Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribH and nusB are separated by 14 nucleotides nusB and Shewana3_1101 are separated by 78 nucleotides Shewana3_1101 and Shewana3_1102 overlap by 1 nucleotides Shewana3_1102 and Shewana3_1103 are separated by 70 nucleotides
Shewana3_1099: ribH - 6,7-dimethyl-8-ribityllumazine synthase (RefSeq), at 1,293,461 to 1,294,003
ribH
Shewana3_1100: nusB - transcription antitermination protein NusB (RefSeq), at 1,294,018 to 1,294,422
nusB
Shewana3_1101: Shewana3_1101 - thiamine-monophosphate kinase (RefSeq), at 1,294,501 to 1,295,457
_1101
Shewana3_1102: Shewana3_1102 - phosphatidylglycerophosphatase (RefSeq), at 1,295,457 to 1,295,951
_1102
Shewana3_1103: Shewana3_1103 - hypothetical protein (RefSeq), at 1,296,022 to 1,296,897
_1103
Position (kb)
1294
1295
1296 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1294.491 kb on + strand at 1296.057 kb on - strand at 1296.117 kb on - strand, within Shewana3_1103 at 1296.159 kb on + strand, within Shewana3_1103 at 1296.303 kb on + strand, within Shewana3_1103 at 1296.371 kb on + strand, within Shewana3_1103 at 1296.436 kb on + strand, within Shewana3_1103 at 1296.441 kb on + strand, within Shewana3_1103 at 1296.441 kb on + strand, within Shewana3_1103 at 1296.449 kb on - strand, within Shewana3_1103
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,294,491 + +0.4 1,296,057 - -0.3 1,296,117 - Shewana3_1103 0.11 +1.1 1,296,159 + Shewana3_1103 0.16 +0.3 1,296,303 + Shewana3_1103 0.32 +0.7 1,296,371 + Shewana3_1103 0.40 +1.9 1,296,436 + Shewana3_1103 0.47 +0.6 1,296,441 + Shewana3_1103 0.48 -0.8 1,296,441 + Shewana3_1103 0.48 -1.1 1,296,449 - Shewana3_1103 0.49 -3.3
Or see this region's nucleotide sequence