Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2162 and Shewana3_R0092 are separated by 125 nucleotides Shewana3_R0092 and Shewana3_R0093 are separated by 39 nucleotides Shewana3_R0093 and Shewana3_R0094 are separated by 130 nucleotides Shewana3_R0094 and Shewana3_2163 are separated by 195 nucleotides
Shewana3_2162: Shewana3_2162 - hypothetical protein (RefSeq), at 2,590,896 to 2,591,384
_2162
Shewana3_R0092: Shewana3_R0092 - tRNA-Tyr (RefSeq), at 2,591,510 to 2,591,594
_R0092
Shewana3_R0093: Shewana3_R0093 - tRNA-Tyr (RefSeq), at 2,591,634 to 2,591,718
_R0093
Shewana3_R0094: Shewana3_R0094 - tRNA-Tyr (RefSeq), at 2,591,849 to 2,591,933
_R0094
Shewana3_2163: Shewana3_2163 - radical activating enzyme (RefSeq), at 2,592,129 to 2,592,797
_2163
Position (kb)
2591
2592 Strain fitness (log2 ratio)
-1
0
1 at 2592.094 kb on + strand at 2592.367 kb on - strand, within Shewana3_2163 at 2592.513 kb on - strand, within Shewana3_2163 at 2592.556 kb on + strand, within Shewana3_2163 at 2592.627 kb on + strand, within Shewana3_2163 at 2592.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,592,094 + +0.6 2,592,367 - Shewana3_2163 0.36 +0.6 2,592,513 - Shewana3_2163 0.57 +0.1 2,592,556 + Shewana3_2163 0.64 -1.4 2,592,627 + Shewana3_2163 0.74 -0.7 2,592,920 - -0.1
Or see this region's nucleotide sequence