Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2041 and Shewana3_2042 are separated by 221 nucleotides Shewana3_2042 and Shewana3_2043 are separated by 155 nucleotides Shewana3_2043 and infC are separated by 114 nucleotides infC and rpmI are separated by 85 nucleotides rpmI and rplT are separated by 16 nucleotides
Shewana3_2041: Shewana3_2041 - hypothetical protein (RefSeq), at 2,450,212 to 2,450,412
_2041
Shewana3_2042: Shewana3_2042 - no description, at 2,450,634 to 2,451,113
_2042
Shewana3_2043: Shewana3_2043 - threonyl-tRNA synthetase (RefSeq), at 2,451,269 to 2,453,197
_2043
Shewana3_2044: infC - translation initiation factor IF-3 (RefSeq), at 2,453,312 to 2,453,743
infC
Shewana3_2045: rpmI - 50S ribosomal protein L35 (RefSeq), at 2,453,829 to 2,454,023
rpmI
Shewana3_2046: rplT - 50S ribosomal protein L20 (RefSeq), at 2,454,040 to 2,454,396
rplT
Position (kb)
2451
2452
2453
2454 Strain fitness (log2 ratio)
-1
0
1
2 at 2450.303 kb on - strand, within Shewana3_2041 at 2450.422 kb on - strand at 2450.473 kb on + strand at 2450.479 kb on - strand at 2450.584 kb on + strand at 2450.584 kb on + strand at 2450.679 kb on + strand at 2450.692 kb on - strand, within Shewana3_2042 at 2450.772 kb on + strand, within Shewana3_2042 at 2450.782 kb on + strand, within Shewana3_2042 at 2450.790 kb on - strand, within Shewana3_2042 at 2450.892 kb on - strand at 2450.989 kb on + strand, within Shewana3_2042 at 2450.997 kb on - strand, within Shewana3_2042 at 2451.089 kb on + strand at 2451.178 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,450,303 - Shewana3_2041 0.45 +0.5 2,450,422 - -0.8 2,450,473 + +0.1 2,450,479 - -0.0 2,450,584 + +1.1 2,450,584 + +0.5 2,450,679 + -0.3 2,450,692 - Shewana3_2042 0.12 +0.4 2,450,772 + Shewana3_2042 0.29 +0.7 2,450,782 + Shewana3_2042 0.31 +1.3 2,450,790 - Shewana3_2042 0.33 -0.2 2,450,892 - +0.0 2,450,989 + Shewana3_2042 0.74 -0.7 2,450,997 - Shewana3_2042 0.76 +0.5 2,451,089 + +0.1 2,451,178 + +2.3
Or see this region's nucleotide sequence