Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1417 and Shewana3_R0054 are separated by 143 nucleotides Shewana3_R0054 and Shewana3_R0055 are separated by 25 nucleotides Shewana3_R0055 and Shewana3_R0056 are separated by 25 nucleotides Shewana3_R0056 and Shewana3_R0057 are separated by 41 nucleotides Shewana3_R0057 and Shewana3_R0058 are separated by 32 nucleotides Shewana3_R0058 and Shewana3_R0059 are separated by 48 nucleotides Shewana3_R0059 and gltX are separated by 588 nucleotides
Shewana3_1417: Shewana3_1417 - hypothetical protein (RefSeq), at 1,642,249 to 1,642,479
_1417
Shewana3_R0054: Shewana3_R0054 - tRNA-Val (RefSeq), at 1,642,623 to 1,642,698
_R0054
Shewana3_R0055: Shewana3_R0055 - tRNA-Val (RefSeq), at 1,642,724 to 1,642,799
_R0055
Shewana3_R0056: Shewana3_R0056 - tRNA-Val (RefSeq), at 1,642,825 to 1,642,900
_R0056
Shewana3_R0057: Shewana3_R0057 - tRNA-Val (RefSeq), at 1,642,942 to 1,643,017
_R0057
Shewana3_R0058: Shewana3_R0058 - tRNA-Val (RefSeq), at 1,643,050 to 1,643,125
_R0058
Shewana3_R0059: Shewana3_R0059 - tRNA-Val (RefSeq), at 1,643,174 to 1,643,249
_R0059
Shewana3_1418: gltX - glutamyl-tRNA synthetase (RefSeq), at 1,643,838 to 1,645,247
gltX
Position (kb)
1642
1643
1644 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1641.964 kb on + strand at 1641.972 kb on - strand at 1642.233 kb on - strand at 1642.414 kb on + strand, within Shewana3_1417 at 1642.507 kb on - strand at 1643.424 kb on - strand at 1643.538 kb on - strand at 1643.697 kb on + strand at 1643.697 kb on + strand at 1643.697 kb on + strand at 1643.698 kb on - strand at 1643.705 kb on - strand at 1643.705 kb on - strand at 1643.715 kb on - strand at 1643.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,641,964 + -0.3 1,641,972 - -0.6 1,642,233 - -0.4 1,642,414 + Shewana3_1417 0.71 +1.2 1,642,507 - +1.2 1,643,424 - -1.1 1,643,538 - -0.5 1,643,697 + +0.2 1,643,697 + +0.7 1,643,697 + -1.9 1,643,698 - -0.2 1,643,705 - +0.4 1,643,705 - +0.4 1,643,715 - +0.3 1,643,758 + +3.0
Or see this region's nucleotide sequence