3 3,641,290 + +0.5 3,641,298 - -2.4 3,641,305 - -2.0 3,641,411 + +0.7 3,641,421 + -0.8 3,641,428 + +0.4 3,641,647 + Shewana3_3037 0.45 +1.0 3,641,665 - Shewana3_3037 0.50 -3.6 3,641,665 - Shewana3_3037 0.50 +0.4 3,641,929 - -0.4 3,641,961 + +0.7 3,642,180 - Shewana3_3038 0.27 +0.3 3,642,264 + Shewana3_3038 0.34 -0.8 3,642,369 + Shewana3_3038 0.43 +0.0 3,642,375 - Shewana3_3038 0.44 +0.4 3,642,438 + Shewana3_3038 0.49 +0.1 3,642,446 - Shewana3_3038 0.50 -1.0 3,642,446 - Shewana3_3038 0.50 -1.7 3,642,775 + Shewana3_3038 0.78 -1.9 3,642,836 - Shewana3_3038 0.83 +0.3 3,642,853 + Shewana3_3038 0.85 -0.9
Or see this region's nucleotide sequence