Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3023 and Shewana3_3024 are separated by 89 nucleotides Shewana3_3024 and era are separated by 49 nucleotides era and rnc overlap by 4 nucleotides rnc and Shewana3_3027 are separated by 1 nucleotides
Shewana3_3023: Shewana3_3023 - pyridoxine 5'-phosphate synthase (RefSeq), at 3,628,834 to 3,629,571
_3023
Shewana3_3024: Shewana3_3024 - DNA repair protein RecO (RefSeq), at 3,629,661 to 3,630,362
_3024
Shewana3_3025: era - GTP-binding protein Era (RefSeq), at 3,630,412 to 3,631,431
era
Shewana3_3026: rnc - ribonuclease III (RefSeq), at 3,631,428 to 3,632,108
rnc
Shewana3_3027: Shewana3_3027 - signal peptidase I (RefSeq), at 3,632,110 to 3,633,027
_3027
Position (kb)
3630
3631
3632 Strain fitness (log2 ratio)
-2
-1
0
1 at 3629.629 kb on - strand at 3629.629 kb on - strand at 3629.840 kb on + strand, within Shewana3_3024 at 3629.975 kb on + strand, within Shewana3_3024 at 3629.983 kb on - strand, within Shewana3_3024 at 3629.983 kb on - strand, within Shewana3_3024 at 3629.990 kb on + strand, within Shewana3_3024 at 3630.066 kb on - strand, within Shewana3_3024 at 3630.187 kb on - strand, within Shewana3_3024 at 3630.328 kb on - strand at 3630.409 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 3,629,629 - -1.2 3,629,629 - -0.7 3,629,840 + Shewana3_3024 0.25 -0.8 3,629,975 + Shewana3_3024 0.45 +0.3 3,629,983 - Shewana3_3024 0.46 -2.3 3,629,983 - Shewana3_3024 0.46 -0.9 3,629,990 + Shewana3_3024 0.47 +0.3 3,630,066 - Shewana3_3024 0.58 -1.9 3,630,187 - Shewana3_3024 0.75 -2.2 3,630,328 - +0.1 3,630,409 + -1.3
Or see this region's nucleotide sequence