Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1903 and Shewana3_1904 are separated by 73 nucleotides Shewana3_1904 and Shewana3_1905 are separated by 360 nucleotides Shewana3_1905 and Shewana3_1906 are separated by 28 nucleotides Shewana3_1906 and Shewana3_1907 are separated by 82 nucleotides
Shewana3_1903: Shewana3_1903 - restriction endonuclease (RefSeq), at 2,259,683 to 2,261,605
_1903
Shewana3_1904: Shewana3_1904 - GCN5-related N-acetyltransferase (RefSeq), at 2,261,679 to 2,262,101
_1904
Shewana3_1905: Shewana3_1905 - hypothetical protein (RefSeq), at 2,262,462 to 2,262,698
_1905
Shewana3_1906: Shewana3_1906 - 3-deoxy-manno-octulosonate cytidylyltransferase (RefSeq), at 2,262,727 to 2,263,464
_1906
Shewana3_1907: Shewana3_1907 - iron-containing alcohol dehydrogenase (RefSeq), at 2,263,547 to 2,264,617
_1907
Position (kb)
2262
2263 Strain fitness (log2 ratio)
-1
0
1 at 2261.635 kb on + strand at 2261.636 kb on + strand at 2261.744 kb on + strand, within Shewana3_1904 at 2262.050 kb on - strand, within Shewana3_1904 at 2262.098 kb on - strand at 2262.161 kb on - strand at 2262.245 kb on - strand at 2262.385 kb on + strand at 2262.646 kb on - strand, within Shewana3_1905 at 2262.745 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,261,635 + +0.2 2,261,636 + -1.7 2,261,744 + Shewana3_1904 0.15 +0.6 2,262,050 - Shewana3_1904 0.88 +0.3 2,262,098 - +0.0 2,262,161 - +0.7 2,262,245 - -0.4 2,262,385 + -1.3 2,262,646 - Shewana3_1905 0.78 -0.2 2,262,745 - -0.7
Or see this region's nucleotide sequence