Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0044 and Shewana3_R0045 are separated by 43 nucleotides Shewana3_R0045 and Shewana3_R0046 are separated by 44 nucleotides Shewana3_R0046 and Shewana3_R0047 are separated by 36 nucleotides Shewana3_R0047 and Shewana3_R0048 are separated by 94 nucleotides Shewana3_R0048 and valS are separated by 406 nucleotides valS and Shewana3_1132 are separated by 15 nucleotides
Shewana3_R0044: Shewana3_R0044 - tRNA-Arg (RefSeq), at 1,333,015 to 1,333,091
_R0044
Shewana3_R0045: Shewana3_R0045 - tRNA-Arg (RefSeq), at 1,333,135 to 1,333,211
_R0045
Shewana3_R0046: Shewana3_R0046 - tRNA-Arg (RefSeq), at 1,333,256 to 1,333,332
_R0046
Shewana3_R0047: Shewana3_R0047 - tRNA-Ser (RefSeq), at 1,333,369 to 1,333,460
_R0047
Shewana3_R0048: Shewana3_R0048 - tRNA-Arg (RefSeq), at 1,333,555 to 1,333,631
_R0048
Shewana3_1131: valS - valyl-tRNA synthetase (RefSeq), at 1,334,038 to 1,336,914
valS
Shewana3_1132: Shewana3_1132 - DNA polymerase III chi subunit, HolC (RefSeq), at 1,336,930 to 1,337,394
_1132
Position (kb)
1334
1335
1336
1337 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1333.713 kb on + strand at 1333.853 kb on + strand at 1333.901 kb on + strand at 1333.957 kb on + strand at 1333.957 kb on + strand at 1334.036 kb on - strand at 1334.050 kb on - strand at 1337.372 kb on + strand at 1337.380 kb on - strand at 1337.409 kb on + strand at 1337.520 kb on - strand at 1337.528 kb on + strand at 1337.537 kb on - strand at 1337.564 kb on - strand at 1337.601 kb on + strand at 1337.630 kb on - strand at 1337.632 kb on + strand at 1337.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,333,713 + -0.8 1,333,853 + -0.8 1,333,901 + +0.5 1,333,957 + -1.4 1,333,957 + +1.2 1,334,036 - +4.1 1,334,050 - +0.7 1,337,372 + +1.9 1,337,380 - -1.5 1,337,409 + +2.1 1,337,520 - +0.7 1,337,528 + +0.0 1,337,537 - -0.9 1,337,564 - +0.5 1,337,601 + +0.3 1,337,630 - +0.3 1,337,632 + +0.6 1,337,855 + +0.1
Or see this region's nucleotide sequence