Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1666 and Shewana3_1667 are separated by 109 nucleotides Shewana3_1667 and Shewana3_1668 are separated by 68 nucleotides Shewana3_1668 and Shewana3_1669 are separated by 0 nucleotides
Shewana3_1666: Shewana3_1666 - butyryl-CoA:acetate CoA transferase (RefSeq), at 1,957,549 to 1,958,205
_1666
Shewana3_1667: Shewana3_1667 - 3-oxoacid CoA-transferase, A subunit (RefSeq), at 1,958,315 to 1,959,019
_1667
Shewana3_1668: Shewana3_1668 - hydroxymethylglutaryl-CoA lyase (RefSeq), at 1,959,088 to 1,960,020
_1668
Shewana3_1669: Shewana3_1669 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit (RefSeq), at 1,960,021 to 1,962,045
_1669
Position (kb)
1959
1960
1961 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1958.358 kb on + strand at 1959.085 kb on + strand at 1959.093 kb on - strand at 1959.093 kb on - strand at 1959.131 kb on - strand at 1959.669 kb on + strand, within Shewana3_1668 at 1959.969 kb on + strand at 1959.969 kb on + strand at 1960.070 kb on - strand at 1960.107 kb on + strand at 1960.216 kb on + strand at 1960.303 kb on - strand, within Shewana3_1669 at 1960.524 kb on + strand, within Shewana3_1669 at 1960.554 kb on + strand, within Shewana3_1669 at 1960.581 kb on - strand, within Shewana3_1669 at 1960.670 kb on + strand, within Shewana3_1669 at 1960.699 kb on - strand, within Shewana3_1669 at 1960.709 kb on + strand, within Shewana3_1669
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,958,358 + +1.1 1,959,085 + +1.6 1,959,093 - +0.4 1,959,093 - -1.0 1,959,131 - +0.7 1,959,669 + Shewana3_1668 0.62 +2.7 1,959,969 + +3.4 1,959,969 + +4.5 1,960,070 - -0.9 1,960,107 + -0.1 1,960,216 + +0.7 1,960,303 - Shewana3_1669 0.14 +0.1 1,960,524 + Shewana3_1669 0.25 -0.0 1,960,554 + Shewana3_1669 0.26 -0.6 1,960,581 - Shewana3_1669 0.28 -1.4 1,960,670 + Shewana3_1669 0.32 +0.3 1,960,699 - Shewana3_1669 0.33 +0.7 1,960,709 + Shewana3_1669 0.34 -0.4
Or see this region's nucleotide sequence