Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secF and Shewana3_1439 are separated by 256 nucleotides Shewana3_1439 and Shewana3_1440 are separated by 269 nucleotides Shewana3_1440 and Shewana3_1441 are separated by 34 nucleotides
Shewana3_1438: secF - preprotein translocase subunit SecF (RefSeq), at 1,667,661 to 1,668,608
secF
Shewana3_1439: Shewana3_1439 - hypothetical protein (RefSeq), at 1,668,865 to 1,669,182
_1439
Shewana3_1440: Shewana3_1440 - rhodanese domain-containing protein (RefSeq), at 1,669,452 to 1,669,811
_1440
Shewana3_1441: Shewana3_1441 - siroheme synthase (RefSeq), at 1,669,846 to 1,670,757
_1441
Position (kb) 1669
1670 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1668.828 kb on - strand at 1669.109 kb on + strand, within Shewana3_1439 at 1669.332 kb on - strand at 1669.463 kb on - strand at 1669.489 kb on - strand, within Shewana3_1440 at 1669.636 kb on + strand, within Shewana3_1440 at 1669.995 kb on + strand, within Shewana3_1441 at 1670.242 kb on - strand, within Shewana3_1441 at 1670.320 kb on + strand, within Shewana3_1441 at 1670.360 kb on - strand, within Shewana3_1441 at 1670.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,668,828 - -1.3 1,669,109 + Shewana3_1439 0.77 -0.9 1,669,332 - -1.2 1,669,463 - -2.9 1,669,489 - Shewana3_1440 0.10 +0.2 1,669,636 + Shewana3_1440 0.51 +0.1 1,669,995 + Shewana3_1441 0.16 -4.2 1,670,242 - Shewana3_1441 0.43 -4.4 1,670,320 + Shewana3_1441 0.52 -5.4 1,670,360 - Shewana3_1441 0.56 -5.0 1,670,801 - +0.5
Or see this region's nucleotide sequence