Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsf and rpsB are separated by 119 nucleotides rpsB and Shewana3_R0115 are separated by 23 nucleotides Shewana3_R0115 and Shewana3_2816 are separated by 94 nucleotides
Shewana3_2814: tsf - elongation factor Ts (RefSeq), at 3,360,814 to 3,361,665
tsf
Shewana3_2815: rpsB - 30S ribosomal protein S2 (RefSeq), at 3,361,785 to 3,362,513
rpsB
Shewana3_R0115: Shewana3_R0115 - t44 RNA (NCBI), at 3,362,537 to 3,362,688
_R0115
Shewana3_2816: Shewana3_2816 - methionine aminopeptidase (RefSeq), at 3,362,783 to 3,363,619
_2816
Position (kb)
3361
3362
3363 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3362.841 kb on - strand at 3362.870 kb on + strand, within Shewana3_2816 at 3362.923 kb on - strand, within Shewana3_2816 at 3362.942 kb on - strand, within Shewana3_2816 at 3363.218 kb on - strand, within Shewana3_2816 at 3363.244 kb on + strand, within Shewana3_2816 at 3363.407 kb on + strand, within Shewana3_2816 at 3363.440 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.470 kb on + strand, within Shewana3_2816 at 3363.478 kb on - strand, within Shewana3_2816 at 3363.478 kb on - strand, within Shewana3_2816
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 3,362,841 - -2.5 3,362,870 + Shewana3_2816 0.10 -2.4 3,362,923 - Shewana3_2816 0.17 -2.4 3,362,942 - Shewana3_2816 0.19 -2.3 3,363,218 - Shewana3_2816 0.52 -3.0 3,363,244 + Shewana3_2816 0.55 -3.2 3,363,407 + Shewana3_2816 0.75 -2.9 3,363,440 + Shewana3_2816 0.78 -2.7 3,363,470 + Shewana3_2816 0.82 -1.4 3,363,470 + Shewana3_2816 0.82 -2.9 3,363,470 + Shewana3_2816 0.82 -3.6 3,363,470 + Shewana3_2816 0.82 -3.7 3,363,470 + Shewana3_2816 0.82 -1.8 3,363,470 + Shewana3_2816 0.82 -2.7 3,363,470 + Shewana3_2816 0.82 -2.4 3,363,478 - Shewana3_2816 0.83 -4.0 3,363,478 - Shewana3_2816 0.83 -0.4
Or see this region's nucleotide sequence