Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2657 and Shewana3_2658 are separated by 226 nucleotides Shewana3_2658 and Shewana3_2659 are separated by 260 nucleotides Shewana3_2659 and clpX are separated by 132 nucleotides
Shewana3_2657: Shewana3_2657 - PpiC-type peptidyl-prolyl cis-trans isomerase (RefSeq), at 3,170,350 to 3,172,215
_2657
Shewana3_2658: Shewana3_2658 - histone family protein DNA-binding protein (RefSeq), at 3,172,442 to 3,172,714
_2658
Shewana3_2659: Shewana3_2659 - Lon-A peptidase (RefSeq), at 3,172,975 to 3,175,332
_2659
Shewana3_2660: clpX - ATP-dependent protease ATP-binding subunit ClpX (RefSeq), at 3,175,465 to 3,176,745
clpX
Position (kb) 3172
3173
3174
3175
3176 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3172.025 kb on - strand, within Shewana3_2657 at 3172.897 kb on + strand at 3172.905 kb on - strand at 3173.565 kb on - strand, within Shewana3_2659 at 3175.879 kb on + strand, within clpX at 3175.924 kb on - strand, within clpX at 3175.924 kb on - strand, within clpX at 3175.924 kb on - strand, within clpX at 3176.073 kb on - strand, within clpX
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 3,172,025 - Shewana3_2657 0.90 -0.7 3,172,897 + -2.0 3,172,905 - -0.4 3,173,565 - Shewana3_2659 0.25 +0.2 3,175,879 + clpX Shewana3_2660 0.32 -1.8 3,175,924 - clpX Shewana3_2660 0.36 -4.2 3,175,924 - clpX Shewana3_2660 0.36 -4.4 3,175,924 - clpX Shewana3_2660 0.36 -2.0 3,176,073 - clpX Shewana3_2660 0.47 -0.7
Or see this region's nucleotide sequence